TY - JOUR
T1 - Osterix marks distinct waves of primitive and definitive stromal progenitors during bone marrow development
AU - Mizoguchi, Toshihide
AU - Pinho, Sandra
AU - Ahmed, Jalal
AU - Kunisaki, Yuya
AU - Hanoun, Maher
AU - Mendelson, Avital
AU - Ono, Noriaki
AU - Kronenberg, Henry M.
AU - Frenette, Paul S.
N1 - Funding Information:
We thank D. Rowe, J. Butler, and S. Rafii for providing Col1(2.3)-Gfp mice. We would like to acknowledge C. Prophete, P. Ciero, L. Schiff, and A. Zahalka for technical assistance; L. Tesfa and O. Uche in the Einstein Flow Cytometry Core Facility for technical assistance of cell sorting; and P. Guo in the Analytical Imaging Facility for technical assistance in confocal microscope imaging. S.P. is a New York Stem Cell Foundation-Druckenmiller Fellow, and M.H. is a German Research Foundation (DFG) Fellow. This work was supported by The New York Stem Cell Foundation and by R01 grants from National Institutes of Health (DE022564 to N.O., DK056246 to H.M.K., and DK056638 and HL069438 to P.S.F.).
PY - 2014/5/12
Y1 - 2014/5/12
N2 - Mesenchymal stem and progenitor cells (MSPCs) contribute to bone marrow (BM) homeostasis by generating multiple types of stromal cells. MSPCs can be labeled in the adult BM by Nestin-GFP, whereas committed osteoblast progenitors are marked by Osterix expression. However, the developmental origin and hierarchical relationship of stromal cells remain largely unknown. Here, by using a lineage-tracing system, we describe three distinct waves of contributions of Osterix+ cells in the BM. First, Osterix+ progenitors in the fetal BM contribute to nascent bone tissues and transient stromal cells that are replaced in the adult marrow. Second, Osterix-expressing cells perinatally contribute to osteolineages and long-lived BM stroma, which have characteristics of Nestin-GFP+ MSPCs. Third, Osterix labeling in the adult marrow is osteolineage-restricted, devoid of stromal contribution. These results uncover a broad expression profile of Osterix and raise the intriguing possibility that distinct waves of stromal cells, primitive and definitive, may organize the developing BM.
AB - Mesenchymal stem and progenitor cells (MSPCs) contribute to bone marrow (BM) homeostasis by generating multiple types of stromal cells. MSPCs can be labeled in the adult BM by Nestin-GFP, whereas committed osteoblast progenitors are marked by Osterix expression. However, the developmental origin and hierarchical relationship of stromal cells remain largely unknown. Here, by using a lineage-tracing system, we describe three distinct waves of contributions of Osterix+ cells in the BM. First, Osterix+ progenitors in the fetal BM contribute to nascent bone tissues and transient stromal cells that are replaced in the adult marrow. Second, Osterix-expressing cells perinatally contribute to osteolineages and long-lived BM stroma, which have characteristics of Nestin-GFP+ MSPCs. Third, Osterix labeling in the adult marrow is osteolineage-restricted, devoid of stromal contribution. These results uncover a broad expression profile of Osterix and raise the intriguing possibility that distinct waves of stromal cells, primitive and definitive, may organize the developing BM.
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U2 - 10.1016/j.devcel.2014.03.013
DO - 10.1016/j.devcel.2014.03.013
M3 - Article
C2 - 24823377
AN - SCOPUS:84900309835
SN - 1534-5807
VL - 29
SP - 340
EP - 349
JO - Developmental cell
JF - Developmental cell
IS - 3
ER -