We established a monoclonal antibody against a surface antigen of activated macrophages (Mos) involved in the rejection of an allografted mouse tumor, Meth A. Bacteria-elicited Mos also expressed the antigen but resident or inflammatory Mos, or other leukocytes, did not. The antigen was identified as Ly-6C by isolation of cDNA clones encoding it. Two-dimensional immunoblotting revealed that Ly-6C of the Mos exists in multiple forms with a similar size but with different isoelectric points. A Ly-6C pre-mRNA also accumulated abundantly in the Mos. Thus, the multiple expression of Ly-6C and the accumulation of the pre-mRNA are the features of allograft- and bacteria-elicited Mos.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Sep 4 1996|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology