Neuroprotective effect of carvedilol and melatonin on 3-nitropropionic acid-induced neurotoxicity in neuroblastoma

Inmaculada Tasset, C. Espínola, F. J. Medina, M. Feijóo, C. Ruiz, E. Moreno, M. M. Gómez, J. A. Collado, C. Muñoz, J. Muntané, P. Montilla, I. Túnez

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In neurodegenerative diseases, progressive oxidative stress is a major event that precedes neuronal death. Oxidative stress is characterized by an imbalance between oxidants and antioxidants. This imbalance induced oxidative molecular and cell damage, reducing cellular viability. 3-Nitropropionic acid (3NP) causes oxidative stress and other molecular and cellular changes similar to those observed in neurons of patients with Huntington's disease. Since carvedilol and melatonin act as free-radical scavengers, this study examined the effect of carvedilol (10-5 M) and melatonin (10-5 M) on oxidative and cell damage induced by 3NP in N1E-115 neuroblastoma cells. Carvedilol and melatonin prevented the increases in lipid peroxidation and total LDH activity, as well as the depletion of reduced glutathione (GSH) and the reduction of antioxidative enzymes activities in NlE-115 cells incubated with 100 mM 3NP. All these carvedilol and melatonin effects were more intense when the drugs were added before rather than after inducing the damage by 3NP. These results also provided evidence supporting the hypothesis that carvedilol and melatonin can be useful for treating neurodegenerative diseases, such as Huntington's disease.

Original languageEnglish (US)
Pages (from-to)291-296
Number of pages6
JournalJournal of Physiology and Biochemistry
Volume65
Issue number3
DOIs
StatePublished - Sep 2009
Externally publishedYes

Fingerprint

Neuroprotective Agents
Melatonin
Neuroblastoma
Oxidative stress
Neurodegenerative diseases
Oxidative Stress
Huntington Disease
Neurodegenerative Diseases
Cells
Free Radical Scavengers
Enzyme activity
Oxidants
Lipid Peroxidation
Neurons
Glutathione
Antioxidants
3-nitropropionic acid
carvedilol
Lipids
Enzymes

Keywords

  • Antioxidative effect
  • Free radical
  • Huntington's disease
  • Neurotoxicity

ASJC Scopus subject areas

  • Physiology
  • Biochemistry

Cite this

Neuroprotective effect of carvedilol and melatonin on 3-nitropropionic acid-induced neurotoxicity in neuroblastoma. / Tasset, Inmaculada; Espínola, C.; Medina, F. J.; Feijóo, M.; Ruiz, C.; Moreno, E.; Gómez, M. M.; Collado, J. A.; Muñoz, C.; Muntané, J.; Montilla, P.; Túnez, I.

In: Journal of Physiology and Biochemistry, Vol. 65, No. 3, 09.2009, p. 291-296.

Research output: Contribution to journalArticle

Tasset, I, Espínola, C, Medina, FJ, Feijóo, M, Ruiz, C, Moreno, E, Gómez, MM, Collado, JA, Muñoz, C, Muntané, J, Montilla, P & Túnez, I 2009, 'Neuroprotective effect of carvedilol and melatonin on 3-nitropropionic acid-induced neurotoxicity in neuroblastoma', Journal of Physiology and Biochemistry, vol. 65, no. 3, pp. 291-296. https://doi.org/10.1007/BF03180581
Tasset, Inmaculada ; Espínola, C. ; Medina, F. J. ; Feijóo, M. ; Ruiz, C. ; Moreno, E. ; Gómez, M. M. ; Collado, J. A. ; Muñoz, C. ; Muntané, J. ; Montilla, P. ; Túnez, I. / Neuroprotective effect of carvedilol and melatonin on 3-nitropropionic acid-induced neurotoxicity in neuroblastoma. In: Journal of Physiology and Biochemistry. 2009 ; Vol. 65, No. 3. pp. 291-296.
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