Near-infrared fluorescent proteins for multicolor in vivo imaging

Research output: Contribution to journalArticle

245 Citations (Scopus)

Abstract

Near-infrared fluorescent proteins (FPs) are in high demand for in vivo imaging. We developed four spectrally distinct near-infrared FPs - iRFP670, iRFP682, iRFP702 and iRFP720 - from bacterial phytochromes. iRFPs exhibit high brightness in mammalian cells and tissues and are suitable for long-term studies. iRFP670 and iRFP720 enable two-color imaging with standard approaches in living cells and mice. The four new iRFPs and the previously engineered iRFP713 allow multicolor imaging with spectral unmixing in living mice.

Original languageEnglish (US)
Pages (from-to)751-754
Number of pages4
JournalNature Methods
Volume10
Issue number8
DOIs
StatePublished - Aug 2013

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Phytochrome
Infrared radiation
Imaging techniques
Cells
Proteins
Color
Luminance
Tissue

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Near-infrared fluorescent proteins for multicolor in vivo imaging. / Shcherbakova, Daria; Verkhusha, Vladislav.

In: Nature Methods, Vol. 10, No. 8, 08.2013, p. 751-754.

Research output: Contribution to journalArticle

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