Overexpression of Trpp5 contributes to cell proliferation and apoptosis probably through involving calcium homeostasis

Yan Xiao; Xiaoyan Lv; Ge Cao; Guohui Bian; Jingjing Duan; Jianzhong Ai; Huan Sun; Qingwei Li; Qiutan Yang; Tielin Chen; Danhua Zhao; Ruizhi Tan; Yuhang Liu; Yidong Wang; Zheng Zhang; Yang Yang; Yuquan Wei; Qin Zhou

doi:10.1007/s11010-009-0379-8

Overexpression of Trpp5 contributes to cell proliferation and apoptosis probably through involving calcium homeostasis

Yan Xiao, Xiaoyan Lv, Ge Cao, Guohui Bian, Jingjing Duan, Jianzhong Ai, Huan Sun, Qingwei Li, Qiutan Yang, Tielin Chen, Danhua Zhao, Ruizhi Tan, Yuhang Liu, Yidong Wang, Zheng Zhang, Yang Yang, Yuquan Wei, Qin Zhou

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Trpp5 is one member of the polycystic kidney disease (PKD) family, which belongs to transient receptor potential (TRP) superfamily. Our previous study has shown that Trpp5 is developmentally expressed in mouse testis and overexpression of Trpp5 increases intracellular free calcium concentration in MDCK cells. However, the roles of this protein in cellular processes are largely unknown. Here, we demonstrated that Trpp5 resided in both cytoplasm and cell membrane of HEK293 cells. We found that overexpression of Trpp5 slightly increased the calcium current amplitude of HEK293 cells and shifted the reversal potential to a more negative value. Meanwhile, overexpression of Trpp5 suppressed proliferation of Hela cells via inhibiting DNA replication and induced apoptosis of Hela cells with morphological changes and accumulation of fragmented DNA. Collectively, these findings suggest that Trpp5 might involve calcium homeostasis contributing to cell proliferation and apoptosis.

Original language	English (US)
Pages (from-to)	155-161
Number of pages	7
Journal	Molecular and Cellular Biochemistry
Volume	339
Issue number	1-2
DOIs	https://doi.org/10.1007/s11010-009-0379-8
State	Published - Jun 2010
Externally published	Yes

Keywords

Apoptosis
Calcium
Cell proliferation
PKD
TRP channel
Trpp5

ASJC Scopus subject areas

Molecular Biology
Clinical Biochemistry
Cell Biology

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10.1007/s11010-009-0379-8

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Xiao, Y., Lv, X., Cao, G., Bian, G., Duan, J., Ai, J., Sun, H., Li, Q., Yang, Q., Chen, T., Zhao, D., Tan, R., Liu, Y., Wang, Y., Zhang, Z., Yang, Y., Wei, Y., & Zhou, Q. (2010). Overexpression of Trpp5 contributes to cell proliferation and apoptosis probably through involving calcium homeostasis. Molecular and Cellular Biochemistry, 339(1-2), 155-161. https://doi.org/10.1007/s11010-009-0379-8

Xiao, Y, Lv, X, Cao, G, Bian, G, Duan, J, Ai, J, Sun, H, Li, Q, Yang, Q, Chen, T, Zhao, D, Tan, R, Liu, Y, Wang, Y, Zhang, Z, Yang, Y, Wei, Y & Zhou, Q 2010, 'Overexpression of Trpp5 contributes to cell proliferation and apoptosis probably through involving calcium homeostasis', Molecular and Cellular Biochemistry, vol. 339, no. 1-2, pp. 155-161. https://doi.org/10.1007/s11010-009-0379-8

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title = "Overexpression of Trpp5 contributes to cell proliferation and apoptosis probably through involving calcium homeostasis",

abstract = "Trpp5 is one member of the polycystic kidney disease (PKD) family, which belongs to transient receptor potential (TRP) superfamily. Our previous study has shown that Trpp5 is developmentally expressed in mouse testis and overexpression of Trpp5 increases intracellular free calcium concentration in MDCK cells. However, the roles of this protein in cellular processes are largely unknown. Here, we demonstrated that Trpp5 resided in both cytoplasm and cell membrane of HEK293 cells. We found that overexpression of Trpp5 slightly increased the calcium current amplitude of HEK293 cells and shifted the reversal potential to a more negative value. Meanwhile, overexpression of Trpp5 suppressed proliferation of Hela cells via inhibiting DNA replication and induced apoptosis of Hela cells with morphological changes and accumulation of fragmented DNA. Collectively, these findings suggest that Trpp5 might involve calcium homeostasis contributing to cell proliferation and apoptosis.",

