Near-infrared fluorescent proteins for multicolor in vivo imaging

Research output: Contribution to journalArticlepeer-review

393 Scopus citations


Near-infrared fluorescent proteins (FPs) are in high demand for in vivo imaging. We developed four spectrally distinct near-infrared FPs - iRFP670, iRFP682, iRFP702 and iRFP720 - from bacterial phytochromes. iRFPs exhibit high brightness in mammalian cells and tissues and are suitable for long-term studies. iRFP670 and iRFP720 enable two-color imaging with standard approaches in living cells and mice. The four new iRFPs and the previously engineered iRFP713 allow multicolor imaging with spectral unmixing in living mice.

Original languageEnglish (US)
Pages (from-to)751-754
Number of pages4
JournalNature Methods
Issue number8
StatePublished - Aug 2013

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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