Multiple expression of Ly-6C and accumulation of a Ly-6C pre-mRNA in activated macrophages involved in rejection of an allografted tumor

Osamu Takikawa, Tohru Oku, Naoto Ito, Yumiko Ushio, Naoki Yamamoto, Yukio Yoneda, Junko Tsuji, Antonio Sanchez-Bueno, Vladislav Verkhusha, Ryotaro Yoshida

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We established a monoclonal antibody against a surface antigen of activated macrophages (Mos) involved in the rejection of an allografted mouse tumor, Meth A. Bacteria-elicited Mos also expressed the antigen but resident or inflammatory Mos, or other leukocytes, did not. The antigen was identified as Ly-6C by isolation of cDNA clones encoding it. Two-dimensional immunoblotting revealed that Ly-6C of the Mos exists in multiple forms with a similar size but with different isoelectric points. A Ly-6C pre-mRNA also accumulated abundantly in the Mos. Thus, the multiple expression of Ly-6C and the accumulation of the pre-mRNA are the features of allograft- and bacteria-elicited Mos.

Original languageEnglish (US)
Pages (from-to)247-253
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume226
Issue number1
DOIs
StatePublished - Sep 4 1996
Externally publishedYes

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Macrophages
RNA Precursors
Tumors
Neoplasms
Bacteria
Antigens
Isoelectric Point
Surface Antigens
Immunoblotting
Allografts
Leukocytes
Complementary DNA
Clone Cells
Monoclonal Antibodies

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Multiple expression of Ly-6C and accumulation of a Ly-6C pre-mRNA in activated macrophages involved in rejection of an allografted tumor. / Takikawa, Osamu; Oku, Tohru; Ito, Naoto; Ushio, Yumiko; Yamamoto, Naoki; Yoneda, Yukio; Tsuji, Junko; Sanchez-Bueno, Antonio; Verkhusha, Vladislav; Yoshida, Ryotaro.

In: Biochemical and Biophysical Research Communications, Vol. 226, No. 1, 04.09.1996, p. 247-253.

Research output: Contribution to journalArticle

Takikawa, Osamu ; Oku, Tohru ; Ito, Naoto ; Ushio, Yumiko ; Yamamoto, Naoki ; Yoneda, Yukio ; Tsuji, Junko ; Sanchez-Bueno, Antonio ; Verkhusha, Vladislav ; Yoshida, Ryotaro. / Multiple expression of Ly-6C and accumulation of a Ly-6C pre-mRNA in activated macrophages involved in rejection of an allografted tumor. In: Biochemical and Biophysical Research Communications. 1996 ; Vol. 226, No. 1. pp. 247-253.
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AU - Ushio, Yumiko

AU - Yamamoto, Naoki

AU - Yoneda, Yukio

AU - Tsuji, Junko

AU - Sanchez-Bueno, Antonio

AU - Verkhusha, Vladislav

AU - Yoshida, Ryotaro

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AB - We established a monoclonal antibody against a surface antigen of activated macrophages (Mos) involved in the rejection of an allografted mouse tumor, Meth A. Bacteria-elicited Mos also expressed the antigen but resident or inflammatory Mos, or other leukocytes, did not. The antigen was identified as Ly-6C by isolation of cDNA clones encoding it. Two-dimensional immunoblotting revealed that Ly-6C of the Mos exists in multiple forms with a similar size but with different isoelectric points. A Ly-6C pre-mRNA also accumulated abundantly in the Mos. Thus, the multiple expression of Ly-6C and the accumulation of the pre-mRNA are the features of allograft- and bacteria-elicited Mos.

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