Molecular biology of PCP and NMDA receptors.

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The studies described demonstrate that rat brain mRNA directs the synthesis of at least four types of functional EAA receptors in the Xenopus oocyte system, whereas in this system NCB-20 cell mRNA directs the synthesis of only the NMDA type of EAA receptor. The NMDA channel expressed in the oocyte, using either rat brain mRNA or NCB-20 cell mRNA, exhibits the pharmacologic properties of the neuronal receptor, including the functional association with the PCP receptor located within the NMDA-gated channel. The demonstration that mRNA isolated from NCB-20 cells lacking functional NMDA-activated channels, but bearing PCP binding sites, can encode functional NMDA-activated channels in the oocyte indicates some defect or regulating step in posttranslational processing or insertion of the receptors into the plasma membrane in the cell of origin. This is the only cell line known to (1) have PCP receptors that appear to be associated with NMDA receptors and (2) provide a homogeneous, self-replicating population of cells that can be manipulated genetically and by changing the extracellular environment. Consequently, the NCB-20 cell line will be useful for the study of the NMDA receptor and its expression.

Original languageEnglish (US)
Pages (from-to)159-183
Number of pages25
JournalNIDA research monograph
Volume133
StatePublished - 1993

Fingerprint

Phencyclidine Receptors
N-Methyl-D-Aspartate Receptors
Molecular Biology
N-Methylaspartate
Messenger RNA
Oocytes
Cell Line
Brain
Xenopus
Binding Sites
Cell Membrane
Population

ASJC Scopus subject areas

  • Medicine (miscellaneous)

Cite this

Molecular biology of PCP and NMDA receptors. / Kushner, L.; Bennett, Michael V. L.; Zukin, R. Suzanne.

In: NIDA research monograph, Vol. 133, 1993, p. 159-183.

Research output: Contribution to journalArticle

@article{615be002e8b14dc18aa62909312040c6,
title = "Molecular biology of PCP and NMDA receptors.",
abstract = "The studies described demonstrate that rat brain mRNA directs the synthesis of at least four types of functional EAA receptors in the Xenopus oocyte system, whereas in this system NCB-20 cell mRNA directs the synthesis of only the NMDA type of EAA receptor. The NMDA channel expressed in the oocyte, using either rat brain mRNA or NCB-20 cell mRNA, exhibits the pharmacologic properties of the neuronal receptor, including the functional association with the PCP receptor located within the NMDA-gated channel. The demonstration that mRNA isolated from NCB-20 cells lacking functional NMDA-activated channels, but bearing PCP binding sites, can encode functional NMDA-activated channels in the oocyte indicates some defect or regulating step in posttranslational processing or insertion of the receptors into the plasma membrane in the cell of origin. This is the only cell line known to (1) have PCP receptors that appear to be associated with NMDA receptors and (2) provide a homogeneous, self-replicating population of cells that can be manipulated genetically and by changing the extracellular environment. Consequently, the NCB-20 cell line will be useful for the study of the NMDA receptor and its expression.",
author = "L. Kushner and Bennett, {Michael V. L.} and Zukin, {R. Suzanne}",
year = "1993",
language = "English (US)",
volume = "133",
pages = "159--183",
journal = "NIDA Research Monograph Series",
issn = "1046-9516",
publisher = "National Institute on Drug Abuse",

}

TY - JOUR

T1 - Molecular biology of PCP and NMDA receptors.

AU - Kushner, L.

AU - Bennett, Michael V. L.

AU - Zukin, R. Suzanne

PY - 1993

Y1 - 1993

N2 - The studies described demonstrate that rat brain mRNA directs the synthesis of at least four types of functional EAA receptors in the Xenopus oocyte system, whereas in this system NCB-20 cell mRNA directs the synthesis of only the NMDA type of EAA receptor. The NMDA channel expressed in the oocyte, using either rat brain mRNA or NCB-20 cell mRNA, exhibits the pharmacologic properties of the neuronal receptor, including the functional association with the PCP receptor located within the NMDA-gated channel. The demonstration that mRNA isolated from NCB-20 cells lacking functional NMDA-activated channels, but bearing PCP binding sites, can encode functional NMDA-activated channels in the oocyte indicates some defect or regulating step in posttranslational processing or insertion of the receptors into the plasma membrane in the cell of origin. This is the only cell line known to (1) have PCP receptors that appear to be associated with NMDA receptors and (2) provide a homogeneous, self-replicating population of cells that can be manipulated genetically and by changing the extracellular environment. Consequently, the NCB-20 cell line will be useful for the study of the NMDA receptor and its expression.

AB - The studies described demonstrate that rat brain mRNA directs the synthesis of at least four types of functional EAA receptors in the Xenopus oocyte system, whereas in this system NCB-20 cell mRNA directs the synthesis of only the NMDA type of EAA receptor. The NMDA channel expressed in the oocyte, using either rat brain mRNA or NCB-20 cell mRNA, exhibits the pharmacologic properties of the neuronal receptor, including the functional association with the PCP receptor located within the NMDA-gated channel. The demonstration that mRNA isolated from NCB-20 cells lacking functional NMDA-activated channels, but bearing PCP binding sites, can encode functional NMDA-activated channels in the oocyte indicates some defect or regulating step in posttranslational processing or insertion of the receptors into the plasma membrane in the cell of origin. This is the only cell line known to (1) have PCP receptors that appear to be associated with NMDA receptors and (2) provide a homogeneous, self-replicating population of cells that can be manipulated genetically and by changing the extracellular environment. Consequently, the NCB-20 cell line will be useful for the study of the NMDA receptor and its expression.

UR - http://www.scopus.com/inward/record.url?scp=0027340602&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027340602&partnerID=8YFLogxK

M3 - Article

VL - 133

SP - 159

EP - 183

JO - NIDA Research Monograph Series

JF - NIDA Research Monograph Series

SN - 1046-9516

ER -