New method of hepatocyte transplantation and extracorporeal liver support

A. A. Demetriou, J. Whiting, S. M. Levenson, N. R. Chowdhury, R. Schechner, S. Michalski, D. Feldman, J. R. Chowdhury

Research output: Contribution to journalArticle

124 Scopus citations

Abstract

A technique has been developed by the authors that allows hepatocyte attachment on collagen-coated microcarriers resulting in prolonged hepatocyte viability and function both in vivo and in vitro. Rat hepatocytes were obtained by portal vein collagenase perfusion. Intraperitoneally transplanted microcarrier-attached normal hepatocytes into congeneic Gunn rats were functioning 3-4 weeks later, as shown by the presence and persistence of conjugated bilirubin in recipient bile, sustained decrease in serum bilirubin, uptake of Tc99m-DESIDA, and morpholigic criteria. Intraperitoneal transplantation of normal microcarrier-attached hepatocytes into genetically albumin deficient rats (NAR) resulted in marked decrease in plasma albumin levels (6 days without and 21 days with Cyclosporin A immunosuppression). Microcarrier-attached hepatocytes transplanted after 2 weeks of storage at -80 C into congeneic Gunn rats were viable and functional as assessed by criteria outlined above. An extracorporeal liver perfusion system was developed using the microcarrier-attached hepatocytes that was capable of synthesizing and conjugating bilirubin and synthesizing liver-specific proteins.

Original languageEnglish (US)
Pages (from-to)259-271
Number of pages13
JournalAnnals of surgery
Volume204
Issue number3
DOIs
StatePublished - Jan 1 1986

ASJC Scopus subject areas

  • Surgery

Fingerprint Dive into the research topics of 'New method of hepatocyte transplantation and extracorporeal liver support'. Together they form a unique fingerprint.

  • Cite this

    Demetriou, A. A., Whiting, J., Levenson, S. M., Chowdhury, N. R., Schechner, R., Michalski, S., Feldman, D., & Chowdhury, J. R. (1986). New method of hepatocyte transplantation and extracorporeal liver support. Annals of surgery, 204(3), 259-271. https://doi.org/10.1097/00000658-198609000-00005