Murine CD1d-restricted T cell recognition of cellular lipids

Jenny E. Gumperz, Roy Christopher, Anna Makowska, Deirdre Lum, Masahiko Sugita, Theresa Podrebarac, Yasuhiko Koezuka, Steven A. Porcelli, Susanna Cardell, Michael B. Brenner, Samuel M. Behar

Research output: Contribution to journalArticlepeer-review

405 Scopus citations

Abstract

NKT cells are associated with immunological control of autoimmune disease and cancer and can recognize cell surface mCD1d without addition of exogenous antigens. Cellular antigens presented by mCD1d have not been identified, although NKT cells can recognize a synthetic glycolipid, α- GalCer. Here we show that after addition of a lipid extract from a tumor cell line, plate-bound mCD1d molecules stimulated an NKT cell hybridoma. This hybridoma also responded strongly to three purified phospholipids, but failed to recognize α-GalCer. Seven of sixteen other mCD1d restricted hybridomas also showed a response to certain purified phospholipids. These findings suggest NKT cells can recognize cellular antigens distinct from α-GalCer and identify phospholipids as potential self-antigens presented by mCD1d.

Original languageEnglish (US)
Pages (from-to)211-221
Number of pages11
JournalImmunity
Volume12
Issue number2
DOIs
StatePublished - Feb 2000
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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