Neomycin Inhibits Glycoprotein C (gC)-Dependent Binding of Herpes Simplex Virus Type 1 to Cells and Also Inhibits Postbinding Events in Entry

Betsy Herold, Patricia G. Spear

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Previous studies have identified requirements for the binding of herpes simplex virus type 1 (HSV-1) to cells, including the presence of particular glycoproteins in the virion envelope (gC or gB) and the presence of particular glycosaminoglycan chains (principally heparan sulfate) on cell surface proteoglycans. We show here that neomycin, a known inhibitor of HSV infection, blocked early events in HSV infection by two mechanisms: partial inhibition of the gC-dependent binding of virions, but not the gB-dependent binding, and inhibition of events that occurred after the binding of virus to cells. Near-maximal (but incomplete) inhibition of virus binding occurred at low concentrations of neomycin (1 mM) for wild-type and gB-negative virions only. Neomycin also inhibited the binding of Isolated gC to cells at a similar concentration. Concentrations of neomycin as high as 50 mM had little or no effect on the binding of gO-negative virions to cells, Nevertheless, neomycin significantly inhibited infection by both wild-type and gO-negative virions, at concentrations greater than 10 mM, indicating that the inhibition at higher doses was not due to effects on virus binding. The effects of neomycin on virus binding suggest that gC (but not gB) and neomycin compete for binding to similar structural features of cell surface heparan sulfate.

Original languageEnglish (US)
Pages (from-to)166-171
Number of pages6
JournalVirology
Volume203
Issue number1
DOIs
StatePublished - Aug 15 1994
Externally publishedYes

Fingerprint

Neomycin
Human Herpesvirus 1
Virus Attachment
Virion
Heparitin Sulfate
Infection
Defective Viruses
Proteoglycans
glycoprotein gC, herpes simplex virus type 1
Glycosaminoglycans
Glycoproteins

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Neomycin Inhibits Glycoprotein C (gC)-Dependent Binding of Herpes Simplex Virus Type 1 to Cells and Also Inhibits Postbinding Events in Entry. / Herold, Betsy; Spear, Patricia G.

In: Virology, Vol. 203, No. 1, 15.08.1994, p. 166-171.

Research output: Contribution to journalArticle

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