Molecular interaction of CD1b with lipoglycan antigens

William A. Ernst, Juli Maher, Sungae Cho, Kayvan R. Niazi, Delphi Chatterjee, D. Branch Moody, Gurdyal S. Besra, Yutaka Watanabe, Peter E. Jensen, Steven A. Porcelli, Mitchell Kronenberg, Robert L. Modlin

Research output: Contribution to journalArticle

165 Citations (Scopus)

Abstract

The ability of human CD1b molecules to present non-peptide antigens is suggested by the T cell recognition of microbial lipids and lipoglycans in the presence of CD1b-expressing antigen-presenting cells. We demonstrate the high-affinity interaction of CD1b molecules with the acyl side chains of known T cell antigens, lipoarabinomannan, phosphatidylinositol mannoside, and glucose monomycolate. Furthermore, CD1b-antigen binding was optimal at acidic pH, consistent with the known requirement for endosomal acidification in CD1b-restricted antigen presentation. The mechanism for CD1b-ligand interaction involves the partial unfolding of the α helices of CD1b at acidic pH, revealing a hydrophobic binding site that could accommodate lipid. These data provide direct evidence that the CD1b molecule has evolved unique biochemical properties that enable the binding of lipid-containing antigens from intracellular pathogens.

Original languageEnglish (US)
Pages (from-to)331-340
Number of pages10
JournalImmunity
Volume8
Issue number3
DOIs
StatePublished - 1998
Externally publishedYes

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Lipopolysaccharides
Lipids
Antigens
T-Lymphocytes
Viral Tumor Antigens
Antigen Presentation
Antigen-Presenting Cells
Binding Sites
Ligands
CD1b antigen
lipoarabinomannan
phosphatidylinositol mannoside
glucose mycolate

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

Ernst, W. A., Maher, J., Cho, S., Niazi, K. R., Chatterjee, D., Moody, D. B., ... Modlin, R. L. (1998). Molecular interaction of CD1b with lipoglycan antigens. Immunity, 8(3), 331-340. https://doi.org/10.1016/S1074-7613(00)80538-5

Molecular interaction of CD1b with lipoglycan antigens. / Ernst, William A.; Maher, Juli; Cho, Sungae; Niazi, Kayvan R.; Chatterjee, Delphi; Moody, D. Branch; Besra, Gurdyal S.; Watanabe, Yutaka; Jensen, Peter E.; Porcelli, Steven A.; Kronenberg, Mitchell; Modlin, Robert L.

In: Immunity, Vol. 8, No. 3, 1998, p. 331-340.

Research output: Contribution to journalArticle

Ernst, WA, Maher, J, Cho, S, Niazi, KR, Chatterjee, D, Moody, DB, Besra, GS, Watanabe, Y, Jensen, PE, Porcelli, SA, Kronenberg, M & Modlin, RL 1998, 'Molecular interaction of CD1b with lipoglycan antigens', Immunity, vol. 8, no. 3, pp. 331-340. https://doi.org/10.1016/S1074-7613(00)80538-5
Ernst WA, Maher J, Cho S, Niazi KR, Chatterjee D, Moody DB et al. Molecular interaction of CD1b with lipoglycan antigens. Immunity. 1998;8(3):331-340. https://doi.org/10.1016/S1074-7613(00)80538-5
Ernst, William A. ; Maher, Juli ; Cho, Sungae ; Niazi, Kayvan R. ; Chatterjee, Delphi ; Moody, D. Branch ; Besra, Gurdyal S. ; Watanabe, Yutaka ; Jensen, Peter E. ; Porcelli, Steven A. ; Kronenberg, Mitchell ; Modlin, Robert L. / Molecular interaction of CD1b with lipoglycan antigens. In: Immunity. 1998 ; Vol. 8, No. 3. pp. 331-340.
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