Molecular flow resonance Raman effect from retinal and rhodopsin

Robert Callender, A. Doukas, R. Crouch, K. Nakanishi

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

We have performed resonance enhanced Raman measurements of retinal isomers in solution (all-trans, 11-cis, 9-cis, and 13-cis) and cetyltrimethylammonium bromide (CTAB) detergent extracts of bovine rhodopsin near physiological temperatures (17°C). In order to measure these photolabile systems, we have developed a general technique which allows Raman measurements of any photosensitive material. This technique involves imposing a molecular velocity transverse to the Raman exciting laser beam sufficient to ensure that any given molecule moves through the beam so that it has little probability of absorbing a photon. We have also measured the resonance Raman spectra of crystals of the same retinal isomers. The data show that each isomer has a distinct and characteristic Raman spectra and that the spectrum of 11-cis-retinal is quite similar but not identical with that of rhodopsin and similarly for 9-cis-retinal compared with isorhodopsin. In agreement with previous work, the Raman data demonstrate that retinal and opsin are joined by a protonated Schiff base. Due to the fact that the Raman spectra of 11-cis-retinal (solution) and rhodopsin show bands near 998 and 1018 cm-1, a spectral region previously assigned to C-Me stretching motions, it is suggested that 11-cis-retinal in solution is composed of a mixture of 12-s-trans and 12-s-cis, and that the conformation of rhodopsin is (perhaps distorted) 12-s-trans.

Original languageEnglish (US)
Pages (from-to)1621-1629
Number of pages9
JournalBiochemistry
Volume15
Issue number8
StatePublished - 1976
Externally publishedYes

Fingerprint

Rhodopsin
Retinaldehyde
Raman scattering
Isomers
Opsins
Schiff Bases
Photons
Detergents
Stretching
Laser beams
Conformations
Lasers
Crystals
Molecules
Temperature

ASJC Scopus subject areas

  • Biochemistry

Cite this

Callender, R., Doukas, A., Crouch, R., & Nakanishi, K. (1976). Molecular flow resonance Raman effect from retinal and rhodopsin. Biochemistry, 15(8), 1621-1629.

Molecular flow resonance Raman effect from retinal and rhodopsin. / Callender, Robert; Doukas, A.; Crouch, R.; Nakanishi, K.

In: Biochemistry, Vol. 15, No. 8, 1976, p. 1621-1629.

Research output: Contribution to journalArticle

Callender, R, Doukas, A, Crouch, R & Nakanishi, K 1976, 'Molecular flow resonance Raman effect from retinal and rhodopsin', Biochemistry, vol. 15, no. 8, pp. 1621-1629.
Callender R, Doukas A, Crouch R, Nakanishi K. Molecular flow resonance Raman effect from retinal and rhodopsin. Biochemistry. 1976;15(8):1621-1629.
Callender, Robert ; Doukas, A. ; Crouch, R. ; Nakanishi, K. / Molecular flow resonance Raman effect from retinal and rhodopsin. In: Biochemistry. 1976 ; Vol. 15, No. 8. pp. 1621-1629.
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