N-acetylcysteine S-nitrosothiol nanoparticles prevent wound expansion and accelerate wound closure in a murine burn model

Angelo Landriscina, Tagai Musaev, Jamie Rosen, Anjana Ray, Parimala Nacharaju, Joshua D. Nosanchuk, Adam J. Friedman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: The treatment of cutaneous wounds in the clinical setting continues to be a clinical challenge and economic burden, with burn wounds being especially formidable. Direct mechanical injury coupled with the transfer of thermal energy leads to tissue necrosis, pro-inflammatory cytokine release and the eventual expansion of an initial wound. Our current therapeutic armamentarium falls short of options to help prevent wound expansion, and therefore new modalities are required. Nitrosating substances such as RSNOs have been proven to be effective in promoting wound closure due to their ability to modulate inflammation, cytokine production and vascular function. Objective: We aim to evaluate the efficacy of n-actetylcysteine s-nitrosothiol nanoparticles (NAC-SNO-np) on thermal burn wounds and associated expansion. Methods: A multi-bum model was utilized to induce three burn wounds on the dorsal surface of BALB/c mice, allowing for evaluation of the burn itself and peripheral tissue. Wounds were excised and processed for histology and immunohistochemistry on day 7 following wounding. Results: Following treatment with NAC-SNO-np, burn wound expansion was attenuated and wound healing was accelerated. Histological analysis revealed increased collagen deposition as well as increased macrophage and decreased neutrophil infiltration into the wound bed. Conclusion: NAC-SNO-np represents a platform that harnesses the nitrosative properties of NAC-SNO in order to accelerate the transition from inflammatory to proliferative wound healing. Further studies are needed in order to translate to the clinical setting.

Original languageEnglish (US)
Pages (from-to)726-732
Number of pages7
JournalJournal of Drugs in Dermatology
Volume14
Issue number7
StatePublished - Jul 1 2015

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S-Nitrosothiols
Acetylcysteine
Nanoparticles
Wounds and Injuries
Wound Healing
Hot Temperature
Cytokines
Neutrophil Infiltration
Energy Transfer
Blood Vessels
Histology

ASJC Scopus subject areas

  • Dermatology

Cite this

N-acetylcysteine S-nitrosothiol nanoparticles prevent wound expansion and accelerate wound closure in a murine burn model. / Landriscina, Angelo; Musaev, Tagai; Rosen, Jamie; Ray, Anjana; Nacharaju, Parimala; Nosanchuk, Joshua D.; Friedman, Adam J.

In: Journal of Drugs in Dermatology, Vol. 14, No. 7, 01.07.2015, p. 726-732.

Research output: Contribution to journalArticle

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AU - Nacharaju, Parimala

AU - Nosanchuk, Joshua D.

AU - Friedman, Adam J.

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