TY - JOUR
T1 - Origin of malondialdehyde from DNA degraded by Fe(II)-bleomycin
AU - Burger, R. M.
AU - Berkowitz, A. R.
AU - Peisach, J.
AU - Horwitz, S. B.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1980
Y1 - 1980
N2 - Ferrous bleomycin is known to break DNA efficiently in vitro in the presence of O2, giving rise to oligonucleotides, bases, and compounds resembling malondialdehyde in their chromogenic reaction with 2-thiobarbituric acid. Chromatography of radiolabeled DNA reaction mixtures resolves three kinds of malondialdehyde-like products, related by sequential conversions. The first chromogenic product is linked to DNA, and its formation does not entail the release of a base. It decomposes readily to the second product, a compound containing the base and deoxyribose carbons 1'-3'. Hydrolysis of either product yields the third, which is indistinguishable from authentic malondialdehyde. These findings suggest that the oxygen-dependent cleavage of DNA of Fe(II)·bleomycin can begin with the rupture of the deoxyribose 3'-4'-carbon bond. The initiation of these events is concurrent with the initiation of another mode of DNA degradation, involving the release of free base alone, in a yield similar to that of chromogen.
AB - Ferrous bleomycin is known to break DNA efficiently in vitro in the presence of O2, giving rise to oligonucleotides, bases, and compounds resembling malondialdehyde in their chromogenic reaction with 2-thiobarbituric acid. Chromatography of radiolabeled DNA reaction mixtures resolves three kinds of malondialdehyde-like products, related by sequential conversions. The first chromogenic product is linked to DNA, and its formation does not entail the release of a base. It decomposes readily to the second product, a compound containing the base and deoxyribose carbons 1'-3'. Hydrolysis of either product yields the third, which is indistinguishable from authentic malondialdehyde. These findings suggest that the oxygen-dependent cleavage of DNA of Fe(II)·bleomycin can begin with the rupture of the deoxyribose 3'-4'-carbon bond. The initiation of these events is concurrent with the initiation of another mode of DNA degradation, involving the release of free base alone, in a yield similar to that of chromogen.
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M3 - Article
C2 - 6160153
AN - SCOPUS:0019254695
SN - 0021-9258
VL - 255
SP - 11832
EP - 11838
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 24
ER -