Oxygenated mycolic acids are necessary for virulence of Mycobacterium tuberculosis in mice

Eugenie Dubnau, John Chan, Catherine Raynaud, Vellore P. Mohan, Marie Antoinette Lanéelle, Keming Yu, Annaik Quémard, Issar Smith, Mamadou Daffé

Research output: Contribution to journalArticle

216 Citations (Scopus)

Abstract

Members of the Mycobacterium tuberculosis group synthesize a family of long-chain fatty acids, mycolic acids, which are located in the cell envelope. These include the non-oxygenated α-mycolic acid and the oxygenated keto- and methoxymycolic acids. The function in bacterial virulence, if any, of these various types of mycolic acids is unknown. We have constructed a mutant strain of M. tuberculosis with an inactivated hma (cmaA, mma4) gene; this mutant strain no longer synthesizes oxygenated mycolic acids, has profound alterations in its envelope permeability and is attenuated in mice.

Original languageEnglish (US)
Pages (from-to)630-637
Number of pages8
JournalMolecular Microbiology
Volume36
Issue number3
DOIs
StatePublished - 2000

Fingerprint

Mycolic Acids
Mycobacterium tuberculosis
Virulence
Keto Acids
Permeability
Fatty Acids
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Oxygenated mycolic acids are necessary for virulence of Mycobacterium tuberculosis in mice. / Dubnau, Eugenie; Chan, John; Raynaud, Catherine; Mohan, Vellore P.; Lanéelle, Marie Antoinette; Yu, Keming; Quémard, Annaik; Smith, Issar; Daffé, Mamadou.

In: Molecular Microbiology, Vol. 36, No. 3, 2000, p. 630-637.

Research output: Contribution to journalArticle

Dubnau, E, Chan, J, Raynaud, C, Mohan, VP, Lanéelle, MA, Yu, K, Quémard, A, Smith, I & Daffé, M 2000, 'Oxygenated mycolic acids are necessary for virulence of Mycobacterium tuberculosis in mice', Molecular Microbiology, vol. 36, no. 3, pp. 630-637. https://doi.org/10.1046/j.1365-2958.2000.01882.x
Dubnau, Eugenie ; Chan, John ; Raynaud, Catherine ; Mohan, Vellore P. ; Lanéelle, Marie Antoinette ; Yu, Keming ; Quémard, Annaik ; Smith, Issar ; Daffé, Mamadou. / Oxygenated mycolic acids are necessary for virulence of Mycobacterium tuberculosis in mice. In: Molecular Microbiology. 2000 ; Vol. 36, No. 3. pp. 630-637.
@article{0eda12c8eec741c98c11e5ff762faf5d,
title = "Oxygenated mycolic acids are necessary for virulence of Mycobacterium tuberculosis in mice",
abstract = "Members of the Mycobacterium tuberculosis group synthesize a family of long-chain fatty acids, mycolic acids, which are located in the cell envelope. These include the non-oxygenated α-mycolic acid and the oxygenated keto- and methoxymycolic acids. The function in bacterial virulence, if any, of these various types of mycolic acids is unknown. We have constructed a mutant strain of M. tuberculosis with an inactivated hma (cmaA, mma4) gene; this mutant strain no longer synthesizes oxygenated mycolic acids, has profound alterations in its envelope permeability and is attenuated in mice.",
author = "Eugenie Dubnau and John Chan and Catherine Raynaud and Mohan, {Vellore P.} and Lan{\'e}elle, {Marie Antoinette} and Keming Yu and Annaik Qu{\'e}mard and Issar Smith and Mamadou Daff{\'e}",
year = "2000",
doi = "10.1046/j.1365-2958.2000.01882.x",
language = "English (US)",
volume = "36",
pages = "630--637",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Oxygenated mycolic acids are necessary for virulence of Mycobacterium tuberculosis in mice

AU - Dubnau, Eugenie

AU - Chan, John

AU - Raynaud, Catherine

AU - Mohan, Vellore P.

AU - Lanéelle, Marie Antoinette

AU - Yu, Keming

AU - Quémard, Annaik

AU - Smith, Issar

AU - Daffé, Mamadou

PY - 2000

Y1 - 2000

N2 - Members of the Mycobacterium tuberculosis group synthesize a family of long-chain fatty acids, mycolic acids, which are located in the cell envelope. These include the non-oxygenated α-mycolic acid and the oxygenated keto- and methoxymycolic acids. The function in bacterial virulence, if any, of these various types of mycolic acids is unknown. We have constructed a mutant strain of M. tuberculosis with an inactivated hma (cmaA, mma4) gene; this mutant strain no longer synthesizes oxygenated mycolic acids, has profound alterations in its envelope permeability and is attenuated in mice.

AB - Members of the Mycobacterium tuberculosis group synthesize a family of long-chain fatty acids, mycolic acids, which are located in the cell envelope. These include the non-oxygenated α-mycolic acid and the oxygenated keto- and methoxymycolic acids. The function in bacterial virulence, if any, of these various types of mycolic acids is unknown. We have constructed a mutant strain of M. tuberculosis with an inactivated hma (cmaA, mma4) gene; this mutant strain no longer synthesizes oxygenated mycolic acids, has profound alterations in its envelope permeability and is attenuated in mice.

UR - http://www.scopus.com/inward/record.url?scp=0034073375&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034073375&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2958.2000.01882.x

DO - 10.1046/j.1365-2958.2000.01882.x

M3 - Article

C2 - 10844652

AN - SCOPUS:0034073375

VL - 36

SP - 630

EP - 637

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 3

ER -