Monophosphoryl lipid a protects against gram-positive sepsis and tumor necrosis factor

Mark E. Astiz, Angel Galera, Dhanonjoy C. Saha, Charles Carpati, Eric C. Rackow

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Monophosphoryl lipid A (MPL) is a less toxic derivative of lipid A that enhances survival from endotoxemla. This study examined whether MPL induced resistance to Gram-positive sepsis and cytokines. Mice were administered MPL or saline (phosphate-buffered saline) and challenged 24 h later with live Staphylococcus aureus (SA), staphylococcus enterotoxin B (SEB), toxic shock syndrome toxin (TSST-1), and tumor necrosis factor (TNF). Survival was determined at 72 h. A separate set of animals was phlebotomized for determination of cytokines. MPL Increased survival from S. aureus bacteremia from 20 to 87% (p < .05). lnterieukin-6 (IL-6) and interleukin-1 (IL-1) and TNF were also significantly decreased. SEB and TSST survival were enhanced from 10 to 90% (p < .05). In SEB-treated animals, TNF and IL-6 levels were significantly decreased. Survival from TNF infusion was increased from 20 to 100% with MPL, however, no significant differences in cytokines were observed. These data suggest that MPL induces resistance to Gram-positive sepsis and cytokine-mediated activity.

Original languageEnglish (US)
Pages (from-to)271-274
Number of pages4
JournalShock
Volume2
Issue number4
DOIs
StatePublished - Oct 1994
Externally publishedYes

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine

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