Myc-Dependent Genome Instability and Lifespan in Drosophila

Christina Greer, Moonsook Lee, Maaike Westerhof, Brandon Milholland, Rebecca Spokony, Jan Vijg, Julie Secombe

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The Myc family of transcription factors are key regulators of cell growth and proliferation that are dysregulated in a large number of human cancers. When overexpressed, Myc family proteins also cause genomic instability, a hallmark of both transformed and aging cells. Using an in vivo lacZ mutation reporter, we show that overexpression of Myc in Drosophila increases the frequency of large genome rearrangements associated with erroneous repair of DNA double-strand breaks (DSBs). In addition, we find that overexpression of Myc shortens adult lifespan and, conversely, that Myc haploinsufficiency reduces mutation load and extends lifespan. Our data provide the first evidence that Myc may act as a pro-aging factor, possibly through its ability to greatly increase genome instability.

Original languageEnglish (US)
Article numbere74641
JournalPLoS One
Volume8
Issue number9
DOIs
StatePublished - Sep 6 2013

Fingerprint

Genomic Instability
Drosophila
Genes
Aging of materials
mutation
Haploinsufficiency
Mutation
genome
Double-Stranded DNA Breaks
Cell Aging
Cell proliferation
Cell growth
DNA repair
cell growth
cell proliferation
Repair
Transcription Factors
transcription factors
Cell Proliferation
Genome

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Greer, C., Lee, M., Westerhof, M., Milholland, B., Spokony, R., Vijg, J., & Secombe, J. (2013). Myc-Dependent Genome Instability and Lifespan in Drosophila. PLoS One, 8(9), [e74641]. https://doi.org/10.1371/journal.pone.0074641

Myc-Dependent Genome Instability and Lifespan in Drosophila. / Greer, Christina; Lee, Moonsook; Westerhof, Maaike; Milholland, Brandon; Spokony, Rebecca; Vijg, Jan; Secombe, Julie.

In: PLoS One, Vol. 8, No. 9, e74641, 06.09.2013.

Research output: Contribution to journalArticle

Greer, C, Lee, M, Westerhof, M, Milholland, B, Spokony, R, Vijg, J & Secombe, J 2013, 'Myc-Dependent Genome Instability and Lifespan in Drosophila', PLoS One, vol. 8, no. 9, e74641. https://doi.org/10.1371/journal.pone.0074641
Greer C, Lee M, Westerhof M, Milholland B, Spokony R, Vijg J et al. Myc-Dependent Genome Instability and Lifespan in Drosophila. PLoS One. 2013 Sep 6;8(9). e74641. https://doi.org/10.1371/journal.pone.0074641
Greer, Christina ; Lee, Moonsook ; Westerhof, Maaike ; Milholland, Brandon ; Spokony, Rebecca ; Vijg, Jan ; Secombe, Julie. / Myc-Dependent Genome Instability and Lifespan in Drosophila. In: PLoS One. 2013 ; Vol. 8, No. 9.
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