Overexpression of Trpp5 contributes to cell proliferation and apoptosis probably through involving calcium homeostasis

Yan Xiao, Xiaoyan Lv, Ge Cao, Guohui Bian, Jingjing Duan, Jianzhong Ai, Huan Sun, Qingwei Li, Qiutan Yang, Tielin Chen, Danhua Zhao, Ruizhi Tan, Yuhang Liu, Yidong Wang, Zheng Zhang, Yang Yang, Yuquan Wei, Qin Zhou

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Trpp5 is one member of the polycystic kidney disease (PKD) family, which belongs to transient receptor potential (TRP) superfamily. Our previous study has shown that Trpp5 is developmentally expressed in mouse testis and overexpression of Trpp5 increases intracellular free calcium concentration in MDCK cells. However, the roles of this protein in cellular processes are largely unknown. Here, we demonstrated that Trpp5 resided in both cytoplasm and cell membrane of HEK293 cells. We found that overexpression of Trpp5 slightly increased the calcium current amplitude of HEK293 cells and shifted the reversal potential to a more negative value. Meanwhile, overexpression of Trpp5 suppressed proliferation of Hela cells via inhibiting DNA replication and induced apoptosis of Hela cells with morphological changes and accumulation of fragmented DNA. Collectively, these findings suggest that Trpp5 might involve calcium homeostasis contributing to cell proliferation and apoptosis.

Original languageEnglish (US)
Pages (from-to)155-161
Number of pages7
JournalMolecular and Cellular Biochemistry
Volume339
Issue number1-2
DOIs
StatePublished - Jun 2010
Externally publishedYes

Fingerprint

Cell proliferation
Homeostasis
HEK293 Cells
Cell Proliferation
Apoptosis
Calcium
HeLa Cells
Polycystic Kidney Diseases
Madin Darby Canine Kidney Cells
DNA
Cell membranes
DNA Replication
Testis
Cytoplasm
Cell Membrane
Proteins

Keywords

  • Apoptosis
  • Calcium
  • Cell proliferation
  • PKD
  • TRP channel
  • Trpp5

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Overexpression of Trpp5 contributes to cell proliferation and apoptosis probably through involving calcium homeostasis. / Xiao, Yan; Lv, Xiaoyan; Cao, Ge; Bian, Guohui; Duan, Jingjing; Ai, Jianzhong; Sun, Huan; Li, Qingwei; Yang, Qiutan; Chen, Tielin; Zhao, Danhua; Tan, Ruizhi; Liu, Yuhang; Wang, Yidong; Zhang, Zheng; Yang, Yang; Wei, Yuquan; Zhou, Qin.

In: Molecular and Cellular Biochemistry, Vol. 339, No. 1-2, 06.2010, p. 155-161.

Research output: Contribution to journalArticle

Xiao, Y, Lv, X, Cao, G, Bian, G, Duan, J, Ai, J, Sun, H, Li, Q, Yang, Q, Chen, T, Zhao, D, Tan, R, Liu, Y, Wang, Y, Zhang, Z, Yang, Y, Wei, Y & Zhou, Q 2010, 'Overexpression of Trpp5 contributes to cell proliferation and apoptosis probably through involving calcium homeostasis', Molecular and Cellular Biochemistry, vol. 339, no. 1-2, pp. 155-161. https://doi.org/10.1007/s11010-009-0379-8
Xiao, Yan ; Lv, Xiaoyan ; Cao, Ge ; Bian, Guohui ; Duan, Jingjing ; Ai, Jianzhong ; Sun, Huan ; Li, Qingwei ; Yang, Qiutan ; Chen, Tielin ; Zhao, Danhua ; Tan, Ruizhi ; Liu, Yuhang ; Wang, Yidong ; Zhang, Zheng ; Yang, Yang ; Wei, Yuquan ; Zhou, Qin. / Overexpression of Trpp5 contributes to cell proliferation and apoptosis probably through involving calcium homeostasis. In: Molecular and Cellular Biochemistry. 2010 ; Vol. 339, No. 1-2. pp. 155-161.
@article{7818871dc90a4433ae068ddc2b035529,
title = "Overexpression of Trpp5 contributes to cell proliferation and apoptosis probably through involving calcium homeostasis",
abstract = "Trpp5 is one member of the polycystic kidney disease (PKD) family, which belongs to transient receptor potential (TRP) superfamily. Our previous study has shown that Trpp5 is developmentally expressed in mouse testis and overexpression of Trpp5 increases intracellular free calcium concentration in MDCK cells. However, the roles of this protein in cellular processes are largely unknown. Here, we demonstrated that Trpp5 resided in both cytoplasm and cell membrane of HEK293 cells. We found that overexpression of Trpp5 slightly increased the calcium current amplitude of HEK293 cells and shifted the reversal potential to a more negative value. Meanwhile, overexpression of Trpp5 suppressed proliferation of Hela cells via inhibiting DNA replication and induced apoptosis of Hela cells with morphological changes and accumulation of fragmented DNA. Collectively, these findings suggest that Trpp5 might involve calcium homeostasis contributing to cell proliferation and apoptosis.",
keywords = "Apoptosis, Calcium, Cell proliferation, PKD, TRP channel, Trpp5",
author = "Yan Xiao and Xiaoyan Lv and Ge Cao and Guohui Bian and Jingjing Duan and Jianzhong Ai and Huan Sun and Qingwei Li and Qiutan Yang and Tielin Chen and Danhua Zhao and Ruizhi Tan and Yuhang Liu and Yidong Wang and Zheng Zhang and Yang Yang and Yuquan Wei and Qin Zhou",
year = "2010",
month = "6",
doi = "10.1007/s11010-009-0379-8",
language = "English (US)",
volume = "339",
pages = "155--161",
journal = "Molecular and Cellular Biochemistry",
issn = "0300-8177",
publisher = "Springer Netherlands",
number = "1-2",

