Monovalent cations mediate formation of native tertiary structure of the Tetrahymena thermophila ribozyme

Keiji Takamoto, Qin He, Stephanie Morris, Mark R. Chance, Michael D. Brenowitz

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The formation of individual tertiary contacts of the Tetrahymena L-21 Sca I ribozyme has been monitored by hydroxyl radical footprinting and its global conformation by analytical ultracentrifugation as a function of monovalent ion concentration in the absence of divalent ions. Advanced methods of data analysis, which allow the hydroxyl radical reactivity of every nucleotide to be quantified, permit monitoring of each and every structural element of the RNA. Monovalent ion-mediated global compaction of the ribozyme is accompanied by the formation of native tertiary contacts; most native tertiary contacts are evident except several that are located near where divalent ions are observed in crystallographic structures. Non-native tertiary contacts are also observed at low but not high concentrations of monovalent ions. In light of recent studies that have shown that the presence of monovalent ions greatly accelerates the Mg2+-dependent folding of the Tetrahymena ribozyme, the present studies suggest that Na+ concentration changes not only the starting position of the RNA on its folding funnel but also pushes it deep into the well by forming native tertiary contacts and, thus, favoring fast and correct folding pathways.

Original languageEnglish (US)
Pages (from-to)928-933
Number of pages6
JournalNature Structural Biology
Volume9
Issue number12
DOIs
StatePublished - Dec 1 2002

Fingerprint

Tetrahymena thermophila
Monovalent Cations
Catalytic RNA
Ions
Tetrahymena
Hydroxyl Radical
RNA
Ultracentrifugation
Conformations
Compaction
Nucleotides
Monitoring

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Genetics

Cite this

Monovalent cations mediate formation of native tertiary structure of the Tetrahymena thermophila ribozyme. / Takamoto, Keiji; He, Qin; Morris, Stephanie; Chance, Mark R.; Brenowitz, Michael D.

In: Nature Structural Biology, Vol. 9, No. 12, 01.12.2002, p. 928-933.

Research output: Contribution to journalArticle

Takamoto, Keiji ; He, Qin ; Morris, Stephanie ; Chance, Mark R. ; Brenowitz, Michael D. / Monovalent cations mediate formation of native tertiary structure of the Tetrahymena thermophila ribozyme. In: Nature Structural Biology. 2002 ; Vol. 9, No. 12. pp. 928-933.
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