Molecular cloning of the interleukin-1β converting enzyme

Douglas Pat Cerretti; Carl J. Kozlosky; Bruce Mosley; Nicole Nelson; Kirk Van Ness; Teresa A. Greenstreet; Carl J. March; Shirley R. Kronheim; Teresa Druck; Linda A. Cannizzaro; Kay Huebner; Roy A. Black

doi:10.1126/science.1373520

Molecular cloning of the interleukin-1β converting enzyme

Douglas Pat Cerretti, Carl J. Kozlosky, Bruce Mosley, Nicole Nelson, Kirk Van Ness, Teresa A. Greenstreet, Carl J. March, Shirley R. Kronheim, Teresa Druck, Linda A. Cannizzaro, Kay Huebner, Roy A. Black

Pathology

Research output: Contribution to journal › Article › peer-review

698 Scopus citations

Abstract

Interleukin-1β (IL-1β) mediates a wide range of immune and inflammatory responses. The active cytokine is generated by proteolytic cleavage of an inactive precursor. A complementary DNA encoding a protease that carries out this cleavage has been cloned. Recombinant expression in COS-7 cells enabled the cells to process precursor IL-1β to the mature form. Sequence analysis indicated that the enzyme itself may undergo proteolytic processing. The gene encoding the protease was mapped to chromosomal band 11q23, a site frequently involved in rearrangement in human cancers.

Original language	English (US)
Pages (from-to)	97-100
Number of pages	4
Journal	Science
Volume	256
Issue number	5053
DOIs	https://doi.org/10.1126/science.1373520
State	Published - 1992

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10.1126/science.1373520

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title = "Molecular cloning of the interleukin-1β converting enzyme",

abstract = "Interleukin-1β (IL-1β) mediates a wide range of immune and inflammatory responses. The active cytokine is generated by proteolytic cleavage of an inactive precursor. A complementary DNA encoding a protease that carries out this cleavage has been cloned. Recombinant expression in COS-7 cells enabled the cells to process precursor IL-1β to the mature form. Sequence analysis indicated that the enzyme itself may undergo proteolytic processing. The gene encoding the protease was mapped to chromosomal band 11q23, a site frequently involved in rearrangement in human cancers.",

author = "Cerretti, {Douglas Pat} and Kozlosky, {Carl J.} and Bruce Mosley and Nicole Nelson and {Van Ness}, Kirk and Greenstreet, {Teresa A.} and March, {Carl J.} and Kronheim, {Shirley R.} and Teresa Druck and Cannizzaro, {Linda A.} and Kay Huebner and Black, {Roy A.}",

year = "1992",

doi = "10.1126/science.1373520",

language = "English (US)",

volume = "256",

pages = "97--100",

journal = "Science",

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T1 - Molecular cloning of the interleukin-1β converting enzyme

AU - Cerretti, Douglas Pat

AU - Kozlosky, Carl J.

AU - Mosley, Bruce

AU - Nelson, Nicole

AU - Van Ness, Kirk

AU - Greenstreet, Teresa A.

AU - March, Carl J.

AU - Kronheim, Shirley R.

AU - Druck, Teresa

AU - Cannizzaro, Linda A.

AU - Huebner, Kay

AU - Black, Roy A.

PY - 1992

Y1 - 1992

N2 - Interleukin-1β (IL-1β) mediates a wide range of immune and inflammatory responses. The active cytokine is generated by proteolytic cleavage of an inactive precursor. A complementary DNA encoding a protease that carries out this cleavage has been cloned. Recombinant expression in COS-7 cells enabled the cells to process precursor IL-1β to the mature form. Sequence analysis indicated that the enzyme itself may undergo proteolytic processing. The gene encoding the protease was mapped to chromosomal band 11q23, a site frequently involved in rearrangement in human cancers.

AB - Interleukin-1β (IL-1β) mediates a wide range of immune and inflammatory responses. The active cytokine is generated by proteolytic cleavage of an inactive precursor. A complementary DNA encoding a protease that carries out this cleavage has been cloned. Recombinant expression in COS-7 cells enabled the cells to process precursor IL-1β to the mature form. Sequence analysis indicated that the enzyme itself may undergo proteolytic processing. The gene encoding the protease was mapped to chromosomal band 11q23, a site frequently involved in rearrangement in human cancers.

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VL - 256

SP - 97

EP - 100

JO - Science

JF - Science

IS - 5053

ER -

Molecular cloning of the interleukin-1β converting enzyme

Abstract

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UN SDGs

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