Oncogene activation and surface markers in mouse lymphomas induced by radiation and nitrosomethylurea

I. Guerrero; A. Villasante; L. Diamond; J. W. Berman; E. W. Newcomb; J. J. Steinberg; R. Lake; A. Pellicer

doi:10.1016/0145-2126(86)90306-1

Oncogene activation and surface markers in mouse lymphomas induced by radiation and nitrosomethylurea

I. Guerrero, A. Villasante, L. Diamond, J. W. Berman, E. W. Newcomb, J. J. Steinberg, R. Lake, A. Pellicer

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Thymic lymphomas have been induced by γ-radiation and treatment with the chemical nitrosomethylurea in different mice strains. As indicated by the NIH 3T3 focus forming assay, a significant percentage of the tumors contain activated oncogenes of the ras family (K or N). Cloning and sequencing has enabled us to identify single base mutations as the only significant alteration present in the activated oncogenes. These alterations result in the substitution of amino-acid 12 or 61 of the p21 product of the ras genes. With the use of synthetic oligonucleotides it has been found that the tumors do not all contain the same mutation and in one case so far the normal allele is absent.

Original language	English (US)
Pages (from-to)	851-858
Number of pages	8
Journal	Leukemia Research
Volume	10
Issue number	7
DOIs	https://doi.org/10.1016/0145-2126(86)90306-1
State	Published - 1986
Externally published	Yes

Keywords

N-ras
Oncogene
leukemia
radiation

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/0145-2126(86)90306-1

Cite this

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title = "Oncogene activation and surface markers in mouse lymphomas induced by radiation and nitrosomethylurea",

abstract = "Thymic lymphomas have been induced by γ-radiation and treatment with the chemical nitrosomethylurea in different mice strains. As indicated by the NIH 3T3 focus forming assay, a significant percentage of the tumors contain activated oncogenes of the ras family (K or N). Cloning and sequencing has enabled us to identify single base mutations as the only significant alteration present in the activated oncogenes. These alterations result in the substitution of amino-acid 12 or 61 of the p21 product of the ras genes. With the use of synthetic oligonucleotides it has been found that the tumors do not all contain the same mutation and in one case so far the normal allele is absent.",

keywords = "N-ras, Oncogene, leukemia, radiation",

author = "I. Guerrero and A. Villasante and L. Diamond and Berman, {J. W.} and Newcomb, {E. W.} and Steinberg, {J. J.} and R. Lake and A. Pellicer",

year = "1986",

doi = "10.1016/0145-2126(86)90306-1",

language = "English (US)",

volume = "10",

pages = "851--858",

journal = "Leukemia Research",

issn = "0145-2126",

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TY - JOUR

T1 - Oncogene activation and surface markers in mouse lymphomas induced by radiation and nitrosomethylurea

AU - Guerrero, I.

AU - Villasante, A.

AU - Diamond, L.

AU - Berman, J. W.

AU - Newcomb, E. W.

AU - Steinberg, J. J.

AU - Lake, R.

AU - Pellicer, A.

PY - 1986

Y1 - 1986

N2 - Thymic lymphomas have been induced by γ-radiation and treatment with the chemical nitrosomethylurea in different mice strains. As indicated by the NIH 3T3 focus forming assay, a significant percentage of the tumors contain activated oncogenes of the ras family (K or N). Cloning and sequencing has enabled us to identify single base mutations as the only significant alteration present in the activated oncogenes. These alterations result in the substitution of amino-acid 12 or 61 of the p21 product of the ras genes. With the use of synthetic oligonucleotides it has been found that the tumors do not all contain the same mutation and in one case so far the normal allele is absent.

AB - Thymic lymphomas have been induced by γ-radiation and treatment with the chemical nitrosomethylurea in different mice strains. As indicated by the NIH 3T3 focus forming assay, a significant percentage of the tumors contain activated oncogenes of the ras family (K or N). Cloning and sequencing has enabled us to identify single base mutations as the only significant alteration present in the activated oncogenes. These alterations result in the substitution of amino-acid 12 or 61 of the p21 product of the ras genes. With the use of synthetic oligonucleotides it has been found that the tumors do not all contain the same mutation and in one case so far the normal allele is absent.

KW - N-ras

KW - Oncogene

KW - leukemia

KW - radiation

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DO - 10.1016/0145-2126(86)90306-1

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JO - Leukemia Research

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Oncogene activation and surface markers in mouse lymphomas induced by radiation and nitrosomethylurea

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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