Nitric oxide-releasing nanoparticles accelerate wound healing in NOD-SCID mice

Karin Blecher Paz, Luis R. Martinez, Chaim Tuckman-Vernon, Parimala Nacharaju, David Schairer, Jason Chouake, Joel M. Friedman, Alan Alfieri, Chandan Guha, Joshua D. Nosanchuk, Adam J. Friedman

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Wound healing is a complex process, coordinated by various biological factors. In immunocompromised states wound healing can be interrupted as a result of decreased numbers of immune cells, impairing the production of effector molecules such as nitric oxide (NO). Therefore, topical NO-releasing platforms, such as diethylenetriamine (DETA NONOate), have been investigated to enhance wound healing. Recently, we demonstrated a nanoparticle platform that releases NO (NO-NPs) in a sustained manner, accelerating wound healing in both uninfected and infected murine wound models. Here, NO-NPs were investigated and compared to DETA NONOate in an immunocompromised wound model using non-obese, diabetic, severe combined immunodeficiency mice. NO-NP treatment accelerated wound closure as compared to controls and DETA NONOate treatment. In addition, histological assessment revealed that wounds treated with NO-NPs had less inflammation, more collagen deposition, and more blood vessel formation as compared to other groups, consistent with our previous data in immunocompetent animals. These data suggest that NO-NPs may serve as a novel wound-healing therapy in the setting of immunocompromised states associated with impaired wound healing. From the Clinical Editor: Wound healing in an immunocompromised host is often incomplete and is a source of major concern in such conditions. This work demonstrates in a murine model that in these settings NO releasing nanoparticles significantly enhance wound healing.

Original languageEnglish (US)
Pages (from-to)1364-1371
Number of pages8
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume8
Issue number8
DOIs
StatePublished - Nov 2012

Fingerprint

Inbred NOD Mouse
SCID Mice
Nitric oxide
Wound Healing
Nanoparticles
Nitric Oxide
Wounds and Injuries
Severe Combined Immunodeficiency
Blood vessels
Biological Factors
Immunocompromised Host
Collagen
Blood Vessels
Animals
Cell Count
Inflammation
Molecules
2,2'-(hydroxynitrosohydrazono)bis-ethanamine

Keywords

  • Diazeniumdiolate
  • Immunodeficiency
  • Nanotechnology
  • Nitric oxide
  • Wound healing

ASJC Scopus subject areas

  • Molecular Medicine
  • Bioengineering
  • Biomedical Engineering
  • Materials Science(all)
  • Medicine (miscellaneous)
  • Pharmaceutical Science

Cite this

Nitric oxide-releasing nanoparticles accelerate wound healing in NOD-SCID mice. / Blecher Paz, Karin; Martinez, Luis R.; Tuckman-Vernon, Chaim; Nacharaju, Parimala; Schairer, David; Chouake, Jason; Friedman, Joel M.; Alfieri, Alan; Guha, Chandan; Nosanchuk, Joshua D.; Friedman, Adam J.

In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 8, No. 8, 11.2012, p. 1364-1371.

Research output: Contribution to journalArticle

Blecher Paz, Karin ; Martinez, Luis R. ; Tuckman-Vernon, Chaim ; Nacharaju, Parimala ; Schairer, David ; Chouake, Jason ; Friedman, Joel M. ; Alfieri, Alan ; Guha, Chandan ; Nosanchuk, Joshua D. ; Friedman, Adam J. / Nitric oxide-releasing nanoparticles accelerate wound healing in NOD-SCID mice. In: Nanomedicine: Nanotechnology, Biology, and Medicine. 2012 ; Vol. 8, No. 8. pp. 1364-1371.
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