Multiparametric analysis of amino acids and organic acids in rat brain tissues using GC/MS

Jingchao Lin, Mingming Su, Xiaoyan Wang, Yunping Qiu, Houkai Li, Jin Hao, Hongzhou Yang, Mingmei Zhou, Chao Yan, Wei Jia

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Using design of experiment (DOE) theory coupled with multivariate statistical analysis, we have developed a simple and reliable GC/ MS-based analytical assay for simultaneous analysis of amino acids and organic acids in rat brain tissue samples. The process of water extraction (pH 10.0) was extensively evaluated using brain tissue samples and a set of 21 reference standards. Acceptable calibration curves were obtained over a wide concentration range, 0.2-35.0 μg/mL for standards and 15.0-2.4 mL/g (tissue) for brain tissue samples. The precision was mostly better than 10% for both the mixed standards and the brain tissue samples. The brain tissue samples exhibited good stability within 48 h with RSD generally less than 15%. Furthermore, the developed analytical method was successfully applied in distinguishing the subtle variation among different parts of the brain tissues, such as cerebral cortex, hippocampus, and thalamus.

Original languageEnglish (US)
Pages (from-to)2831-2838
Number of pages8
JournalJournal of Separation Science
Volume31
Issue number15
DOIs
StatePublished - Aug 2008
Externally publishedYes

Fingerprint

Organic acids
Amino acids
Rats
Brain
Tissue
Amino Acids
Acids
Thalamus
Cerebral Cortex
Design of experiments
Calibration
Assays
Hippocampus
Statistical methods
Multivariate Analysis
Water

Keywords

  • Amino acid
  • Brain tissue
  • Extraction
  • GC/MS
  • Organic acid

ASJC Scopus subject areas

  • Molecular Medicine
  • Clinical Biochemistry
  • Chemical Engineering(all)
  • Filtration and Separation

Cite this

Multiparametric analysis of amino acids and organic acids in rat brain tissues using GC/MS. / Lin, Jingchao; Su, Mingming; Wang, Xiaoyan; Qiu, Yunping; Li, Houkai; Hao, Jin; Yang, Hongzhou; Zhou, Mingmei; Yan, Chao; Jia, Wei.

In: Journal of Separation Science, Vol. 31, No. 15, 08.2008, p. 2831-2838.

Research output: Contribution to journalArticle

Lin, J, Su, M, Wang, X, Qiu, Y, Li, H, Hao, J, Yang, H, Zhou, M, Yan, C & Jia, W 2008, 'Multiparametric analysis of amino acids and organic acids in rat brain tissues using GC/MS', Journal of Separation Science, vol. 31, no. 15, pp. 2831-2838. https://doi.org/10.1002/jssc.200800232
Lin, Jingchao ; Su, Mingming ; Wang, Xiaoyan ; Qiu, Yunping ; Li, Houkai ; Hao, Jin ; Yang, Hongzhou ; Zhou, Mingmei ; Yan, Chao ; Jia, Wei. / Multiparametric analysis of amino acids and organic acids in rat brain tissues using GC/MS. In: Journal of Separation Science. 2008 ; Vol. 31, No. 15. pp. 2831-2838.
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