Nonenzymatic glycosylation products on collagen covalently trap low-density lipoprotein

M. Brownlee, H. Vlassara, A. Cerami

Research output: Contribution to journalArticle

172 Citations (Scopus)

Abstract

Advanced nonenzymatic glycosylation products capable of cross-linking proteins accumulate on collagen in vivo in proportion to time-averaged blood glucose concentration. In this report, we have evaluated the ability of advanced nonenzymatic glycosylation products formed on collagen in vitro to covalently bind low-density lipoprotein (LDL) in a manner similar to that which occurs in human atherosclerotic lesions. At constant LDL concentration, covalent trapping increased linearly with the extent of advanced glycosylation product formation, from 1.42 ± 0.15 to 4.46 ± 0.36 μg LDL protein/mg collagen. At a constant level of collagen advanced glycosylation product LDL binding increared as a function of increasing LDL concentration. At an LDL-cholesterol level of 103 mg/dl, covalent trapping of LDL by nonenzymatic glycosylation products on collagen averaged 3.2 times as much as control (P < 0.01). These data indicate that LDL is bound specifically by reactive products generated by nonenzymatic glycosylation of collagen, and suggest that excessive LDL trapping by hyperglycemia-induced advanced glycosylation endproducts may contribute to the accelerated development of atherosclerosis in patients with diabetes mellitus.

Original languageEnglish (US)
Pages (from-to)938-941
Number of pages4
JournalDiabetes
Volume34
Issue number9
StatePublished - 1985
Externally publishedYes

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Glycosylation
LDL Lipoproteins
Collagen
Hyperglycemia
LDL Cholesterol
Blood Glucose
Atherosclerosis
Diabetes Mellitus
Proteins

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Brownlee, M., Vlassara, H., & Cerami, A. (1985). Nonenzymatic glycosylation products on collagen covalently trap low-density lipoprotein. Diabetes, 34(9), 938-941.

Nonenzymatic glycosylation products on collagen covalently trap low-density lipoprotein. / Brownlee, M.; Vlassara, H.; Cerami, A.

In: Diabetes, Vol. 34, No. 9, 1985, p. 938-941.

Research output: Contribution to journalArticle

Brownlee, M, Vlassara, H & Cerami, A 1985, 'Nonenzymatic glycosylation products on collagen covalently trap low-density lipoprotein', Diabetes, vol. 34, no. 9, pp. 938-941.
Brownlee, M. ; Vlassara, H. ; Cerami, A. / Nonenzymatic glycosylation products on collagen covalently trap low-density lipoprotein. In: Diabetes. 1985 ; Vol. 34, No. 9. pp. 938-941.
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