Mucosal immunization with attenuated Shigella flexneri harboring an influenza hemagglutinin DNA vaccine protects mice against a lethal influenza challenge

William H. Vecino, Natalie M. Quanquin, Luis Martinez-Sobrido, Ana Fernandez-Sesma, Adolfo García-Sastre, William R. Jacobs, Glenn J. Fennelly

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Mucosal surfaces are important for the induction of immunity against influenza virus. In a murine intranasal immunization model, we demonstrated that the attenuated Shigella flexneri Δasd strain 15D, carrying a DNA construct encoding the influenza virus hemagglutinin (HA), induces protective immunity against a lethal respiratory challenge with influenza A/WSN/33. Influenza virus-specific IFN-γ T cells were detected among splenocytes, and anti-HA IgG was detected in serum post-immunization, albeit at low levels. Following influenza virus challenge, an accelerated anti-HA IgA antibody response was detected in bronchoalveolar lavage (BAL) washings from mice vaccinated with attenuated shigella containing the HA construct. These results suggest that S. flexneri Δasd strain 15D is a promising vector for mucosal DNA vaccine immunization against influenza virus and other mucosal pathogens.

Original languageEnglish (US)
Pages (from-to)192-199
Number of pages8
JournalVirology
Volume325
Issue number2
DOIs
Publication statusPublished - Aug 1 2004

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Keywords

  • Attenuated shigella
  • Bacterial vector
  • DNA vaccines
  • Hemagglutinin
  • IFN-γ
  • Influenza virus
  • Mucosal immunity
  • Pandemic
  • T cell

ASJC Scopus subject areas

  • Virology

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