Multiple intracellular routes in the cross-presentation of a soluble protein by murine dendritic cells

Deborah Palliser, Eduardo Guillien, Mindy Ju, Herman N. Eisen

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Soluble heat shock fusion proteins (Hsfp) stimulate mice to produce CD8+ CTL, indicating that these proteins are cross-presented by dendritic cells (DC) to naive CD8 T cells. We report that cross-presentation of these proteins depends upon their binding to DC receptors, likely belonging to the scavenger receptor superfamily. Hsfp entered DC by receptor-mediated endocytosis that was either inhibitable by cytochalasin D or not inhabitable, depending upon aggregation state and time. Most endocytosed Hsfp was transported to lysosomes, but not the small cross-presented fraction that exited early from the endocytic pathway and required access to proteasomes and TAP. Naive CD8 T cell (2C and OT-I) responses to DC incubated with Hsfp at 1 μM were matched by incubating DC with cognate octapeptides at 1-10 pM, indicating that display of very few class I MHC-peptide complexes per DC can be sufficient for cross-presentation. With an Hsfp (heat shock proiein-OVA) having peptide sequences for both CD4+ (OT-II) and CD8+ (OT-I) cells, the CD4 cells responded far more vigorously than the CD8 cells and many more class II MHC-peptide than class I MHC-peptide complexes were displayed.

Original languageEnglish (US)
Pages (from-to)1879-1887
Number of pages9
JournalJournal of Immunology
Volume174
Issue number4
StatePublished - Feb 15 2005
Externally publishedYes

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Cross-Priming
Dendritic Cells
Heat-Shock Proteins
Peptides
Proteins
Endocytosis
T-Lymphocytes
Cytochalasin D
Scavenger Receptors
Proteasome Endopeptidase Complex
Lysosomes
Shock
Hot Temperature

ASJC Scopus subject areas

  • Immunology

Cite this

Palliser, D., Guillien, E., Ju, M., & Eisen, H. N. (2005). Multiple intracellular routes in the cross-presentation of a soluble protein by murine dendritic cells. Journal of Immunology, 174(4), 1879-1887.

Multiple intracellular routes in the cross-presentation of a soluble protein by murine dendritic cells. / Palliser, Deborah; Guillien, Eduardo; Ju, Mindy; Eisen, Herman N.

In: Journal of Immunology, Vol. 174, No. 4, 15.02.2005, p. 1879-1887.

Research output: Contribution to journalArticle

Palliser, D, Guillien, E, Ju, M & Eisen, HN 2005, 'Multiple intracellular routes in the cross-presentation of a soluble protein by murine dendritic cells', Journal of Immunology, vol. 174, no. 4, pp. 1879-1887.
Palliser, Deborah ; Guillien, Eduardo ; Ju, Mindy ; Eisen, Herman N. / Multiple intracellular routes in the cross-presentation of a soluble protein by murine dendritic cells. In: Journal of Immunology. 2005 ; Vol. 174, No. 4. pp. 1879-1887.
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