Mutations in vacuolar H+-ATPase subunits lead to biliary developmental defects in zebrafish

Steven F. EauClaire, Shuang Cui, Liyuan Ma, James Matous, Florence L. Marlow, Tripti Gupta, Harold A. Burgess, Elliott W. Abrams, Lee D. Kapp, Michael Granato, Mary C. Mullins, Randolph P. Matthews

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

We identified three zebrafish mutants with defects in biliary development. One of these mutants, pekin (pn), also demonstrated generalized hypopigmentation and other defects, including disruption of retinal cell layers, lack of zymogen granules in the pancreas, and dilated Golgi in intestinal epithelial cells. Bile duct cells in pn demonstrated an accumulation of electron dense bodies. We determined that the causative defect in pn was a splice site mutation in the atp6ap2 gene that leads to an inframe stop codon. atp6ap2 encodes a subunit of the vacuolar H+-ATPase (V-H+-ATPase), which modulates pH in intracellular compartments. The Atp6ap2 subunit has also been shown to function as an intracellular renin receptor that stimulates fibrogenesis. Here we show that mutants and morphants involving other V-H+-ATPase subunits also demonstrated developmental biliary defects, but did not demonstrate the inhibition of fibrogenic genes observed in pn. The defects in pn are reminiscent of those we and others have observed in class C VPS (vacuolar protein sorting) family mutants and morphants, and we report here that knockdown of atp6ap2 and vps33b had an additive negative effect on biliary development. Our findings suggest that pathways which are important in modulating intracompartmental pH lead to defects in digestive organ development, and support previous studies demonstrating the importance of intracellular sorting pathways in biliary development.

Original languageEnglish (US)
Pages (from-to)434-444
Number of pages11
JournalDevelopmental Biology
Volume365
Issue number2
DOIs
StatePublished - May 15 2012

Keywords

  • Hepatobiliary
  • Mutagenesis screen
  • Trafficking
  • Zebrafish

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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    EauClaire, S. F., Cui, S., Ma, L., Matous, J., Marlow, F. L., Gupta, T., Burgess, H. A., Abrams, E. W., Kapp, L. D., Granato, M., Mullins, M. C., & Matthews, R. P. (2012). Mutations in vacuolar H+-ATPase subunits lead to biliary developmental defects in zebrafish. Developmental Biology, 365(2), 434-444. https://doi.org/10.1016/j.ydbio.2012.03.009