Parafusin and calcium-induced signal transduction in exocytosis

Birgit H. Satir, H. Zhao

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The phosphoglycoprotein parafusin is a member of the phosphoglucomutase superfamily and has been shown, both via biochemical and localization studies, to be associated with the Ca2+dependent regulated exocytosis process in Paramecium tetraurelia. Stimulation of exocytosis in this cell leads to a Ca2+-dependent glucosylation of parafusin accompanied by its dissociation from the secretory vesicles and from cell membrane docking sites. These events are blocked in the presence of extracellular Mg2+ in wild-type cells and in either Ca2+ or Mg2+ in a temperature-sensitive mutant, nd9, stimulated at the nonpermissive temperature. Furthermore, laser scanning confocal localization studies with antibodies to parafusin whole protein versus antibody made to a specific peptide (insertion 2) show different localization patterns. While insertion-2 antibodies only label the organelles previously shown to have parafusin associated with them, i.e., cell membrane fusion (docking) sites and secretory vesicles, antibodies to whole protein outline in addition the alveolar sacs (subsurface cisterns) which are Ca2+ storage compartments in this cell. This may indicate tht other members of the phosphoglucomutase superfamily which interact specifically with this compartment are present in P. tetraurelia.

Original languageEnglish (US)
Pages (from-to)228-233
Number of pages6
JournalProtoplasma
Volume206
Issue number4
StatePublished - 1999

Fingerprint

exocytosis
Exocytosis
signal transduction
Signal Transduction
Paramecium tetraurelia
Phosphoglucomutase
Calcium
calcium
phosphoglucomutase
antibodies
Antibodies
secretory granules
Secretory Vesicles
cell membranes
Cell Membrane
Membrane Fusion
Temperature
Cell Fusion
cells
Organelles

Keywords

  • Exocytosis
  • Parafusin
  • Paramecium
  • Phosphoglucomutase superfamily

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Satir, B. H., & Zhao, H. (1999). Parafusin and calcium-induced signal transduction in exocytosis. Protoplasma, 206(4), 228-233.

Parafusin and calcium-induced signal transduction in exocytosis. / Satir, Birgit H.; Zhao, H.

In: Protoplasma, Vol. 206, No. 4, 1999, p. 228-233.

Research output: Contribution to journalArticle

Satir, BH & Zhao, H 1999, 'Parafusin and calcium-induced signal transduction in exocytosis', Protoplasma, vol. 206, no. 4, pp. 228-233.
Satir, Birgit H. ; Zhao, H. / Parafusin and calcium-induced signal transduction in exocytosis. In: Protoplasma. 1999 ; Vol. 206, No. 4. pp. 228-233.
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