Molecular characterization of immunoreactivities of peptides derived from chromogranin a (GE-25) and from secretogranin II (secretoneurin) in human and bovine cerebrospinal fluid

R. Kirchmair, A. Benzer, J. Troger, C. Miller, J. Marksteiner, A. Saria, R. W. Gasser, R. Hogue-Angeletti, R. Fischer-Colbrie, H. Winkler

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Abstract

Chromogranin A and secretogranin II are members of the so-called chromogranins, the acidic proteins stored in neuroendocrine large dense-core vesicles. We characterized chromogranin A and secretogranin II immunoreactivities in cerebrospinal fluid by radioimmunoassays using synthetic peptides derived from these components (GE-25 for chromogranin A and secretoneurin for secretogranin II). In lumbar cerebrospinal fluid, high levels (more than 1000 fmol/ml) of these two components were found, whereas in ventricular cerebrospinal fluid the secretoneurin levels were relatively low. The cerebrospinal fluid/serum ratio for secretoneurin was close to 170. High-performance liquid chromatography revealed that in both cerebrospinal fluid and extracts from human brain secretoneurin was the predominant immunoreactive component. In cerebrospinal fluid chromogranin A immunoreactivity was present as intermediate-sized peptides with little intact chromogranin A and free GE-25 peptide. In human brain samples smaller peptides including GE-25 were more predominant. Analogous findings for secretoneurin and chromogranin A were obtained for bovine brain samples. We can conclude that chromogranins are present in cerebrospinal fluid in concentrations much higher than those of classical neuropeptides also stored in large dense-core vesicles. Therefore, their degree of proteolytic processing can be analysed with small samples of cerebrospinal fluid. A possible disturbance of proteolytic processing in large dense-core vesicles in various pathological conditions can now be discovered.

Original languageEnglish (US)
Pages (from-to)1179-1187
Number of pages9
JournalNeuroscience
Volume63
Issue number4
DOIs
StatePublished - 1994

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Secretogranin II
Chromogranins
Chromogranin A
Cerebrospinal Fluid
Peptides
Secretory Vesicles
Brain
secretoneurin
Neuropeptides
Radioimmunoassay
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Neuroscience(all)

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Molecular characterization of immunoreactivities of peptides derived from chromogranin a (GE-25) and from secretogranin II (secretoneurin) in human and bovine cerebrospinal fluid. / Kirchmair, R.; Benzer, A.; Troger, J.; Miller, C.; Marksteiner, J.; Saria, A.; Gasser, R. W.; Hogue-Angeletti, R.; Fischer-Colbrie, R.; Winkler, H.

In: Neuroscience, Vol. 63, No. 4, 1994, p. 1179-1187.

Research output: Contribution to journalArticle

Kirchmair, R, Benzer, A, Troger, J, Miller, C, Marksteiner, J, Saria, A, Gasser, RW, Hogue-Angeletti, R, Fischer-Colbrie, R & Winkler, H 1994, 'Molecular characterization of immunoreactivities of peptides derived from chromogranin a (GE-25) and from secretogranin II (secretoneurin) in human and bovine cerebrospinal fluid', Neuroscience, vol. 63, no. 4, pp. 1179-1187. https://doi.org/10.1016/0306-4522(94)90582-7
Kirchmair, R. ; Benzer, A. ; Troger, J. ; Miller, C. ; Marksteiner, J. ; Saria, A. ; Gasser, R. W. ; Hogue-Angeletti, R. ; Fischer-Colbrie, R. ; Winkler, H. / Molecular characterization of immunoreactivities of peptides derived from chromogranin a (GE-25) and from secretogranin II (secretoneurin) in human and bovine cerebrospinal fluid. In: Neuroscience. 1994 ; Vol. 63, No. 4. pp. 1179-1187.
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AU - Troger, J.

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AU - Marksteiner, J.

AU - Saria, A.

AU - Gasser, R. W.

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