@inbook{962e3240021c4bc7ac48f73c2591313d,
title = "Optogenetics: Rho GTPases activated by light in living macrophages",
abstract = "Genetically encoded optogenetic tools are increasingly popular and useful for perturbing signaling pathways with high spatial and temporal resolution in living cells. Here, we show basic procedures employed to implement optogenetics of Rho GTPases in a macrophage cell line. Methods described here are generally applicable to other genetically encoded optogenetic tools utilizing the blue-green spectrum of light for activation, designed for specific proteins and enzymatic targets important for immune cell functions.",
keywords = "LOV2, Optogenetics, Photoactivatable proteins, Rho GTPases",
author = "Maren H{\"u}lsemann and Verkhusha, {Polina V.} and Peng Guo and Veronika Miskolci and Dianne Cox and Louis Hodgson",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2020.",
year = "2020",
doi = "10.1007/978-1-0716-0247-8_24",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "281--293",
booktitle = "Methods in Molecular Biology",
}