Normal human dermis contains distinct populations of CD11c +BDCA-1+ dendritic cells and CD163+FXIIIA + macrophages

Lisa C. Zaba, Judilyn Fuentes-Duculan, Ralph M. Steinman, James G. Krueger, Michelle A. Lowes

Research output: Contribution to journalArticle

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Abstract

We used a panel of monoclonal antibodies to characterize DCs in the dermis of normal human skin. Staining for the CD11c integrin, which is abundant on many kinds of DCs, revealed cells in the upper dermis. These cells were positive for blood DC antigen-1 (BDCA-1; also known as CD1c), HLA-DR, and CD45, markers that are also expressed by circulating myeloid DCs. A small subset of CD11c + dermal cells expressed DEC-205/CD205 and DC-lysosomal-associated membrane glycoprotein/CD208 (DC-LAMP/CD208), suggesting some differentiation or maturation. When BDCA-1+ cells were selected from collagenase digests of normal dermis, they proved to be strong stimulators for T cells in a mixed leukocyte reaction. A second major population of cells located throughout the dermis was positive for factor XIIIA (FXIIIA), but lacked CD11c and BDCA-1. They expressed the macrophage scavenger receptor CD163 and stained weakly for HLA-DR and CD45. Isolated CD163+ dermal cells were inactive in stimulating T cell proliferation, but in biopsies of tattoos, these cells were selectively laden with granular pigments. Plasmacytoid DCs were also present in the dermis, marked by CD123 and BDCA-2. In summary, the normal dermis contains typical immunostimulatory myeloid DCs identified by CD11c and BDCA-1, as well as an additional population of poorly stimulatory macrophages marked by CD163 and FXIIIA.

Original languageEnglish (US)
Pages (from-to)2517-2525
Number of pages9
JournalJournal of Clinical Investigation
Volume117
Issue number9
DOIs
StatePublished - Sep 4 2007
Externally publishedYes

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Dermis
Dendritic Cells
Macrophages
Population
HLA-DR Antigens
Skin
Lysosome-Associated Membrane Glycoproteins
T-Lymphocytes
Scavenger Receptors
Mixed Lymphocyte Culture Test
Collagenases
Integrins
Monoclonal Antibodies
Cell Proliferation
Staining and Labeling
Biopsy
Antigens

ASJC Scopus subject areas

  • Medicine(all)

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Normal human dermis contains distinct populations of CD11c +BDCA-1+ dendritic cells and CD163+FXIIIA + macrophages. / Zaba, Lisa C.; Fuentes-Duculan, Judilyn; Steinman, Ralph M.; Krueger, James G.; Lowes, Michelle A.

In: Journal of Clinical Investigation, Vol. 117, No. 9, 04.09.2007, p. 2517-2525.

Research output: Contribution to journalArticle

Zaba, Lisa C. ; Fuentes-Duculan, Judilyn ; Steinman, Ralph M. ; Krueger, James G. ; Lowes, Michelle A. / Normal human dermis contains distinct populations of CD11c +BDCA-1+ dendritic cells and CD163+FXIIIA + macrophages. In: Journal of Clinical Investigation. 2007 ; Vol. 117, No. 9. pp. 2517-2525.
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