Organelle relationships in cultured 3T3-L1 preadipocytes.

A. B. Novikoff, P. M. Novikoff, O. M. Rosen, C. S. Rubin

Research output: Contribution to journalArticle

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Abstract

In differentiating 3T3-L1 cells, lipid spheres, the endoplasmic reticulum (ER), microperoxisomes, and mitochondria form "constellations" that may reflect the interplay of lipid metabolizing enzymes in these organelles. ER cisternae are also situated very close to "rosettes,"plasmalemmal specializations found in mature adipocytes in vivo. As in hepatocytes and absorptive cells of the intestine, this spatial relationship of ER and plasmalemma suggests a role for rosettes in the uptake of exogenous lipid precursors. The morphological differentiation of 3T3-L1 preadipocytes includes the loss of "stress fibers" and the appearance of microfilament like structures that encase, in a complex manner, the cytosolic lipid spheres that appear during differentiation. Other features described for the first time in 3T3-L1 preadipocytes include: (a) the presence of an extensive acid phosphatase (AcPase) positive GERL from which coated vesicles apparently arise (these coated vesicles display AcPase activity and are much smaller and far more numerous than the coated vesicles that seem to arise from the plasmalemmal coated pits); (b) the abundance of AcPase-positive autophagic vacuoles; and (c) a high level of alpha-naphthyl-acetate-esterase activity which, by light microscopy cytochemistry, appears to be localized in the cytosol.

Original languageEnglish (US)
Pages (from-to)180-196
Number of pages17
JournalJournal of Cell Biology
Volume87
Issue number1
StatePublished - Oct 1980

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Coated Vesicles
Organelles
Acid Phosphatase
Endoplasmic Reticulum
Lipids
Naphthol AS D Esterase
3T3-L1 Cells
Histocytochemistry
Stress Fibers
Vacuoles
Actin Cytoskeleton
Adipocytes
Cytosol
Intestines
Microscopy
Hepatocytes
Mitochondria
Light
Enzymes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Novikoff, A. B., Novikoff, P. M., Rosen, O. M., & Rubin, C. S. (1980). Organelle relationships in cultured 3T3-L1 preadipocytes. Journal of Cell Biology, 87(1), 180-196.

Organelle relationships in cultured 3T3-L1 preadipocytes. / Novikoff, A. B.; Novikoff, P. M.; Rosen, O. M.; Rubin, C. S.

In: Journal of Cell Biology, Vol. 87, No. 1, 10.1980, p. 180-196.

Research output: Contribution to journalArticle

Novikoff, AB, Novikoff, PM, Rosen, OM & Rubin, CS 1980, 'Organelle relationships in cultured 3T3-L1 preadipocytes.', Journal of Cell Biology, vol. 87, no. 1, pp. 180-196.
Novikoff AB, Novikoff PM, Rosen OM, Rubin CS. Organelle relationships in cultured 3T3-L1 preadipocytes. Journal of Cell Biology. 1980 Oct;87(1):180-196.
Novikoff, A. B. ; Novikoff, P. M. ; Rosen, O. M. ; Rubin, C. S. / Organelle relationships in cultured 3T3-L1 preadipocytes. In: Journal of Cell Biology. 1980 ; Vol. 87, No. 1. pp. 180-196.
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