Abstract
Synopsis Background Fibroblasts are an important component of the skin determining its properties. N-Acetylglucosamine (NAG) is the substrate for hyaluronan synthesis, and it also has anti-inflammatory and anti-senescent activity in mesothelial cells. Methods We tested in in vitro-cultured human skin fibroblasts how supplementation of culture medium with NAG 10 mmol L -1 changes properties of these cells. Results Fibroblasts cultured in presence of NAG produced more proteins and that was mainly due to increased synthesis of collagen (+33% vs. control, P < 0.05). Hyaluronan synthesis was increased (+107% vs. control, P < 0.001), but interleukin-6 synthesis was reduced (-22% vs. control, P < 0.05). Fibroblasts cultured in medium with NAG 10 mmol L-1 demonstrated improved ability to heal the injured layer of cells (+34% vs. control, P < 0.05). Additionally senescence of fibroblasts undergoing replicative ageing in the presence of NAG was less pronounced, as reflected by smaller increase in the population doubling time (-70% vs. control, P < 0.05). Conclusion We conclude that NAG induced changes in the skin fibroblasts' properties maybe important for prevention of the age-dependent changes in its structure and function.
Original language | English (US) |
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Pages (from-to) | 472-476 |
Number of pages | 5 |
Journal | International Journal of Cosmetic Science |
Volume | 35 |
Issue number | 5 |
DOIs | |
State | Published - Oct 2013 |
Externally published | Yes |
Keywords
- N-Acetylglucosamine
- collagen
- dermal fibroblasts
- hyaluronan
- inflammation
- senescence
ASJC Scopus subject areas
- Chemistry (miscellaneous)
- Aging
- Pharmaceutical Science
- Drug Discovery
- Dermatology
- Colloid and Surface Chemistry