MYBL2, a link between proliferation and differentiation in maturing colon epithelial cells

Michael Papetti, Leonard H. Augenlicht

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Multiple signals, controlling both proliferation and differentiation, must be integrated in the reprogramming of intestinal epithelial cells during maturation along the crypt-luminal axis. The v-myb family member Mybl2, a molecule implicated in the development and maintenance of the stem cell phenotype, has been suggested to play an important role in proliferation and differentiation of several cell types and is a gene we have found is commonly regulated in several systems of colon cell maturation both in vitro and in vivo. Here we show that siRNA silencing of Mybl2 in proliferating Caco-2 cells increases expression of the cell-cycle regulators cdk2, cyclin D2, and c-myc and decreases expression of cdc25B and cyclin B2 with a consequent 10% increase of cells in G2/M and a complementary 10% decrease in G1. Mybl2 occupies sequences upstream of transcriptional start sites of cyclin D2, c-myc, cyclin B2, and cdc25B and regulates reporter activity driven by upstream regions of cdk2, cyclin D2, and c-myc. These data suggest that Mybl2 plays a subtle but key role in linking specific aspects of cell-cycle progression with generation of signals for differentiation and may therefore be fundamental in commitment of intestinal epithelial cells to differentiation pathways during their maturation.

Original languageEnglish (US)
Pages (from-to)785-791
Number of pages7
JournalJournal of Cellular Physiology
Volume226
Issue number3
DOIs
StatePublished - Mar 2011

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Cyclin D2
Cyclin B2
Colon
Epithelial Cells
Cells
Cell Differentiation
Cell Cycle
Caco-2 Cells
Stem cells
Small Interfering RNA
Stem Cells
Genes
Maintenance
Phenotype
Molecules

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

MYBL2, a link between proliferation and differentiation in maturing colon epithelial cells. / Papetti, Michael; Augenlicht, Leonard H.

In: Journal of Cellular Physiology, Vol. 226, No. 3, 03.2011, p. 785-791.

Research output: Contribution to journalArticle

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