Nerve injury induces gap junctional coupling among axotomized adult motor neurons

Qiang Chang, Alberto E. Pereda, Martin J. Pinter, Rita J. Balice-Gordon

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Neonatal spinal motor neurons are electrically and dye-coupled by gap junctions, but coupling is transient and disappears rapidly after birth. Here we report that adult motor neurons become recoupled by gap junctions after peripheral nerve injury. One and 4-6 weeks after nerve cut, clusters of dye- coupled motor neurons were observed among axotomized, but not control, lumbar spinal motor neurons in adult cats. Electrical coupling was not apparent, probably because of the electrotonic distance between dendrodendritic gap junctions and the somatic recording location. Analyses of gap junction protein expression in cat and rat showed that the repertoire of connexins expressed by normal adult motor neurons, Cx36, Cx37, Cx40, Cx43, and Cx45, was unchanged after axotomy. Our results suggest that the reestablishment of gap junctional coupling among axotomized adult motor neurons may occur by modulation of existing gap junction proteins that are constitutively expressed by motor neurons. After injury, interneuronal gap junctional coupling may mediate signaling that maintains the viability of axotomized motor neurons until synaptic connections are reestablished within their targets.

Original languageEnglish (US)
Pages (from-to)674-684
Number of pages11
JournalJournal of Neuroscience
Volume20
Issue number2
StatePublished - Jan 15 2000
Externally publishedYes

Fingerprint

Motor Neurons
Wounds and Injuries
Connexins
Gap Junctions
Cats
Coloring Agents
Axotomy
Peripheral Nerve Injuries
Connexin 43
Parturition

Keywords

  • Axotomy
  • Connexin
  • Gap junction
  • Injury
  • Motor neuron
  • Nerve
  • Skeletal muscle

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Nerve injury induces gap junctional coupling among axotomized adult motor neurons. / Chang, Qiang; Pereda, Alberto E.; Pinter, Martin J.; Balice-Gordon, Rita J.

In: Journal of Neuroscience, Vol. 20, No. 2, 15.01.2000, p. 674-684.

Research output: Contribution to journalArticle

Chang, Q, Pereda, AE, Pinter, MJ & Balice-Gordon, RJ 2000, 'Nerve injury induces gap junctional coupling among axotomized adult motor neurons', Journal of Neuroscience, vol. 20, no. 2, pp. 674-684.
Chang, Qiang ; Pereda, Alberto E. ; Pinter, Martin J. ; Balice-Gordon, Rita J. / Nerve injury induces gap junctional coupling among axotomized adult motor neurons. In: Journal of Neuroscience. 2000 ; Vol. 20, No. 2. pp. 674-684.
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