Origin of malondialdehyde from DNA degraded by Fe(II)-bleomycin

R. M. Burger, A. R. Berkowitz, J. Peisach, S. B. Horwitz

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Abstract

Ferrous bleomycin is known to break DNA efficiently in vitro in the presence of O2, giving rise to oligonucleotides, bases, and compounds resembling malondialdehyde in their chromogenic reaction with 2-thiobarbituric acid. Chromatography of radiolabeled DNA reaction mixtures resolves three kinds of malondialdehyde-like products, related by sequential conversions. The first chromogenic product is linked to DNA, and its formation does not entail the release of a base. It decomposes readily to the second product, a compound containing the base and deoxyribose carbons 1'-3'. Hydrolysis of either product yields the third, which is indistinguishable from authentic malondialdehyde. These findings suggest that the oxygen-dependent cleavage of DNA of Fe(II)·bleomycin can begin with the rupture of the deoxyribose 3'-4'-carbon bond. The initiation of these events is concurrent with the initiation of another mode of DNA degradation, involving the release of free base alone, in a yield similar to that of chromogen.

Original languageEnglish (US)
Pages (from-to)11832-11838
Number of pages7
JournalJournal of Biological Chemistry
Volume255
Issue number24
Publication statusPublished - Dec 1 1980

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Burger, R. M., Berkowitz, A. R., Peisach, J., & Horwitz, S. B. (1980). Origin of malondialdehyde from DNA degraded by Fe(II)-bleomycin. Journal of Biological Chemistry, 255(24), 11832-11838.