Origin of malondialdehyde from DNA degraded by Fe(II)-bleomycin

R. M. Burger, A. R. Berkowitz, J. Peisach, Susan Band Horwitz

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

Ferrous bleomycin is known to break DNA efficiently in vitro in the presence of O2, giving rise to oligonucleotides, bases, and compounds resembling malondialdehyde in their chromogenic reaction with 2-thiobarbituric acid. Chromatography of radiolabeled DNA reaction mixtures resolves three kinds of malondialdehyde-like products, related by sequential conversions. The first chromogenic product is linked to DNA, and its formation does not entail the release of a base. It decomposes readily to the second product, a compound containing the base and deoxyribose carbons 1'-3'. Hydrolysis of either product yields the third, which is indistinguishable from authentic malondialdehyde. These findings suggest that the oxygen-dependent cleavage of DNA of Fe(II)·bleomycin can begin with the rupture of the deoxyribose 3'-4'-carbon bond. The initiation of these events is concurrent with the initiation of another mode of DNA degradation, involving the release of free base alone, in a yield similar to that of chromogen.

Original languageEnglish (US)
Pages (from-to)11832-11838
Number of pages7
JournalJournal of Biological Chemistry
Volume255
Issue number24
StatePublished - 1980

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Malondialdehyde
Chromogenics
DNA
Carbon
Deoxyribose
DNA Cleavage
DNA Breaks
Bleomycin
Oligonucleotides
Chromatography
Rupture
Hydrolysis
Oxygen
iron bleomycin
Degradation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Burger, R. M., Berkowitz, A. R., Peisach, J., & Band Horwitz, S. (1980). Origin of malondialdehyde from DNA degraded by Fe(II)-bleomycin. Journal of Biological Chemistry, 255(24), 11832-11838.

Origin of malondialdehyde from DNA degraded by Fe(II)-bleomycin. / Burger, R. M.; Berkowitz, A. R.; Peisach, J.; Band Horwitz, Susan.

In: Journal of Biological Chemistry, Vol. 255, No. 24, 1980, p. 11832-11838.

Research output: Contribution to journalArticle

Burger, RM, Berkowitz, AR, Peisach, J & Band Horwitz, S 1980, 'Origin of malondialdehyde from DNA degraded by Fe(II)-bleomycin', Journal of Biological Chemistry, vol. 255, no. 24, pp. 11832-11838.
Burger, R. M. ; Berkowitz, A. R. ; Peisach, J. ; Band Horwitz, Susan. / Origin of malondialdehyde from DNA degraded by Fe(II)-bleomycin. In: Journal of Biological Chemistry. 1980 ; Vol. 255, No. 24. pp. 11832-11838.
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