Nanoscale imaging of molecular positions and anisotropies

Travis J. Gould, Mudalige S. Gunewardene, Manasa V. Gudheti, Vladislav V. Verkhusha, Shu Rong Yin, Julie A. Gosse, Samuel T. Hess

Research output: Contribution to journalArticle

87 Scopus citations

Abstract

Knowledge of the orientation of molecules within biological structures is crucial to understanding the mechanisms of cell function. We present a method to image simultaneously the positions and fluorescence anisotropies of large numbers of single molecules with nanometer lateral resolution within a sample. Based on a simple modification of fluorescence photoactivation localization microscopy (FPALM), polarization (P)-FPALM does not compromise speed or sensitivity. We show results for mouse fibroblasts expressing Dendra2-actin or Dendra2-hemagglutinin.

Original languageEnglish (US)
Pages (from-to)1027-1030
Number of pages4
JournalNature Methods
Volume5
Issue number12
DOIs
Publication statusPublished - Nov 14 2008

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ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Gould, T. J., Gunewardene, M. S., Gudheti, M. V., Verkhusha, V. V., Yin, S. R., Gosse, J. A., & Hess, S. T. (2008). Nanoscale imaging of molecular positions and anisotropies. Nature Methods, 5(12), 1027-1030. https://doi.org/10.1038/nmeth.1271