Multiple expression of Ly-6C and accumulation of a Ly-6C pre-mRNA in activated macrophages involved in rejection of an allografted tumor

Osamu Takikawa, Tohru Oku, Naoto Ito, Yumiko Ushio, Naoki Yamamoto, Yukio Yoneda, Junko Tsuji, Antonio Sanchez-Bueno, Vladyslav Verkhusha, Ryotaro Yoshida

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

We established a monoclonal antibody against a surface antigen of activated macrophages (Mos) involved in the rejection of an allografted mouse tumor, Meth A. Bacteria-elicited Mos also expressed the antigen but resident or inflammatory Mos, or other leukocytes, did not. The antigen was identified as Ly-6C by isolation of cDNA clones encoding it. Two-dimensional immunoblotting revealed that Ly-6C of the Mos exists in multiple forms with a similar size but with different isoelectric points. A Ly-6C pre-mRNA also accumulated abundantly in the Mos. Thus, the multiple expression of Ly-6C and the accumulation of the pre-mRNA are the features of allograft- and bacteria-elicited Mos.

Original languageEnglish (US)
Pages (from-to)247-253
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume226
Issue number1
DOIs
StatePublished - Sep 4 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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