keywords = "Apoptosis, Calcium, Cell proliferation, PKD, TRP channel, Trpp5",

author = "Yan Xiao and Xiaoyan Lv and Ge Cao and Guohui Bian and Jingjing Duan and Jianzhong Ai and Huan Sun and Qingwei Li and Qiutan Yang and Tielin Chen and Danhua Zhao and Ruizhi Tan and Yuhang Liu and Yidong Wang and Zheng Zhang and Yang Yang and Yuquan Wei and Qin Zhou",

note = "Funding Information: Acknowledgments We would like to thank Prof. Guangju Ji. This work was supported by the grant from National Key Basic Research Program of China (2005CB522506) and the Initial Foundation of M.O.E. for Returned Overseas Students (200711081818) to Prof. Qin Zhou M.D.& Ph.D. This work was also supported by the National Natural Science Foundation of China (30801134) to Ge Cao and by a grant from National Key Basic Research Program of China to academician Yuquan Wei (2004CB518800).",

year = "2010",

month = jun,

doi = "10.1007/s11010-009-0379-8",

language = "English (US)",

volume = "339",

pages = "155--161",

journal = "Molecular and Cellular Biochemistry",

issn = "0300-8177",

publisher = "Springer Netherlands",

number = "1-2",

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TY - JOUR

T1 - Overexpression of Trpp5 contributes to cell proliferation and apoptosis probably through involving calcium homeostasis

AU - Xiao, Yan

AU - Lv, Xiaoyan

AU - Cao, Ge

AU - Bian, Guohui

AU - Duan, Jingjing

AU - Ai, Jianzhong

AU - Sun, Huan

AU - Li, Qingwei

AU - Yang, Qiutan

AU - Chen, Tielin

AU - Zhao, Danhua

AU - Tan, Ruizhi

AU - Liu, Yuhang

AU - Wang, Yidong

AU - Zhang, Zheng

AU - Yang, Yang

AU - Wei, Yuquan

AU - Zhou, Qin

N1 - Funding Information: Acknowledgments We would like to thank Prof. Guangju Ji. This work was supported by the grant from National Key Basic Research Program of China (2005CB522506) and the Initial Foundation of M.O.E. for Returned Overseas Students (200711081818) to Prof. Qin Zhou M.D.& Ph.D. This work was also supported by the National Natural Science Foundation of China (30801134) to Ge Cao and by a grant from National Key Basic Research Program of China to academician Yuquan Wei (2004CB518800).

PY - 2010/6

Y1 - 2010/6

N2 - Trpp5 is one member of the polycystic kidney disease (PKD) family, which belongs to transient receptor potential (TRP) superfamily. Our previous study has shown that Trpp5 is developmentally expressed in mouse testis and overexpression of Trpp5 increases intracellular free calcium concentration in MDCK cells. However, the roles of this protein in cellular processes are largely unknown. Here, we demonstrated that Trpp5 resided in both cytoplasm and cell membrane of HEK293 cells. We found that overexpression of Trpp5 slightly increased the calcium current amplitude of HEK293 cells and shifted the reversal potential to a more negative value. Meanwhile, overexpression of Trpp5 suppressed proliferation of Hela cells via inhibiting DNA replication and induced apoptosis of Hela cells with morphological changes and accumulation of fragmented DNA. Collectively, these findings suggest that Trpp5 might involve calcium homeostasis contributing to cell proliferation and apoptosis.

AB - Trpp5 is one member of the polycystic kidney disease (PKD) family, which belongs to transient receptor potential (TRP) superfamily. Our previous study has shown that Trpp5 is developmentally expressed in mouse testis and overexpression of Trpp5 increases intracellular free calcium concentration in MDCK cells. However, the roles of this protein in cellular processes are largely unknown. Here, we demonstrated that Trpp5 resided in both cytoplasm and cell membrane of HEK293 cells. We found that overexpression of Trpp5 slightly increased the calcium current amplitude of HEK293 cells and shifted the reversal potential to a more negative value. Meanwhile, overexpression of Trpp5 suppressed proliferation of Hela cells via inhibiting DNA replication and induced apoptosis of Hela cells with morphological changes and accumulation of fragmented DNA. Collectively, these findings suggest that Trpp5 might involve calcium homeostasis contributing to cell proliferation and apoptosis.

KW - Apoptosis

KW - Calcium

KW - Cell proliferation

KW - PKD

KW - TRP channel

KW - Trpp5

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SN - 0300-8177

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EP - 161

JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

IS - 1-2

ER -

Overexpression of Trpp5 contributes to cell proliferation and apoptosis probably through involving calcium homeostasis

Abstract

Keywords

ASJC Scopus subject areas

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