Nanopatterned muscle cell patches for enhanced myogenesis and dystrophin expression in a mouse model of muscular dystrophy

Hee Seok Yang, Nicholas Ieronimakis, Jonathan H. Tsui, Hong Nam Kim, Kahp Yang Suh, Morayma Reyes Gil, Deok Ho Kim

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Skeletal muscle is a highly organized tissue in which the extracellular matrix (ECM) is composed of highly-aligned cables of collagen with nanoscale feature sizes, and provides structural and functional support to muscle fibers. As such, the transplantation of disorganized tissues or the direct injection of cells into muscles for regenerative therapy often results in suboptimal functional improvement due to a failure to integrate with native tissue properly. Here, we present a simple method in which biodegradable, biomimetic substrates with precisely controlled nanotopography were fabricated using solvent-assisted capillary force lithography (CFL) and were able to induce the proper development and differentiation of primary mononucleated cells to form mature muscle patches. Cells cultured on these nanopatterned substrates were highly-aligned and elongated, and formed more mature myotubes as evidenced by up-regulated expression of the myogenic regulatory factors Myf5, MyoD and myogenin (MyoG). When transplanted into mdx mice models for Duchenne muscular dystrophy (DMD), the proposed muscle patches led to the formation of a significantly greater number of dystrophin-positive muscle fibers, indicating that dystrophin replacement and myogenesis is achievable invivo with this approach. These results demonstrate the feasibility of utilizing biomimetic substrates not only as platforms for studying the influences of the ECM on skeletal muscle function and maturation, but also to create transplantable muscle cell patches for the treatment of chronic and acute muscle diseases or injuries.

Original languageEnglish (US)
Pages (from-to)1478-1486
Number of pages9
JournalBiomaterials
Volume35
Issue number5
DOIs
StatePublished - Feb 2014
Externally publishedYes

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Dystrophin
Muscle Development
Muscular Dystrophies
Muscle Cells
Muscle
Cells
Muscles
Biomimetics
Extracellular Matrix
Skeletal Muscle
Myogenic Regulatory Factors
Inbred mdx Mouse
Myogenin
Tissue Transplantation
Duchenne Muscular Dystrophy
Tissue
Skeletal Muscle Fibers
Acute Disease
Substrates
Cultured Cells

Keywords

  • Muscle tissue engineering
  • Muscular dystrophy
  • Myogenesis
  • Nanotopography
  • Poly(lactic-co-glycolic acid)

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Nanopatterned muscle cell patches for enhanced myogenesis and dystrophin expression in a mouse model of muscular dystrophy. / Yang, Hee Seok; Ieronimakis, Nicholas; Tsui, Jonathan H.; Kim, Hong Nam; Suh, Kahp Yang; Reyes Gil, Morayma; Kim, Deok Ho.

In: Biomaterials, Vol. 35, No. 5, 02.2014, p. 1478-1486.

Research output: Contribution to journalArticle

Yang, Hee Seok ; Ieronimakis, Nicholas ; Tsui, Jonathan H. ; Kim, Hong Nam ; Suh, Kahp Yang ; Reyes Gil, Morayma ; Kim, Deok Ho. / Nanopatterned muscle cell patches for enhanced myogenesis and dystrophin expression in a mouse model of muscular dystrophy. In: Biomaterials. 2014 ; Vol. 35, No. 5. pp. 1478-1486.
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