Optimized release matrices for use in a BioMEMS device to study metastasis

Ashley Clark, James Williams, Michael Padgen, Patricia Keely, John S. Condeelis, James Castracane

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Multiple changes within the tumor microenvironment have been correlated with an increase in metastasis, yet the mechanisms are not fully understood. Tumor cells can be stimulated by the release of chemoattractant factors such as epidermal growth factor (EGF) from nearby stromal cells, resulting in increased intravasation and metastasis. Additionally, altered extracellular matrix density can result in changes in gene expression patterns governing increased cellular proliferation and motility. The Nano Intravital Device (NANIVID) has been used to produce gradients of select soluble factors in the tumor microenvironment and to study the role of these changes on cell migration. In previous studies, the NANIVID utilized a synthetic hydrogel to produce an EGF gradient to attract metastatic breast cancer cells. In this work, a matrigel insert will be introduced into the outlet to provide a substrate for cells to migrate on when entering the device. The concentration of the chemoattractant and matrigel comprising the insert will be optimized to produce a suitable gradient for inducing chemotaxis in metastatic breast cancer cells in vitro. Additionally, silk and alginate matrices will be explored as improved soluble factor release mediums. Delivery of larger molecules such as collagen cross-linkers requires an alternative hydrogel material. Future NANIVID experiments will utilize these materials to gauge the cellular motility response when a stiffer matrix is encountered.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Volume8615
DOIs
StatePublished - 2013
EventMicrofluidics, BioMEMS, and Medical Microsystems XI - San Francisco, CA, United States
Duration: Feb 3 2013Feb 5 2013

Other

OtherMicrofluidics, BioMEMS, and Medical Microsystems XI
CountryUnited States
CitySan Francisco, CA
Period2/3/132/5/13

Fingerprint

BioMEMS
Metastasis
metastasis
Tumors
Hydrogel
Chemotactic Factors
Cells
Epidermal Growth Factor
Hydrogels
Cell
matrices
Tumor
Motility
tumors
locomotion
Growth Factors
inserts
Gradient
Breast Cancer
Silk

Keywords

  • alginate
  • hydrogel
  • metastasis
  • silk
  • tumor microenvironment

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Clark, A., Williams, J., Padgen, M., Keely, P., Condeelis, J. S., & Castracane, J. (2013). Optimized release matrices for use in a BioMEMS device to study metastasis. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 8615). [86150C] https://doi.org/10.1117/12.2005048

Optimized release matrices for use in a BioMEMS device to study metastasis. / Clark, Ashley; Williams, James; Padgen, Michael; Keely, Patricia; Condeelis, John S.; Castracane, James.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 8615 2013. 86150C.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Clark, A, Williams, J, Padgen, M, Keely, P, Condeelis, JS & Castracane, J 2013, Optimized release matrices for use in a BioMEMS device to study metastasis. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 8615, 86150C, Microfluidics, BioMEMS, and Medical Microsystems XI, San Francisco, CA, United States, 2/3/13. https://doi.org/10.1117/12.2005048
Clark A, Williams J, Padgen M, Keely P, Condeelis JS, Castracane J. Optimized release matrices for use in a BioMEMS device to study metastasis. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 8615. 2013. 86150C https://doi.org/10.1117/12.2005048
Clark, Ashley ; Williams, James ; Padgen, Michael ; Keely, Patricia ; Condeelis, John S. ; Castracane, James. / Optimized release matrices for use in a BioMEMS device to study metastasis. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 8615 2013.
@inproceedings{b9183df25ac44ec1af6ac9d62d8ccef0,
title = "Optimized release matrices for use in a BioMEMS device to study metastasis",
abstract = "Multiple changes within the tumor microenvironment have been correlated with an increase in metastasis, yet the mechanisms are not fully understood. Tumor cells can be stimulated by the release of chemoattractant factors such as epidermal growth factor (EGF) from nearby stromal cells, resulting in increased intravasation and metastasis. Additionally, altered extracellular matrix density can result in changes in gene expression patterns governing increased cellular proliferation and motility. The Nano Intravital Device (NANIVID) has been used to produce gradients of select soluble factors in the tumor microenvironment and to study the role of these changes on cell migration. In previous studies, the NANIVID utilized a synthetic hydrogel to produce an EGF gradient to attract metastatic breast cancer cells. In this work, a matrigel insert will be introduced into the outlet to provide a substrate for cells to migrate on when entering the device. The concentration of the chemoattractant and matrigel comprising the insert will be optimized to produce a suitable gradient for inducing chemotaxis in metastatic breast cancer cells in vitro. Additionally, silk and alginate matrices will be explored as improved soluble factor release mediums. Delivery of larger molecules such as collagen cross-linkers requires an alternative hydrogel material. Future NANIVID experiments will utilize these materials to gauge the cellular motility response when a stiffer matrix is encountered.",
keywords = "alginate, hydrogel, metastasis, silk, tumor microenvironment",
author = "Ashley Clark and James Williams and Michael Padgen and Patricia Keely and Condeelis, {John S.} and James Castracane",
year = "2013",
doi = "10.1117/12.2005048",
language = "English (US)",
isbn = "9780819493842",
volume = "8615",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

