TY - JOUR
T1 - Multiple expression of Ly-6C and accumulation of a Ly-6C pre-mRNA in activated macrophages involved in rejection of an allografted tumor
AU - Takikawa, Osamu
AU - Oku, Tohru
AU - Ito, Naoto
AU - Ushio, Yumiko
AU - Yamamoto, Naoki
AU - Yoneda, Yukio
AU - Tsuji, Junko
AU - Sanchez-Bueno, Antonio
AU - Verkhusha, Vladyslav
AU - Yoshida, Ryotaro
N1 - Funding Information:
1This work was supported in part by research grants from Grants-in Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan; the Vehicle Racing Commemorative Foundation; and Special Coordination Funds of the Science and Technology Agency of the Japanese Government. The nucleotide sequence data of pK30 cDNA reported in this paper will appear in the DDBJ/EMBL/GenBank nucleotide database with the following accession number: D86232.
PY - 1996/9/4
Y1 - 1996/9/4
N2 - We established a monoclonal antibody against a surface antigen of activated macrophages (Mos) involved in the rejection of an allografted mouse tumor, Meth A. Bacteria-elicited Mos also expressed the antigen but resident or inflammatory Mos, or other leukocytes, did not. The antigen was identified as Ly-6C by isolation of cDNA clones encoding it. Two-dimensional immunoblotting revealed that Ly-6C of the Mos exists in multiple forms with a similar size but with different isoelectric points. A Ly-6C pre-mRNA also accumulated abundantly in the Mos. Thus, the multiple expression of Ly-6C and the accumulation of the pre-mRNA are the features of allograft- and bacteria-elicited Mos.
AB - We established a monoclonal antibody against a surface antigen of activated macrophages (Mos) involved in the rejection of an allografted mouse tumor, Meth A. Bacteria-elicited Mos also expressed the antigen but resident or inflammatory Mos, or other leukocytes, did not. The antigen was identified as Ly-6C by isolation of cDNA clones encoding it. Two-dimensional immunoblotting revealed that Ly-6C of the Mos exists in multiple forms with a similar size but with different isoelectric points. A Ly-6C pre-mRNA also accumulated abundantly in the Mos. Thus, the multiple expression of Ly-6C and the accumulation of the pre-mRNA are the features of allograft- and bacteria-elicited Mos.
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U2 - 10.1006/bbrc.1996.1341
DO - 10.1006/bbrc.1996.1341
M3 - Article
C2 - 8806622
AN - SCOPUS:0030568822
SN - 0006-291X
VL - 226
SP - 247
EP - 253
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -