Nutritional conditions modulate c. Neoformans extracellular vesicles’ capacity to elicit host immune response

Clara Luna Marina, Pedro Henrique Bürgel, Daniel Paiva Agostinho, Daniel Zamith-Miranda, Lucas de Oliveira Las-Casas, Aldo Henrique Tavares, Joshua Daniel Nosanchuk, Anamelia Lorenzetti Bocca

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Cryptococcus neoformans is a human pathogenic fungus that mainly afflicts immunocompromised patients. One of its virulence strategies is the production of extracellular vesicles (EVs), containing cargo with immunomodulatory properties. We evaluated EV’s characteristics produced by capsular and acapsular strains of C. neoformans (B3501 and ∆Cap67, respectively) growing in nutritionally poor or rich media and co-cultures with bone marrow-derived macrophages or dendritic cells from C57BL/6 mice. EVs produced under a poor nutritional condition displayed a larger hydrodynamic size, contained more virulence compounds, and induced a more robust inflammatory pattern than those produced in a rich nutritional medium, independently of strain. We treated infected mice with EVs produced in the rich medium, and the EVs inhibited more genes related to the inflammasome than untreated infected mice. These findings suggest that the EVs participate in the pathogenic processes that result in the dissemination of C. neoformans. Thus, these results highlight the versatility of EVs’ properties during infection by C. neoformans in different tissues and support ongoing efforts to harness EVs to prevent and treat cryptococcosis.

Original languageEnglish (US)
Article number1815
Pages (from-to)1-17
Number of pages17
JournalMicroorganisms
Volume8
Issue number11
DOIs
StatePublished - Nov 2020

Keywords

  • Cryptococcus neoformans
  • Extracellular vesicles
  • Inflammasome

ASJC Scopus subject areas

  • Microbiology
  • Virology
  • Microbiology (medical)

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