Ovarian kisspeptin expression is related to age and to monocyte chemoattractant protein-1

Zaher Merhi, Kimberley Thornton, Elizabeth Bonney, Marilyn J. Cipolla, Maureen J. Charron, Erkan Buyuk

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose: The objective of this study was to test the hypothesis that ovarian kisspeptin (kiss1) and its receptor (kiss1r) expression are affected by age, obesity, and the age- and obesity-related chemokine monocyte chemoattractant protein-1 (MCP-1). Methods: Ovaries from reproductive-aged and older C57BL/6J mice fed normal chow (NC) or high-fat (HF) diet, ovaries from age-matched young MCP-1 knockout and young control mice on NC, and finally, cumulus and mural granulosa cells (GCs) from women who underwent in vitro fertilization (IVF) were collected. Kiss1, kiss1r, anti-Mullerian hormone (AMH), and AMH receptor (AMHR-II) messenger RNA (mRNA) expression levels were quantified using real-time polymerase chain reaction (RT-PCR). Results: In mouse ovaries, kiss1 and kiss1r mRNA levels were significantly higher in old compared to reproductive-aged mice, and diet-induced obesity did not alter kiss1 or kiss1r mRNA levels. Compared to young control mice, young MCP-1 knockout mice had significantly lower ovarian kiss1 mRNA but significantly higher AMH and AMHR-II mRNA levels. In human cumulus GCs, kiss1r mRNA levels were positively correlated with age but not with BMI. There was no expression of kiss1 mRNA in either cumulus or mural GCs. Conclusion: These data suggest a possible age-related physiologic role for the kisspeptinergic system in ovarian physiology. Additionally, the inflammatory MCP-1 may be associated with kiss1 and AMH genes, which are important in ovulation and folliculogenesis, respectively.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalJournal of Assisted Reproduction and Genetics
DOIs
StateAccepted/In press - Feb 15 2016

Fingerprint

Kisspeptins
Chemokine CCL2
Messenger RNA
Anti-Mullerian Hormone
Cumulus Cells
Ovary
Obesity
Granulosa Cells
High Fat Diet
Fertilization in Vitro
Ovulation
Inbred C57BL Mouse
Chemokines
Knockout Mice
Real-Time Polymerase Chain Reaction
Diet

Keywords

  • Aging
  • Kisspeptin
  • MCP-1
  • Obesity
  • Ovary

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine
  • Developmental Biology
  • Genetics
  • Genetics(clinical)

Cite this

Ovarian kisspeptin expression is related to age and to monocyte chemoattractant protein-1. / Merhi, Zaher; Thornton, Kimberley; Bonney, Elizabeth; Cipolla, Marilyn J.; Charron, Maureen J.; Buyuk, Erkan.

In: Journal of Assisted Reproduction and Genetics, 15.02.2016, p. 1-9.

Research output: Contribution to journalArticle

Merhi, Zaher ; Thornton, Kimberley ; Bonney, Elizabeth ; Cipolla, Marilyn J. ; Charron, Maureen J. ; Buyuk, Erkan. / Ovarian kisspeptin expression is related to age and to monocyte chemoattractant protein-1. In: Journal of Assisted Reproduction and Genetics. 2016 ; pp. 1-9.
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