Molecular cloning of cDNAs for the human granulocyte colony-stimulating factor receptor from HL-60 and mapping of the gene to chromosome region 1p32-34

David J. Tweardy, Kristin Anderson, Linda A. Cannizzaro, Richard A. Steinman, Carlo M. Croce, Kay Huebner

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Early studies examining the effects of purified or recombinant granulocyte colony-stimulating factor (G-CSF) on human leukemia cell lines demonstrated that some cell lines, such as HL-60, could be induced to differentiate in response to G-CSF. In two recent studies reporting the cloning of the human G-CSF receptor (hGCSFR), four classes of receptor cDNA were identified and, surprisingly, the message for this receptor was reportedly expressed by HL-60 at either very low levels or not at all. Using a mouse G-CSF receptor probe, we cloned and sequenced a cDNA for hGCSFR from an HL-60 cDNA library in plasmid and used it to identify 31 additional clones from an HL-60 cDNA library in phage. Polymerase chain reaction analysis of the 31 phage clones established that 29 were derived from class I hGCSFR mRNA, one was derived from class III mRNA, and one was derived from class IV mRNA. In addition, the hGCSFR gene was chromosomally localized by Southern blot analysis of its segregation pattern in a panel of rodent-human hybrid DNAs using the radiolabeled cDNA probe. The hGCSFR locus was present in hybrids retaining the distal short arm of human chromosome 1 and absent in hybrids that did not retain this region. Chromosomal in situ hybridization refined the localization of the hGCSFR gene to region 1p32-p34. The combination of hybrid DNA analysis and in situ hybridization places the hGCSFR gene telomeric to the CSF1, JUN, and TCL-5 loci.

Original languageEnglish (US)
Pages (from-to)1148-1154
Number of pages7
JournalBlood
Volume79
Issue number5
StatePublished - Mar 1 1992
Externally publishedYes

Fingerprint

Granulocyte Colony-Stimulating Factor Receptors
Chromosome Mapping
Cloning
Molecular Cloning
Chromosomes
Complementary DNA
Genes
Bacteriophages
Granulocyte Colony-Stimulating Factor
Gene Library
Messenger RNA
In Situ Hybridization
Clone Cells
Cells
Cell Line
Chromosomes, Human, Pair 1
Polymerase chain reaction
DNA
Human Chromosomes
Southern Blotting

ASJC Scopus subject areas

  • Hematology

Cite this

Tweardy, D. J., Anderson, K., Cannizzaro, L. A., Steinman, R. A., Croce, C. M., & Huebner, K. (1992). Molecular cloning of cDNAs for the human granulocyte colony-stimulating factor receptor from HL-60 and mapping of the gene to chromosome region 1p32-34. Blood, 79(5), 1148-1154.

Molecular cloning of cDNAs for the human granulocyte colony-stimulating factor receptor from HL-60 and mapping of the gene to chromosome region 1p32-34. / Tweardy, David J.; Anderson, Kristin; Cannizzaro, Linda A.; Steinman, Richard A.; Croce, Carlo M.; Huebner, Kay.

In: Blood, Vol. 79, No. 5, 01.03.1992, p. 1148-1154.

Research output: Contribution to journalArticle

Tweardy, DJ, Anderson, K, Cannizzaro, LA, Steinman, RA, Croce, CM & Huebner, K 1992, 'Molecular cloning of cDNAs for the human granulocyte colony-stimulating factor receptor from HL-60 and mapping of the gene to chromosome region 1p32-34', Blood, vol. 79, no. 5, pp. 1148-1154.
Tweardy DJ, Anderson K, Cannizzaro LA, Steinman RA, Croce CM, Huebner K. Molecular cloning of cDNAs for the human granulocyte colony-stimulating factor receptor from HL-60 and mapping of the gene to chromosome region 1p32-34. Blood. 1992 Mar 1;79(5):1148-1154.
Tweardy, David J. ; Anderson, Kristin ; Cannizzaro, Linda A. ; Steinman, Richard A. ; Croce, Carlo M. ; Huebner, Kay. / Molecular cloning of cDNAs for the human granulocyte colony-stimulating factor receptor from HL-60 and mapping of the gene to chromosome region 1p32-34. In: Blood. 1992 ; Vol. 79, No. 5. pp. 1148-1154.
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