Nanoscale imaging of molecular positions and anisotropies

Travis J. Gould; Mudalige S. Gunewardene; Manasa V. Gudheti; Vladislav V. Verkhusha; Shu Rong Yin; Julie A. Gosse; Samuel T. Hess

doi:10.1038/nmeth.1271

Nanoscale imaging of molecular positions and anisotropies

Travis J. Gould, Mudalige S. Gunewardene, Manasa V. Gudheti, Vladislav V. Verkhusha, Shu Rong Yin, Julie A. Gosse, Samuel T. Hess

Anatomy & Structural Biology

Research output: Contribution to journal › Article › peer-review

87 Scopus citations

Abstract

Knowledge of the orientation of molecules within biological structures is crucial to understanding the mechanisms of cell function. We present a method to image simultaneously the positions and fluorescence anisotropies of large numbers of single molecules with nanometer lateral resolution within a sample. Based on a simple modification of fluorescence photoactivation localization microscopy (FPALM), polarization (P)-FPALM does not compromise speed or sensitivity. We show results for mouse fibroblasts expressing Dendra2-actin or Dendra2-hemagglutinin.

Original language	English (US)
Pages (from-to)	1027-1030
Number of pages	4
Journal	Nature Methods
Volume	5
Issue number	12
DOIs	https://doi.org/10.1038/nmeth.1271
State	Published - Nov 14 2008

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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AU - Gunewardene, Mudalige S.

AU - Gudheti, Manasa V.

AU - Verkhusha, Vladislav V.

AU - Yin, Shu Rong

AU - Gosse, Julie A.

AU - Hess, Samuel T.

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