Molecular cloning of the interleukin-1β converting enzyme

Douglas Pat Cerretti, Carl J. Kozlosky, Bruce Mosley, Nicole Nelson, Kirk Van Ness, Teresa A. Greenstreet, Carl J. March, Shirley R. Kronheim, Teresa Druck, Linda A. Cannizzaro, Kay Huebner, Roy A. Black

Research output: Contribution to journalArticle

916 Citations (Scopus)

Abstract

Interleukin-1β (IL-1β) mediates a wide range of immune and inflammatory responses. The active cytokine is generated by proteolytic cleavage of an inactive precursor. A complementary DNA encoding a protease that carries out this cleavage has been cloned. Recombinant expression in COS-7 cells enabled the cells to process precursor IL-1β to the mature form. Sequence analysis indicated that the enzyme itself may undergo proteolytic processing. The gene encoding the protease was mapped to chromosomal band 11q23, a site frequently involved in rearrangement in human cancers.

Original languageEnglish (US)
Pages (from-to)97-100
Number of pages4
JournalScience
Volume256
Issue number5053
StatePublished - Apr 3 1992
Externally publishedYes

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Caspase 1
Molecular Cloning
Peptide Hydrolases
COS Cells
Interleukin-1
Sequence Analysis
Complementary DNA
Cytokines
Enzymes
Genes
Neoplasms
interleukin 1 precursor

ASJC Scopus subject areas

  • General

Cite this

Cerretti, D. P., Kozlosky, C. J., Mosley, B., Nelson, N., Van Ness, K., Greenstreet, T. A., ... Black, R. A. (1992). Molecular cloning of the interleukin-1β converting enzyme. Science, 256(5053), 97-100.

Molecular cloning of the interleukin-1β converting enzyme. / Cerretti, Douglas Pat; Kozlosky, Carl J.; Mosley, Bruce; Nelson, Nicole; Van Ness, Kirk; Greenstreet, Teresa A.; March, Carl J.; Kronheim, Shirley R.; Druck, Teresa; Cannizzaro, Linda A.; Huebner, Kay; Black, Roy A.

In: Science, Vol. 256, No. 5053, 03.04.1992, p. 97-100.

Research output: Contribution to journalArticle

Cerretti, DP, Kozlosky, CJ, Mosley, B, Nelson, N, Van Ness, K, Greenstreet, TA, March, CJ, Kronheim, SR, Druck, T, Cannizzaro, LA, Huebner, K & Black, RA 1992, 'Molecular cloning of the interleukin-1β converting enzyme', Science, vol. 256, no. 5053, pp. 97-100.
Cerretti DP, Kozlosky CJ, Mosley B, Nelson N, Van Ness K, Greenstreet TA et al. Molecular cloning of the interleukin-1β converting enzyme. Science. 1992 Apr 3;256(5053):97-100.
Cerretti, Douglas Pat ; Kozlosky, Carl J. ; Mosley, Bruce ; Nelson, Nicole ; Van Ness, Kirk ; Greenstreet, Teresa A. ; March, Carl J. ; Kronheim, Shirley R. ; Druck, Teresa ; Cannizzaro, Linda A. ; Huebner, Kay ; Black, Roy A. / Molecular cloning of the interleukin-1β converting enzyme. In: Science. 1992 ; Vol. 256, No. 5053. pp. 97-100.
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