New method of hepatocyte transplantation and extracorporeal liver support

A. A. Demetriou, J. Whiting, S. M. Levenson, N. R. Chowdhury, R. Schechner, S. Michalski, D. Feldman, Jayanta Roy-Chowdhury

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

A technique has been developed by the authors that allows hepatocyte attachment on collagen-coated microcarriers resulting in prolonged hepatocyte viability and function both in vivo and in vitro. Rat hepatocytes were obtained by portal vein collagenase perfusion. Intraperitoneally transplanted microcarrier-attached normal hepatocytes into congeneic Gunn rats were functioning 3-4 weeks later, as shown by the presence and persistence of conjugated bilirubin in recipient bile, sustained decrease in serum bilirubin, uptake of Tc99m-DESIDA, and morpholigic criteria. Intraperitoneal transplantation of normal microcarrier-attached hepatocytes into genetically albumin deficient rats (NAR) resulted in marked decrease in plasma albumin levels (6 days without and 21 days with Cyclosporin A immunosuppression). Microcarrier-attached hepatocytes transplanted after 2 weeks of storage at -80 C into congeneic Gunn rats were viable and functional as assessed by criteria outlined above. An extracorporeal liver perfusion system was developed using the microcarrier-attached hepatocytes that was capable of synthesizing and conjugating bilirubin and synthesizing liver-specific proteins.

Original languageEnglish (US)
Pages (from-to)259-271
Number of pages13
JournalAnnals of Surgery
Volume204
Issue number3
StatePublished - 1986
Externally publishedYes

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Liver Transplantation
Hepatocytes
Gunn Rats
Bilirubin
Perfusion
Liver
Collagenases
Portal Vein
Bile
Serum Albumin
Immunosuppression
Cyclosporine
Albumins
Collagen
Transplantation
Serum
Proteins

ASJC Scopus subject areas

  • Surgery

Cite this

Demetriou, A. A., Whiting, J., Levenson, S. M., Chowdhury, N. R., Schechner, R., Michalski, S., ... Roy-Chowdhury, J. (1986). New method of hepatocyte transplantation and extracorporeal liver support. Annals of Surgery, 204(3), 259-271.

New method of hepatocyte transplantation and extracorporeal liver support. / Demetriou, A. A.; Whiting, J.; Levenson, S. M.; Chowdhury, N. R.; Schechner, R.; Michalski, S.; Feldman, D.; Roy-Chowdhury, Jayanta.

In: Annals of Surgery, Vol. 204, No. 3, 1986, p. 259-271.

Research output: Contribution to journalArticle

Demetriou, AA, Whiting, J, Levenson, SM, Chowdhury, NR, Schechner, R, Michalski, S, Feldman, D & Roy-Chowdhury, J 1986, 'New method of hepatocyte transplantation and extracorporeal liver support', Annals of Surgery, vol. 204, no. 3, pp. 259-271.
Demetriou AA, Whiting J, Levenson SM, Chowdhury NR, Schechner R, Michalski S et al. New method of hepatocyte transplantation and extracorporeal liver support. Annals of Surgery. 1986;204(3):259-271.
Demetriou, A. A. ; Whiting, J. ; Levenson, S. M. ; Chowdhury, N. R. ; Schechner, R. ; Michalski, S. ; Feldman, D. ; Roy-Chowdhury, Jayanta. / New method of hepatocyte transplantation and extracorporeal liver support. In: Annals of Surgery. 1986 ; Vol. 204, No. 3. pp. 259-271.
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