@article{5b4bc0acb548477799e7160ceab47710,
title = "Nanoscale imaging of molecular positions and anisotropies",
abstract = "Knowledge of the orientation of molecules within biological structures is crucial to understanding the mechanisms of cell function. We present a method to image simultaneously the positions and fluorescence anisotropies of large numbers of single molecules with nanometer lateral resolution within a sample. Based on a simple modification of fluorescence photoactivation localization microscopy (FPALM), polarization (P)-FPALM does not compromise speed or sensitivity. We show results for mouse fibroblasts expressing Dendra2-actin or Dendra2-hemagglutinin.",
author = "Gould, {Travis J.} and Gunewardene, {Mudalige S.} and Gudheti, {Manasa V.} and Verkhusha, {Vladislav V.} and Yin, {Shu Rong} and Gosse, {Julie A.} and Hess, {Samuel T.}",
note = "Funding Information: We thank C. Fang-Yen, P. Blank, J. Bewersdorf, J. Zimmerberg and M. Mason for useful discussions, G. Patterson (US National Institute of Child Health and Human Development) for providing the construct encoding the PA-GFP protein, J. Shim, J. Rochira and E. Allgeyer for laboratory assistance, A. McGinn, T. Tripp and P. Byard for professional services. This work was supported by grants K25-65459 from the US National Institute of Allergy and Infectious Diseases, CHE-0722759 from the National Science Foundation, start up funds from the University of Maine (S.T.H.), and by grants GM070358 and GM073913 from the National Institute of General Medical Sciences (V.V.V.).",
year = "2008",
doi = "10.1038/nmeth.1271",
language = "English (US)",
volume = "5",
pages = "1027--1030",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "12",
}