Optogenetics: Rho GTPases activated by light in living macrophages

Maren Hülsemann, Polina V. Verkhusha, Peng Guo, Veronika Miskolci, Dianne Cox, Louis Hodgson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Genetically encoded optogenetic tools are increasingly popular and useful for perturbing signaling pathways with high spatial and temporal resolution in living cells. Here, we show basic procedures employed to implement optogenetics of Rho GTPases in a macrophage cell line. Methods described here are generally applicable to other genetically encoded optogenetic tools utilizing the blue-green spectrum of light for activation, designed for specific proteins and enzymatic targets important for immune cell functions.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages281-293
Number of pages13
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2108
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • LOV2
  • Optogenetics
  • Photoactivatable proteins
  • Rho GTPases

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Hülsemann, M., Verkhusha, P. V., Guo, P., Miskolci, V., Cox, D., & Hodgson, L. (2020). Optogenetics: Rho GTPases activated by light in living macrophages. In Methods in Molecular Biology (pp. 281-293). (Methods in Molecular Biology; Vol. 2108). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0247-8_24