Murine CD1d-restricted T cell recognition of cellular lipids

Jenny E. Gumperz; Roy Christopher; Anna Makowska; Deirdre Lum; Masahiko Sugita; Theresa Podrebarac; Yasuhiko Koezuka; Steven A. Porcelli; Susanna Cardell; Michael B. Brenner; Samuel M. Behar

doi:10.1016/S1074-7613(00)80174-0

Murine CD1d-restricted T cell recognition of cellular lipids

Jenny E. Gumperz, Roy Christopher, Anna Makowska, Deirdre Lum, Masahiko Sugita, Theresa Podrebarac, Yasuhiko Koezuka, Steven A. Porcelli, Susanna Cardell, Michael B. Brenner, Samuel M. Behar

Research output: Contribution to journal › Article › peer-review

422 Scopus citations

Abstract

NKT cells are associated with immunological control of autoimmune disease and cancer and can recognize cell surface mCD1d without addition of exogenous antigens. Cellular antigens presented by mCD1d have not been identified, although NKT cells can recognize a synthetic glycolipid, α- GalCer. Here we show that after addition of a lipid extract from a tumor cell line, plate-bound mCD1d molecules stimulated an NKT cell hybridoma. This hybridoma also responded strongly to three purified phospholipids, but failed to recognize α-GalCer. Seven of sixteen other mCD1d restricted hybridomas also showed a response to certain purified phospholipids. These findings suggest NKT cells can recognize cellular antigens distinct from α-GalCer and identify phospholipids as potential self-antigens presented by mCD1d.

Original language	English (US)
Pages (from-to)	211-221
Number of pages	11
Journal	Immunity
Volume	12
Issue number	2
DOIs	https://doi.org/10.1016/S1074-7613(00)80174-0
State	Published - Feb 2000
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S1074-7613(00)80174-0

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title = "Murine CD1d-restricted T cell recognition of cellular lipids",

abstract = "NKT cells are associated with immunological control of autoimmune disease and cancer and can recognize cell surface mCD1d without addition of exogenous antigens. Cellular antigens presented by mCD1d have not been identified, although NKT cells can recognize a synthetic glycolipid, α- GalCer. Here we show that after addition of a lipid extract from a tumor cell line, plate-bound mCD1d molecules stimulated an NKT cell hybridoma. This hybridoma also responded strongly to three purified phospholipids, but failed to recognize α-GalCer. Seven of sixteen other mCD1d restricted hybridomas also showed a response to certain purified phospholipids. These findings suggest NKT cells can recognize cellular antigens distinct from α-GalCer and identify phospholipids as potential self-antigens presented by mCD1d.",

author = "Gumperz, {Jenny E.} and Roy Christopher and Anna Makowska and Deirdre Lum and Masahiko Sugita and Theresa Podrebarac and Yasuhiko Koezuka and Porcelli, {Steven A.} and Susanna Cardell and Brenner, {Michael B.} and Behar, {Samuel M.}",

note = "Funding Information: J. E. G. is a Burroughs Wellcome Fellow of the Life Sciences Research Foundation. M. S. was suported by the Leukemia Society of America, T. P. by the Medical Research Council of Canada, and S. P. by the American Cancer Society and Arthritis Foundation. This work was supported by National Institutes of Health grants AR01978 to S. M. B. and AI28973 to M. B. B.",

year = "2000",

month = feb,

doi = "10.1016/S1074-7613(00)80174-0",

language = "English (US)",

volume = "12",

pages = "211--221",

journal = "Immunity",

issn = "1074-7613",

publisher = "Cell Press",

number = "2",

}

TY - JOUR

T1 - Murine CD1d-restricted T cell recognition of cellular lipids

AU - Gumperz, Jenny E.

AU - Christopher, Roy

AU - Makowska, Anna

AU - Lum, Deirdre

AU - Sugita, Masahiko

AU - Podrebarac, Theresa

AU - Koezuka, Yasuhiko

AU - Porcelli, Steven A.

AU - Cardell, Susanna

AU - Brenner, Michael B.

AU - Behar, Samuel M.

N1 - Funding Information: J. E. G. is a Burroughs Wellcome Fellow of the Life Sciences Research Foundation. M. S. was suported by the Leukemia Society of America, T. P. by the Medical Research Council of Canada, and S. P. by the American Cancer Society and Arthritis Foundation. This work was supported by National Institutes of Health grants AR01978 to S. M. B. and AI28973 to M. B. B.

PY - 2000/2

Y1 - 2000/2

N2 - NKT cells are associated with immunological control of autoimmune disease and cancer and can recognize cell surface mCD1d without addition of exogenous antigens. Cellular antigens presented by mCD1d have not been identified, although NKT cells can recognize a synthetic glycolipid, α- GalCer. Here we show that after addition of a lipid extract from a tumor cell line, plate-bound mCD1d molecules stimulated an NKT cell hybridoma. This hybridoma also responded strongly to three purified phospholipids, but failed to recognize α-GalCer. Seven of sixteen other mCD1d restricted hybridomas also showed a response to certain purified phospholipids. These findings suggest NKT cells can recognize cellular antigens distinct from α-GalCer and identify phospholipids as potential self-antigens presented by mCD1d.

AB - NKT cells are associated with immunological control of autoimmune disease and cancer and can recognize cell surface mCD1d without addition of exogenous antigens. Cellular antigens presented by mCD1d have not been identified, although NKT cells can recognize a synthetic glycolipid, α- GalCer. Here we show that after addition of a lipid extract from a tumor cell line, plate-bound mCD1d molecules stimulated an NKT cell hybridoma. This hybridoma also responded strongly to three purified phospholipids, but failed to recognize α-GalCer. Seven of sixteen other mCD1d restricted hybridomas also showed a response to certain purified phospholipids. These findings suggest NKT cells can recognize cellular antigens distinct from α-GalCer and identify phospholipids as potential self-antigens presented by mCD1d.

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JO - Immunity

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Murine CD1d-restricted T cell recognition of cellular lipids

Abstract

ASJC Scopus subject areas

UN SDGs

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