Myocardial expression of endothelin-1 in murine Trypanosoma cruzi infection

Stefka B. Petkova, Herbert B. Tanowitz, Harold I. Magazine, Stephen M. Factor, John Chan, Richard G. Pestell, Boumediene Bouzahzah, Stephen A. Douglas, Vitaliy Shtutin, Stephen A. Morris, Enders Tsang, Louis M. Weiss, George J. Christ, Murray Wittner, Huan Huang

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Chagas' disease, caused by Trypanosoma cruzi, is an important cause of myocarditis and chronic cardiomyopathy and is accompanied by microvascular spasm and myocardial ischemia. We reported previously that infection of cultured endothelial cells with T. cruzi increased the synthesis of biologically active endothlein-1 (ET-1). In the present study, we examined the role of ET-1 in the cardiovascular system of CD1 mice infected with the Brazil strain of T. cruzi and C57BL/6 mice infected with the Tulahuen strain during acute infection. In the myocardium of infected mice myonecrosis and multiple pseudocysts were observed. There was also an intense vasculitis of the aorta, coronary artery, smaller myocardial vessels and the endocardial endothelium. Immunohistochemistry studies employing anti-ET-1 antibody revealed increased expression of ET-1 that was most intense in the endocardial and vascular endothelium. Elevated levels of mRNA for preproET-1, endothelin converting enzyme and ET-1 were observed in the same myocardial samples. Plasma ET-1 levels were significantly elevated in infected CD1 mice 10-15 days post infection. These observations suggest that increased levels of ET-1 are a consequence of the initial invasion of the cardiovascular system and provide a mechanism for infection-associated myocardial dysfunction. (C) 2000 Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)257-265
Number of pages9
JournalCardiovascular Pathology
Volume9
Issue number5
DOIs
StatePublished - 2000

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Trypanosoma cruzi
Endothelin-1
Cardiovascular System
Infection
Chagas Disease
Myocarditis
Vascular Endothelium
Spasm
Vasculitis
Cardiomyopathies
Inbred C57BL Mouse
Endothelium
Myocardial Ischemia
Brazil
Aorta
Cultured Cells
Coronary Vessels
Myocardium
Endothelial Cells
Immunohistochemistry

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pathology and Forensic Medicine

Cite this

Myocardial expression of endothelin-1 in murine Trypanosoma cruzi infection. / Petkova, Stefka B.; Tanowitz, Herbert B.; Magazine, Harold I.; Factor, Stephen M.; Chan, John; Pestell, Richard G.; Bouzahzah, Boumediene; Douglas, Stephen A.; Shtutin, Vitaliy; Morris, Stephen A.; Tsang, Enders; Weiss, Louis M.; Christ, George J.; Wittner, Murray; Huang, Huan.

In: Cardiovascular Pathology, Vol. 9, No. 5, 2000, p. 257-265.

Research output: Contribution to journalArticle

Petkova, SB, Tanowitz, HB, Magazine, HI, Factor, SM, Chan, J, Pestell, RG, Bouzahzah, B, Douglas, SA, Shtutin, V, Morris, SA, Tsang, E, Weiss, LM, Christ, GJ, Wittner, M & Huang, H 2000, 'Myocardial expression of endothelin-1 in murine Trypanosoma cruzi infection', Cardiovascular Pathology, vol. 9, no. 5, pp. 257-265. https://doi.org/10.1016/S1054-8807(00)00045-4
Petkova, Stefka B. ; Tanowitz, Herbert B. ; Magazine, Harold I. ; Factor, Stephen M. ; Chan, John ; Pestell, Richard G. ; Bouzahzah, Boumediene ; Douglas, Stephen A. ; Shtutin, Vitaliy ; Morris, Stephen A. ; Tsang, Enders ; Weiss, Louis M. ; Christ, George J. ; Wittner, Murray ; Huang, Huan. / Myocardial expression of endothelin-1 in murine Trypanosoma cruzi infection. In: Cardiovascular Pathology. 2000 ; Vol. 9, No. 5. pp. 257-265.
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