Oncogene activation and surface markers in mouse lymphomas induced by radiation and nitrosomethylurea

I. Guerrero, A. Villasante, L. Diamond, J. W. Berman, E. W. Newcomb, J. J. Steinberg, R. Lake, A. Pellicer

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Thymic lymphomas have been induced by γ-radiation and treatment with the chemical nitrosomethylurea in different mice strains. As indicated by the NIH 3T3 focus forming assay, a significant percentage of the tumors contain activated oncogenes of the ras family (K or N). Cloning and sequencing has enabled us to identify single base mutations as the only significant alteration present in the activated oncogenes. These alterations result in the substitution of amino-acid 12 or 61 of the p21 product of the ras genes. With the use of synthetic oligonucleotides it has been found that the tumors do not all contain the same mutation and in one case so far the normal allele is absent.

Original languageEnglish (US)
Pages (from-to)851-858
Number of pages8
JournalLeukemia Research
Volume10
Issue number7
DOIs
StatePublished - 1986

Keywords

  • N-ras
  • Oncogene
  • leukemia
  • radiation

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Oncogene activation and surface markers in mouse lymphomas induced by radiation and nitrosomethylurea'. Together they form a unique fingerprint.

Cite this