Nanoscale imaging of molecular positions and anisotropies

Travis J. Gould; Mudalige S. Gunewardene; Manasa V. Gudheti; Vladislav V. Verkhusha; Shu Rong Yin; Julie A. Gosse; Samuel T. Hess

doi:10.1038/nmeth.1271

Nanoscale imaging of molecular positions and anisotropies

Travis J. Gould, Mudalige S. Gunewardene, Manasa V. Gudheti, Vladislav V. Verkhusha, Shu Rong Yin, Julie A. Gosse, Samuel T. Hess

Anatomy & Structural Biology

Research output: Contribution to journal › Article

87 Scopus citations

Abstract

Knowledge of the orientation of molecules within biological structures is crucial to understanding the mechanisms of cell function. We present a method to image simultaneously the positions and fluorescence anisotropies of large numbers of single molecules with nanometer lateral resolution within a sample. Based on a simple modification of fluorescence photoactivation localization microscopy (FPALM), polarization (P)-FPALM does not compromise speed or sensitivity. We show results for mouse fibroblasts expressing Dendra2-actin or Dendra2-hemagglutinin.

Original language	English (US)
Pages (from-to)	1027-1030
Number of pages	4
Journal	Nature Methods
Volume	5
Issue number	12
DOIs	https://doi.org/10.1038/nmeth.1271
Publication status	Published - Nov 14 2008

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ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

Cite this

Gould, T. J., Gunewardene, M. S., Gudheti, M. V., Verkhusha, V. V., Yin, S. R., Gosse, J. A., & Hess, S. T. (2008). Nanoscale imaging of molecular positions and anisotropies. Nature Methods, 5(12), 1027-1030. https://doi.org/10.1038/nmeth.1271

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AU - Gosse, Julie A.

AU - Hess, Samuel T.

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10.1038/nmeth.1271