Nonfermentative thermoalkaliphilic growth is restricted to alkaline environments

Duncan G G McMillan, Stefanie Keis, Michael Berney, Gregory M. Cook

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Caldalkalibacillus thermarum strain TA2.A1 grew in pH-controlled batch culture containing a fermentable growth substrate (i.e., sucrose) from pH 7.5 to 10.0 with no significant change in the specific growth rate, suggesting that this bacterium was a facultative alkaliphile. However, when strain TA2.A1 was grown on a nonfermentable carbon source, such as succinate or malate, no growth was observed until the external pH was >9.0, suggesting that this bacterium was an obligate alkaliphile. Succinate transport and sucrose transport by strain TA2.A1 showed pH profiles similar to that of growth on these carbon sources, and the molar growth yield on sucrose was higher at pH 9.5 than at pH 7.5, despite the increased energy demands on the cell for intracellular pH regulation. Succinate transport, succinate-dependent oxygen consumption, and succinate dehydrogenase and F1Fo-ATPase specific activities were all significantly lower in cultures of strain TA2.A1 grown at pH 7.5 than in those cultured at pH 9.5. No significant ATP synthesis via the F1Fo-ATP synthase was detected until the external pH was >8.5. On the basis of these results, we propose that nonfermentative thermoalkaliphilic growth is specialized to function at high pH values, but not at pH values near neutral pH.

Original languageEnglish (US)
Pages (from-to)7649-7654
Number of pages6
JournalApplied and Environmental Microbiology
Volume75
Issue number24
DOIs
StatePublished - Dec 2009
Externally publishedYes

Fingerprint

alkaline environment
sucrose
Growth
Succinic Acid
succinic acid
bacterium
carbon
Sucrose
oxygen consumption
succinate dehydrogenase (quinone)
substrate
Carbon
Adenosine Triphosphate
Bacteria
H-transporting ATP synthase
Batch Cell Culture Techniques
Succinate Dehydrogenase
bacteria
malates
Oxygen Consumption

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Nonfermentative thermoalkaliphilic growth is restricted to alkaline environments. / McMillan, Duncan G G; Keis, Stefanie; Berney, Michael; Cook, Gregory M.

In: Applied and Environmental Microbiology, Vol. 75, No. 24, 12.2009, p. 7649-7654.

Research output: Contribution to journalArticle

McMillan, Duncan G G ; Keis, Stefanie ; Berney, Michael ; Cook, Gregory M. / Nonfermentative thermoalkaliphilic growth is restricted to alkaline environments. In: Applied and Environmental Microbiology. 2009 ; Vol. 75, No. 24. pp. 7649-7654.
@article{a4005023843c4a3b85e34b95f49fca52,
title = "Nonfermentative thermoalkaliphilic growth is restricted to alkaline environments",
abstract = "Caldalkalibacillus thermarum strain TA2.A1 grew in pH-controlled batch culture containing a fermentable growth substrate (i.e., sucrose) from pH 7.5 to 10.0 with no significant change in the specific growth rate, suggesting that this bacterium was a facultative alkaliphile. However, when strain TA2.A1 was grown on a nonfermentable carbon source, such as succinate or malate, no growth was observed until the external pH was >9.0, suggesting that this bacterium was an obligate alkaliphile. Succinate transport and sucrose transport by strain TA2.A1 showed pH profiles similar to that of growth on these carbon sources, and the molar growth yield on sucrose was higher at pH 9.5 than at pH 7.5, despite the increased energy demands on the cell for intracellular pH regulation. Succinate transport, succinate-dependent oxygen consumption, and succinate dehydrogenase and F1Fo-ATPase specific activities were all significantly lower in cultures of strain TA2.A1 grown at pH 7.5 than in those cultured at pH 9.5. No significant ATP synthesis via the F1Fo-ATP synthase was detected until the external pH was >8.5. On the basis of these results, we propose that nonfermentative thermoalkaliphilic growth is specialized to function at high pH values, but not at pH values near neutral pH.",
author = "McMillan, {Duncan G G} and Stefanie Keis and Michael Berney and Cook, {Gregory M.}",
year = "2009",
month = "12",
doi = "10.1128/AEM.01639-09",
language = "English (US)",
volume = "75",
pages = "7649--7654",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "24",

}

TY - JOUR

T1 - Nonfermentative thermoalkaliphilic growth is restricted to alkaline environments

AU - McMillan, Duncan G G

AU - Keis, Stefanie

AU - Berney, Michael

AU - Cook, Gregory M.

PY - 2009/12

Y1 - 2009/12

N2 - Caldalkalibacillus thermarum strain TA2.A1 grew in pH-controlled batch culture containing a fermentable growth substrate (i.e., sucrose) from pH 7.5 to 10.0 with no significant change in the specific growth rate, suggesting that this bacterium was a facultative alkaliphile. However, when strain TA2.A1 was grown on a nonfermentable carbon source, such as succinate or malate, no growth was observed until the external pH was >9.0, suggesting that this bacterium was an obligate alkaliphile. Succinate transport and sucrose transport by strain TA2.A1 showed pH profiles similar to that of growth on these carbon sources, and the molar growth yield on sucrose was higher at pH 9.5 than at pH 7.5, despite the increased energy demands on the cell for intracellular pH regulation. Succinate transport, succinate-dependent oxygen consumption, and succinate dehydrogenase and F1Fo-ATPase specific activities were all significantly lower in cultures of strain TA2.A1 grown at pH 7.5 than in those cultured at pH 9.5. No significant ATP synthesis via the F1Fo-ATP synthase was detected until the external pH was >8.5. On the basis of these results, we propose that nonfermentative thermoalkaliphilic growth is specialized to function at high pH values, but not at pH values near neutral pH.

AB - Caldalkalibacillus thermarum strain TA2.A1 grew in pH-controlled batch culture containing a fermentable growth substrate (i.e., sucrose) from pH 7.5 to 10.0 with no significant change in the specific growth rate, suggesting that this bacterium was a facultative alkaliphile. However, when strain TA2.A1 was grown on a nonfermentable carbon source, such as succinate or malate, no growth was observed until the external pH was >9.0, suggesting that this bacterium was an obligate alkaliphile. Succinate transport and sucrose transport by strain TA2.A1 showed pH profiles similar to that of growth on these carbon sources, and the molar growth yield on sucrose was higher at pH 9.5 than at pH 7.5, despite the increased energy demands on the cell for intracellular pH regulation. Succinate transport, succinate-dependent oxygen consumption, and succinate dehydrogenase and F1Fo-ATPase specific activities were all significantly lower in cultures of strain TA2.A1 grown at pH 7.5 than in those cultured at pH 9.5. No significant ATP synthesis via the F1Fo-ATP synthase was detected until the external pH was >8.5. On the basis of these results, we propose that nonfermentative thermoalkaliphilic growth is specialized to function at high pH values, but not at pH values near neutral pH.

UR - http://www.scopus.com/inward/record.url?scp=73249152381&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=73249152381&partnerID=8YFLogxK

U2 - 10.1128/AEM.01639-09

DO - 10.1128/AEM.01639-09

M3 - Article

C2 - 19854920

AN - SCOPUS:73249152381

VL - 75

SP - 7649

EP - 7654

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 24

ER -