Oxalobacter formigenes-Derived Bioactive Factors Stimulate Oxalate Transport by Intestinal Epithelial Cells

Donna Arvans, Yong Chul Jung, Dionysios Antonopoulos, Jason Koval, Ignacio Granja, Mohamed Bashir, Eltayeb Karrar, Jayanta Roy-Chowdhury, Mark Musch, John Asplin, Eugene Chang, Hatim Hassan

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Hyperoxaluria is a major risk factor for kidney stones and has no specific therapy, although Oxalobacter formigenes colonization is associated with reduced stone risk. O. formigenes interacts with colonic epithelium and induces colonic oxalate secretion, thereby reducing urinary oxalate excretion, via an unknown secretagogue. The difficulties in sustaining O. formigenes colonization underscore the need to identify the derived factors inducing colonic oxalate secretion. We therefore evaluated the effects of O. formigenes culture conditioned medium (CM) on apical 14C-oxalate uptake by human intestinal Caco-2-BBE cells. Compared with control medium, O. formigenes CM significantly stimulated oxalate uptake (>2.4-fold), whereas CM from Lactobacillus acidophilus did not. Treating the O. formigenes CM with heat or pepsin completely abolished this bioactivity, and selective ultrafiltration of the CM revealed that the O. formigenes-derived factors have molecular masses of 10-30 kDa. Treatment with the protein kinase A inhibitor H89 or the anion exchange inhibitor 4,4'-diisothiocyano-2,2'-stilbenedisulfonic acid completely blocked the CM-induced oxalate transport. Knockdown of the oxalate transporter SLC26A6 also significantly restricted the induction of oxalate transport by CM. In a mouse model of primary hyperoxaluria type 1, rectal administration of O. formigenes CM significantly reduced (>32.5%) urinary oxalate excretion and stimulated (>42%) distal colonic oxalate secretion. We conclude that O. formigenes-derived bioactive factors stimulate oxalate transport in intestinal cells through mechanisms including PKA activation. The reduction in urinary oxalate excretion in hyperoxaluric mice treated with O. formigenes CM reflects the in vivo retention of biologic activity and the therapeutic potential of these factors.

Original languageEnglish (US)
Pages (from-to)876-887
Number of pages12
JournalJournal of the American Society of Nephrology : JASN
Volume28
Issue number3
DOIs
StatePublished - Mar 1 2017

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Oxalobacter formigenes
Oxalates
Conditioned Culture Medium
Epithelial Cells
Rectal Administration
Hyperoxaluria
Lactobacillus acidophilus
Kidney Calculi
Caco-2 Cells
Pepsin A
Ultrafiltration

Keywords

  • intestinal oxalate transport
  • Oxalobacter formigenes
  • PKA
  • secreted bioactive factors
  • SLC26A6

ASJC Scopus subject areas

  • Nephrology

Cite this

Oxalobacter formigenes-Derived Bioactive Factors Stimulate Oxalate Transport by Intestinal Epithelial Cells. / Arvans, Donna; Jung, Yong Chul; Antonopoulos, Dionysios; Koval, Jason; Granja, Ignacio; Bashir, Mohamed; Karrar, Eltayeb; Roy-Chowdhury, Jayanta; Musch, Mark; Asplin, John; Chang, Eugene; Hassan, Hatim.

In: Journal of the American Society of Nephrology : JASN, Vol. 28, No. 3, 01.03.2017, p. 876-887.

Research output: Contribution to journalArticle

Arvans, D, Jung, YC, Antonopoulos, D, Koval, J, Granja, I, Bashir, M, Karrar, E, Roy-Chowdhury, J, Musch, M, Asplin, J, Chang, E & Hassan, H 2017, 'Oxalobacter formigenes-Derived Bioactive Factors Stimulate Oxalate Transport by Intestinal Epithelial Cells', Journal of the American Society of Nephrology : JASN, vol. 28, no. 3, pp. 876-887. https://doi.org/10.1681/ASN.2016020132
Arvans, Donna ; Jung, Yong Chul ; Antonopoulos, Dionysios ; Koval, Jason ; Granja, Ignacio ; Bashir, Mohamed ; Karrar, Eltayeb ; Roy-Chowdhury, Jayanta ; Musch, Mark ; Asplin, John ; Chang, Eugene ; Hassan, Hatim. / Oxalobacter formigenes-Derived Bioactive Factors Stimulate Oxalate Transport by Intestinal Epithelial Cells. In: Journal of the American Society of Nephrology : JASN. 2017 ; Vol. 28, No. 3. pp. 876-887.
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