N-Acetylglucosamine modulates function of the skin fibroblasts

A. Połubinska, J. Cwalinski, E. Baum, A. Brȩborowicz

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Synopsis Background Fibroblasts are an important component of the skin determining its properties. N-Acetylglucosamine (NAG) is the substrate for hyaluronan synthesis, and it also has anti-inflammatory and anti-senescent activity in mesothelial cells. Methods We tested in in vitro-cultured human skin fibroblasts how supplementation of culture medium with NAG 10 mmol L -1 changes properties of these cells. Results Fibroblasts cultured in presence of NAG produced more proteins and that was mainly due to increased synthesis of collagen (+33% vs. control, P < 0.05). Hyaluronan synthesis was increased (+107% vs. control, P < 0.001), but interleukin-6 synthesis was reduced (-22% vs. control, P < 0.05). Fibroblasts cultured in medium with NAG 10 mmol L-1 demonstrated improved ability to heal the injured layer of cells (+34% vs. control, P < 0.05). Additionally senescence of fibroblasts undergoing replicative ageing in the presence of NAG was less pronounced, as reflected by smaller increase in the population doubling time (-70% vs. control, P < 0.05). Conclusion We conclude that NAG induced changes in the skin fibroblasts' properties maybe important for prevention of the age-dependent changes in its structure and function.

Original languageEnglish (US)
Pages (from-to)472-476
Number of pages5
JournalInternational Journal of Cosmetic Science
Volume35
Issue number5
DOIs
StatePublished - Oct 1 2013

Keywords

  • N-Acetylglucosamine
  • collagen
  • dermal fibroblasts
  • hyaluronan
  • inflammation
  • senescence

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Aging
  • Pharmaceutical Science
  • Drug Discovery
  • Dermatology
  • Colloid and Surface Chemistry

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