PAH-DNA adducts in a Chinese population: Relationship to PAH exposure, smoking and polymorphisms of metabolic and DNA repair genes

Yu Hu, Gang Li, Xiaonan (Nan) Xue, Zongcan Zhou, Xiaomei Li, Juanling Fu, Beverly Cohen, Nirmal Roy, Donghui Li, Jianya Sun, Peihong Nan, Moon Shong Tang, Qingshan Qu

Research output: Contribution to journalArticle

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Abstract

The present study was conducted in a Chinese population to evaluate the usefulness and sensitivity of PAH-DNA adduct as a biomarker of PAH exposure, and to examine the potential effects of smoking and polymorphisms of responsive genes on DNA adduct formation induced by PAH exposure. The polymorphisms of genes examined include GSTM1, GSTT1, CYP1A1, microsomal epoxide hydrolase (mEH) and excision repair cross-complementary group 2 (ERCC2). A total of 194 subjects with a broad range of PAH exposures were recruited, including 116 occupationally exposed workers, 49 metropolitan residents and 29 suburban gardeners. A significant exposure-response relationship was observed between PAH exposure and DNA adducts in leukocytes across the entire group of subjects (p<0.0001). The levels of PAH-DNA adducts in the subgroup with lowest occupational exposure to PAHs (<0.1μg BaP m-3) was significantly higher than that in metropolitan residents and suburban gardeners. However, no significant difference was detected between residents and gardeners, with mean BaP concentrations of 0.028 and 0.011 μg m-3, respectively. The polymorphisms of genes examined failed to show significant effects on PAH-induced adduct formation except ERCC2 Lys751Gln genotypes. A significantly higher level of PAH-DNA adduct was found in subjects with wild-type ERCC2 than those who have either heterozygous or homozygous variant alleles (p<0.01). Smoking, age and gender did not substantially contribute to PAH-induced DNA adduct formation in this study. The study suggests that PAH-DNA adducts may serve as a reliable biomarker of PAH exposure in occupational settings but may not be sensitive enough to be used in populations with environmental exposures to PAHs.

Original languageEnglish (US)
Pages (from-to)27-40
Number of pages14
JournalBiomarkers
Volume13
Issue number1
DOIs
StatePublished - Feb 2008

Fingerprint

Polycyclic aromatic hydrocarbons
Polymorphism
DNA Repair
Repair
Genes
Smoking
DNA
Population
Occupational Exposure
DNA Adducts
Biomarkers
Epoxide Hydrolases
Cytochrome P-450 CYP1A1
Environmental Exposure
polycyclic aromatic hydrocarbons-DNA adduct
Leukocytes
Alleles
Genotype

Keywords

  • Biomarkers
  • Environmental exposure
  • Genetic susceptibility
  • Polycyclic aromatic hydrocarbons

ASJC Scopus subject areas

  • Biotechnology
  • Toxicology

Cite this

PAH-DNA adducts in a Chinese population : Relationship to PAH exposure, smoking and polymorphisms of metabolic and DNA repair genes. / Hu, Yu; Li, Gang; Xue, Xiaonan (Nan); Zhou, Zongcan; Li, Xiaomei; Fu, Juanling; Cohen, Beverly; Roy, Nirmal; Li, Donghui; Sun, Jianya; Nan, Peihong; Tang, Moon Shong; Qu, Qingshan.

In: Biomarkers, Vol. 13, No. 1, 02.2008, p. 27-40.

Research output: Contribution to journalArticle

Hu, Y, Li, G, Xue, XN, Zhou, Z, Li, X, Fu, J, Cohen, B, Roy, N, Li, D, Sun, J, Nan, P, Tang, MS & Qu, Q 2008, 'PAH-DNA adducts in a Chinese population: Relationship to PAH exposure, smoking and polymorphisms of metabolic and DNA repair genes', Biomarkers, vol. 13, no. 1, pp. 27-40. https://doi.org/10.1080/13547500701671895
Hu, Yu ; Li, Gang ; Xue, Xiaonan (Nan) ; Zhou, Zongcan ; Li, Xiaomei ; Fu, Juanling ; Cohen, Beverly ; Roy, Nirmal ; Li, Donghui ; Sun, Jianya ; Nan, Peihong ; Tang, Moon Shong ; Qu, Qingshan. / PAH-DNA adducts in a Chinese population : Relationship to PAH exposure, smoking and polymorphisms of metabolic and DNA repair genes. In: Biomarkers. 2008 ; Vol. 13, No. 1. pp. 27-40.
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