}

TY - GEN

T1 - Optimized release matrices for use in a BioMEMS device to study metastasis

AU - Clark, Ashley

AU - Williams, James

AU - Padgen, Michael

AU - Keely, Patricia

AU - Condeelis, John S.

AU - Castracane, James

PY - 2013

Y1 - 2013

N2 - Multiple changes within the tumor microenvironment have been correlated with an increase in metastasis, yet the mechanisms are not fully understood. Tumor cells can be stimulated by the release of chemoattractant factors such as epidermal growth factor (EGF) from nearby stromal cells, resulting in increased intravasation and metastasis. Additionally, altered extracellular matrix density can result in changes in gene expression patterns governing increased cellular proliferation and motility. The Nano Intravital Device (NANIVID) has been used to produce gradients of select soluble factors in the tumor microenvironment and to study the role of these changes on cell migration. In previous studies, the NANIVID utilized a synthetic hydrogel to produce an EGF gradient to attract metastatic breast cancer cells. In this work, a matrigel insert will be introduced into the outlet to provide a substrate for cells to migrate on when entering the device. The concentration of the chemoattractant and matrigel comprising the insert will be optimized to produce a suitable gradient for inducing chemotaxis in metastatic breast cancer cells in vitro. Additionally, silk and alginate matrices will be explored as improved soluble factor release mediums. Delivery of larger molecules such as collagen cross-linkers requires an alternative hydrogel material. Future NANIVID experiments will utilize these materials to gauge the cellular motility response when a stiffer matrix is encountered.

AB - Multiple changes within the tumor microenvironment have been correlated with an increase in metastasis, yet the mechanisms are not fully understood. Tumor cells can be stimulated by the release of chemoattractant factors such as epidermal growth factor (EGF) from nearby stromal cells, resulting in increased intravasation and metastasis. Additionally, altered extracellular matrix density can result in changes in gene expression patterns governing increased cellular proliferation and motility. The Nano Intravital Device (NANIVID) has been used to produce gradients of select soluble factors in the tumor microenvironment and to study the role of these changes on cell migration. In previous studies, the NANIVID utilized a synthetic hydrogel to produce an EGF gradient to attract metastatic breast cancer cells. In this work, a matrigel insert will be introduced into the outlet to provide a substrate for cells to migrate on when entering the device. The concentration of the chemoattractant and matrigel comprising the insert will be optimized to produce a suitable gradient for inducing chemotaxis in metastatic breast cancer cells in vitro. Additionally, silk and alginate matrices will be explored as improved soluble factor release mediums. Delivery of larger molecules such as collagen cross-linkers requires an alternative hydrogel material. Future NANIVID experiments will utilize these materials to gauge the cellular motility response when a stiffer matrix is encountered.

KW - alginate

KW - hydrogel

KW - metastasis

KW - silk

KW - tumor microenvironment

UR - http://www.scopus.com/inward/record.url?scp=84878143185&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878143185&partnerID=8YFLogxK

U2 - 10.1117/12.2005048

DO - 10.1117/12.2005048

M3 - Conference contribution

SN - 9780819493842

VL - 8615

BT - Proceedings of SPIE - The International Society for Optical Engineering

ER -