}

TY - JOUR

T1 - Overexpression of Trpp5 contributes to cell proliferation and apoptosis probably through involving calcium homeostasis

AU - Xiao, Yan

AU - Lv, Xiaoyan

AU - Cao, Ge

AU - Bian, Guohui

AU - Duan, Jingjing

AU - Ai, Jianzhong

AU - Sun, Huan

AU - Li, Qingwei

AU - Yang, Qiutan

AU - Chen, Tielin

AU - Zhao, Danhua

AU - Tan, Ruizhi

AU - Liu, Yuhang

AU - Wang, Yidong

AU - Zhang, Zheng

AU - Yang, Yang

AU - Wei, Yuquan

AU - Zhou, Qin

PY - 2010/6

Y1 - 2010/6

N2 - Trpp5 is one member of the polycystic kidney disease (PKD) family, which belongs to transient receptor potential (TRP) superfamily. Our previous study has shown that Trpp5 is developmentally expressed in mouse testis and overexpression of Trpp5 increases intracellular free calcium concentration in MDCK cells. However, the roles of this protein in cellular processes are largely unknown. Here, we demonstrated that Trpp5 resided in both cytoplasm and cell membrane of HEK293 cells. We found that overexpression of Trpp5 slightly increased the calcium current amplitude of HEK293 cells and shifted the reversal potential to a more negative value. Meanwhile, overexpression of Trpp5 suppressed proliferation of Hela cells via inhibiting DNA replication and induced apoptosis of Hela cells with morphological changes and accumulation of fragmented DNA. Collectively, these findings suggest that Trpp5 might involve calcium homeostasis contributing to cell proliferation and apoptosis.

AB - Trpp5 is one member of the polycystic kidney disease (PKD) family, which belongs to transient receptor potential (TRP) superfamily. Our previous study has shown that Trpp5 is developmentally expressed in mouse testis and overexpression of Trpp5 increases intracellular free calcium concentration in MDCK cells. However, the roles of this protein in cellular processes are largely unknown. Here, we demonstrated that Trpp5 resided in both cytoplasm and cell membrane of HEK293 cells. We found that overexpression of Trpp5 slightly increased the calcium current amplitude of HEK293 cells and shifted the reversal potential to a more negative value. Meanwhile, overexpression of Trpp5 suppressed proliferation of Hela cells via inhibiting DNA replication and induced apoptosis of Hela cells with morphological changes and accumulation of fragmented DNA. Collectively, these findings suggest that Trpp5 might involve calcium homeostasis contributing to cell proliferation and apoptosis.

KW - Apoptosis

KW - Calcium

KW - Cell proliferation

KW - PKD

KW - TRP channel

KW - Trpp5

UR - http://www.scopus.com/inward/record.url?scp=77952290935&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952290935&partnerID=8YFLogxK

U2 - 10.1007/s11010-009-0379-8

DO - 10.1007/s11010-009-0379-8

M3 - Article

VL - 339

SP - 155

EP - 161

JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

SN - 0300-8177

IS - 1-2

ER -