Osterix marks distinct waves of primitive and definitive stromal progenitors during bone marrow development

Toshihide Mizoguchi, Sandra I. Pinho, Jalal Ahmed, Yuya Kunisaki, Maher Hanoun, Avital Mendelson, Noriaki Ono, Henry M. Kronenberg, Paul S. Frenette

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

Mesenchymal stem and progenitor cells (MSPCs) contribute to bone marrow (BM) homeostasis by generating multiple types of stromal cells. MSPCs can be labeled in the adult BM by Nestin-GFP, whereas committed osteoblast progenitors are marked by Osterix expression. However, the developmental origin and hierarchical relationship of stromal cells remain largely unknown. Here, by using a lineage-tracing system, we describe three distinct waves of contributions of Osterix+ cells in the BM. First, Osterix+ progenitors in the fetal BM contribute to nascent bone tissues and transient stromal cells that are replaced in the adult marrow. Second, Osterix-expressing cells perinatally contribute to osteolineages and long-lived BM stroma, which have characteristics of Nestin-GFP+ MSPCs. Third, Osterix labeling in the adult marrow is osteolineage-restricted, devoid of stromal contribution. These results uncover a broad expression profile of Osterix and raise the intriguing possibility that distinct waves of stromal cells, primitive and definitive, may organize the developing BM.

Original languageEnglish (US)
Pages (from-to)340-349
Number of pages10
JournalDevelopmental Cell
Volume29
Issue number3
DOIs
StatePublished - May 12 2014

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Bone Development
Mesenchymal Stromal Cells
Bone
Bone Marrow
Stromal Cells
Nestin
Osteoblasts
Bone Marrow Cells
Labeling
Homeostasis
Tissue
Bone and Bones

ASJC Scopus subject areas

  • Developmental Biology

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Osterix marks distinct waves of primitive and definitive stromal progenitors during bone marrow development. / Mizoguchi, Toshihide; Pinho, Sandra I.; Ahmed, Jalal; Kunisaki, Yuya; Hanoun, Maher; Mendelson, Avital; Ono, Noriaki; Kronenberg, Henry M.; Frenette, Paul S.

In: Developmental Cell, Vol. 29, No. 3, 12.05.2014, p. 340-349.

Research output: Contribution to journalArticle

Mizoguchi, Toshihide ; Pinho, Sandra I. ; Ahmed, Jalal ; Kunisaki, Yuya ; Hanoun, Maher ; Mendelson, Avital ; Ono, Noriaki ; Kronenberg, Henry M. ; Frenette, Paul S. / Osterix marks distinct waves of primitive and definitive stromal progenitors during bone marrow development. In: Developmental Cell. 2014 ; Vol. 29, No. 3. pp. 340-349.
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