O2 and CO reactions with heme proteins: Quantum yields and geminate recombination on picosecond time scales

Mark R. Chance, Scott H. Courtney, Joel M. Friedman, Mark R. Ondrias, Joel M. Friedman

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Picosecond time-resolved absorption spectroscopy and low-temperature studies have been undertaken in order to understand the nature of the intrinsic quantum yields and geminate recombination of carbon monoxide and oxygen to hemoglobin and myoglobin. We find that the photoproduct yields at 40 ps and long times (minutes) after photolysis at 8 K are similar; however, the yield of oxygen photoproducts is 0.4 ± 0.1 while the yield of carbon monoxide photoproducts is 1.0 ± 0.1 for both myoglobin and hemoglobin. Measurements in the Soret, near-infrared, and far-IR are used to quantitate the photoproduct yields. These results call into question previous cryogenic kinetic studies of O2 recombination. Significant subnanosecond geminate recombination is observed in oxyhemoglobin down to 150 K, while below 100 K this geminate recombination disappears. The lower photoproduct yields for oxyheme protein complexes can be attributed to both subnanosecond and subpicosecond recombination events which are ligand and protein dynamics dependent.

Original languageEnglish (US)
Pages (from-to)5537-5545
Number of pages9
JournalBiochemistry
Volume29
Issue number23
StatePublished - 1990
Externally publishedYes

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Hemeproteins
Myoglobin
Quantum yield
Carbon Monoxide
Genetic Recombination
Hemoglobins
Oxygen
Oxyhemoglobins
Photolysis
Absorption spectroscopy
Cryogenics
Proteins
Ligands
Infrared radiation
Kinetics
Spectrum Analysis
Temperature

ASJC Scopus subject areas

  • Biochemistry

Cite this

Chance, M. R., Courtney, S. H., Friedman, J. M., Ondrias, M. R., & Friedman, J. M. (1990). O2 and CO reactions with heme proteins: Quantum yields and geminate recombination on picosecond time scales. Biochemistry, 29(23), 5537-5545.

O2 and CO reactions with heme proteins : Quantum yields and geminate recombination on picosecond time scales. / Chance, Mark R.; Courtney, Scott H.; Friedman, Joel M.; Ondrias, Mark R.; Friedman, Joel M.

In: Biochemistry, Vol. 29, No. 23, 1990, p. 5537-5545.

Research output: Contribution to journalArticle

Chance, MR, Courtney, SH, Friedman, JM, Ondrias, MR & Friedman, JM 1990, 'O2 and CO reactions with heme proteins: Quantum yields and geminate recombination on picosecond time scales', Biochemistry, vol. 29, no. 23, pp. 5537-5545.
Chance, Mark R. ; Courtney, Scott H. ; Friedman, Joel M. ; Ondrias, Mark R. ; Friedman, Joel M. / O2 and CO reactions with heme proteins : Quantum yields and geminate recombination on picosecond time scales. In: Biochemistry. 1990 ; Vol. 29, No. 23. pp. 5537-5545.
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