Nerve growth factor production by lymphocytes

Laura Santambrogio, Marta Benedetti, Moses V. Chao, Rana Muzaffar, Kimary Kulig, Nadia Gabellini, Gerald Hochwald

Research output: Contribution to journalArticle

184 Citations (Scopus)

Abstract

Nerve growth factor (NGF) is a neurotrophic protein essential for the maintenance and growth of peripheral sympathetic neurons and basal forebrain cholinergic neurons. Recently, NGF has also been shown to have effects on cells of the immune system. In a search for extra neural sources of NGF, we detected NGF-specific mRNA in mouse T lymphocytes of both the CD4+ and CD8+ phenotypes with the use of an RNase protection assay, PCR, and DNA sequence analysis. In CD4+ cells, NGF was present in both Th1 and Th2 Ag-specific clones, but an increase of NGF-specific message was detected after antigenic stimulation only in Th2 clones. NGF mRNA was also detected in splenic B lymphocytes and in a cell line derived from a murine follicular center cell lymphoma. Translation into protein and secretion of NGF were demonstrated by Western blot analysis. The secreted NGF is in an active form capable of inducing differentiation of PC12 cells into sympathetic-like neurons. Furthermore, conditioned medium from clones or lines positive for NGF mRNA was capable of inducing p140 tyrosine kinase autophosphorylation in 3T3 fibroblasts transfected with cDNA encoding for the tyrosine kinase family NGF receptor. We conclude that lymphocytes synthesize and secrete NGF either as a para-autocrine factor acting on the immune system itself, or as a factor for the maintenance of peripheral neurons.

Original languageEnglish (US)
Pages (from-to)4488-4495
Number of pages8
JournalJournal of Immunology
Volume153
Issue number10
StatePublished - Nov 15 1994
Externally publishedYes

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Nerve Growth Factor
Lymphocytes
Clone Cells
Neurons
Protein-Tyrosine Kinases
Messenger RNA
Immune System
Maintenance
Nerve Growth Factor Receptor
Cholinergic Neurons
PC12 Cells
Nerve Growth Factors
Protein Biosynthesis
Ribonucleases
Conditioned Culture Medium
DNA Sequence Analysis
Lymphoma
B-Lymphocytes
Complementary DNA
Fibroblasts

ASJC Scopus subject areas

  • Immunology

Cite this

Santambrogio, L., Benedetti, M., Chao, M. V., Muzaffar, R., Kulig, K., Gabellini, N., & Hochwald, G. (1994). Nerve growth factor production by lymphocytes. Journal of Immunology, 153(10), 4488-4495.

Nerve growth factor production by lymphocytes. / Santambrogio, Laura; Benedetti, Marta; Chao, Moses V.; Muzaffar, Rana; Kulig, Kimary; Gabellini, Nadia; Hochwald, Gerald.

In: Journal of Immunology, Vol. 153, No. 10, 15.11.1994, p. 4488-4495.

Research output: Contribution to journalArticle

Santambrogio, L, Benedetti, M, Chao, MV, Muzaffar, R, Kulig, K, Gabellini, N & Hochwald, G 1994, 'Nerve growth factor production by lymphocytes', Journal of Immunology, vol. 153, no. 10, pp. 4488-4495.
Santambrogio L, Benedetti M, Chao MV, Muzaffar R, Kulig K, Gabellini N et al. Nerve growth factor production by lymphocytes. Journal of Immunology. 1994 Nov 15;153(10):4488-4495.
Santambrogio, Laura ; Benedetti, Marta ; Chao, Moses V. ; Muzaffar, Rana ; Kulig, Kimary ; Gabellini, Nadia ; Hochwald, Gerald. / Nerve growth factor production by lymphocytes. In: Journal of Immunology. 1994 ; Vol. 153, No. 10. pp. 4488-4495.
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