Optogenetics: Rho GTPases activated by light in living macrophages

Maren Hülsemann, Polina V. Verkhusha, Peng Guo, Veronika Miskolci, Dianne Cox, Louis Hodgson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Genetically encoded optogenetic tools are increasingly popular and useful for perturbing signaling pathways with high spatial and temporal resolution in living cells. Here, we show basic procedures employed to implement optogenetics of Rho GTPases in a macrophage cell line. Methods described here are generally applicable to other genetically encoded optogenetic tools utilizing the blue-green spectrum of light for activation, designed for specific proteins and enzymatic targets important for immune cell functions.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages281-293
Number of pages13
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2108
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fingerprint

Optogenetics
rho GTP-Binding Proteins
Macrophages
Light
Methyl Green
Cell Line
Proteins

Keywords

  • LOV2
  • Optogenetics
  • Photoactivatable proteins
  • Rho GTPases

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Hülsemann, M., Verkhusha, P. V., Guo, P., Miskolci, V., Cox, D., & Hodgson, L. (2020). Optogenetics: Rho GTPases activated by light in living macrophages. In Methods in Molecular Biology (pp. 281-293). (Methods in Molecular Biology; Vol. 2108). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0247-8_24

Optogenetics : Rho GTPases activated by light in living macrophages. / Hülsemann, Maren; Verkhusha, Polina V.; Guo, Peng; Miskolci, Veronika; Cox, Dianne; Hodgson, Louis.

Methods in Molecular Biology. Humana Press Inc., 2020. p. 281-293 (Methods in Molecular Biology; Vol. 2108).

Research output: Chapter in Book/Report/Conference proceedingChapter

Hülsemann, M, Verkhusha, PV, Guo, P, Miskolci, V, Cox, D & Hodgson, L 2020, Optogenetics: Rho GTPases activated by light in living macrophages. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2108, Humana Press Inc., pp. 281-293. https://doi.org/10.1007/978-1-0716-0247-8_24
Hülsemann M, Verkhusha PV, Guo P, Miskolci V, Cox D, Hodgson L. Optogenetics: Rho GTPases activated by light in living macrophages. In Methods in Molecular Biology. Humana Press Inc. 2020. p. 281-293. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-0716-0247-8_24
Hülsemann, Maren ; Verkhusha, Polina V. ; Guo, Peng ; Miskolci, Veronika ; Cox, Dianne ; Hodgson, Louis. / Optogenetics : Rho GTPases activated by light in living macrophages. Methods in Molecular Biology. Humana Press Inc., 2020. pp. 281-293 (Methods in Molecular Biology).
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