Nanoscale imaging of molecular positions and anisotropies

Travis J. Gould; Mudalige S. Gunewardene; Manasa V. Gudheti; Vladislav V. Verkhusha; Shu Rong Yin; Julie A. Gosse; Samuel T. Hess

doi:10.1038/nmeth.1271

Nanoscale imaging of molecular positions and anisotropies

Travis J. Gould, Mudalige S. Gunewardene, Manasa V. Gudheti, Vladislav V. Verkhusha, Shu Rong Yin, Julie A. Gosse, Samuel T. Hess

Anatomy & Structural Biology

Research output: Contribution to journal › Article

87 Scopus citations

Abstract

Knowledge of the orientation of molecules within biological structures is crucial to understanding the mechanisms of cell function. We present a method to image simultaneously the positions and fluorescence anisotropies of large numbers of single molecules with nanometer lateral resolution within a sample. Based on a simple modification of fluorescence photoactivation localization microscopy (FPALM), polarization (P)-FPALM does not compromise speed or sensitivity. We show results for mouse fibroblasts expressing Dendra2-actin or Dendra2-hemagglutinin.

Original language	English (US)
Pages (from-to)	1027-1030
Number of pages	4
Journal	Nature Methods
Volume	5
Issue number	12
DOIs	https://doi.org/10.1038/nmeth.1271
State	Published - Nov 14 2008

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1038/nmeth.1271

Fingerprint Dive into the research topics of 'Nanoscale imaging of molecular positions and anisotropies'. Together they form a unique fingerprint.

Cite this

Gould, T. J., Gunewardene, M. S., Gudheti, M. V., Verkhusha, V. V., Yin, S. R., Gosse, J. A., & Hess, S. T. (2008). Nanoscale imaging of molecular positions and anisotropies. Nature Methods, 5(12), 1027-1030. https://doi.org/10.1038/nmeth.1271

@article{5b4bc0acb548477799e7160ceab47710,

title = "Nanoscale imaging of molecular positions and anisotropies",

abstract = "Knowledge of the orientation of molecules within biological structures is crucial to understanding the mechanisms of cell function. We present a method to image simultaneously the positions and fluorescence anisotropies of large numbers of single molecules with nanometer lateral resolution within a sample. Based on a simple modification of fluorescence photoactivation localization microscopy (FPALM), polarization (P)-FPALM does not compromise speed or sensitivity. We show results for mouse fibroblasts expressing Dendra2-actin or Dendra2-hemagglutinin.",

author = "Gould, {Travis J.} and Gunewardene, {Mudalige S.} and Gudheti, {Manasa V.} and Verkhusha, {Vladislav V.} and Yin, {Shu Rong} and Gosse, {Julie A.} and Hess, {Samuel T.}",

year = "2008",

month = nov,

day = "14",

doi = "10.1038/nmeth.1271",

language = "English (US)",

volume = "5",

pages = "1027--1030",

journal = "Nature Methods",

issn = "1548-7091",

publisher = "Nature Publishing Group",

number = "12",

}

TY - JOUR

T1 - Nanoscale imaging of molecular positions and anisotropies

AU - Gould, Travis J.

AU - Gunewardene, Mudalige S.

AU - Gudheti, Manasa V.

AU - Verkhusha, Vladislav V.

AU - Yin, Shu Rong

AU - Gosse, Julie A.

AU - Hess, Samuel T.

PY - 2008/11/14

Y1 - 2008/11/14

N2 - Knowledge of the orientation of molecules within biological structures is crucial to understanding the mechanisms of cell function. We present a method to image simultaneously the positions and fluorescence anisotropies of large numbers of single molecules with nanometer lateral resolution within a sample. Based on a simple modification of fluorescence photoactivation localization microscopy (FPALM), polarization (P)-FPALM does not compromise speed or sensitivity. We show results for mouse fibroblasts expressing Dendra2-actin or Dendra2-hemagglutinin.

AB - Knowledge of the orientation of molecules within biological structures is crucial to understanding the mechanisms of cell function. We present a method to image simultaneously the positions and fluorescence anisotropies of large numbers of single molecules with nanometer lateral resolution within a sample. Based on a simple modification of fluorescence photoactivation localization microscopy (FPALM), polarization (P)-FPALM does not compromise speed or sensitivity. We show results for mouse fibroblasts expressing Dendra2-actin or Dendra2-hemagglutinin.

UR - http://www.scopus.com/inward/record.url?scp=57049135688&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57049135688&partnerID=8YFLogxK

U2 - 10.1038/nmeth.1271

DO - 10.1038/nmeth.1271

M3 - Article

C2 - 19011626

AN - SCOPUS:57049135688

VL - 5

SP - 1027

EP - 1030

JO - Nature Methods

JF - Nature Methods

SN - 1548-7091

IS - 12

ER -