@inbook{832465629c6f4b66b578507c434191fe,
title = "Multiplex imaging of Rho family GTPase activities in living cells",
abstract = "Here, we provide procedures for imaging the Rho GTPase biosensors in both single and multiplex acquisition modes. The multiplex approach enables the direct visualization of two biosensor readouts from a single living cell. Here, we take as an example a combination of the RhoA biosensor based on a CFP/YFP FRET modality and the Cdc42 biosensor based on organic dyes that change fluorescence as a function of the local solvent polarity. We list the required optical components as well as cellular manipulation techniques necessary to successfully image these two ratiometric biosensors in a single living cell.",
keywords = "Biosensors, Dynamics, FRET, Live-cell imaging, Multiplex, Rho GTPase",
author = "D{\'e}sir{\'e}e Spiering and Louis Hodgson",
year = "2012",
doi = "10.1007/978-1-61779-442-1_15",
language = "English (US)",
isbn = "9781617794414",
series = "Methods in Molecular Biology",
pages = "215--234",
editor = "Francisco Rivero",
booktitle = "Rho GTPases",
}