Molecular and Functional Asymmetry at a Vertebrate Electrical Synapse

JohnE Rash, Sebastian Curti, KimberlyG Vanderpool, Naomi Kamasawa, Srikant Nannapaneni, Nicolas Palacios-Prado, CarmenE Flores, Thomas Yasumura, John O'Brien, BruceD Lynn, Feliksas F. Bukauskas, JamesI Nagy, Alberto E. Pereda

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Electrical synapses are abundant in the vertebrate brain, but their functional and molecular complexities are still poorly understood. We report here that electrical synapses between auditory afferents and goldfish Mauthner cells are constructed by apposition of hemichannels formed by two homologs of mammalian connexin 36 (Cx36) and that, while Cx35 is restricted to presynaptic hemiplaques, Cx34.7 is restricted to postsynaptic hemiplaques, forming heterotypic junctions. This molecular asymmetry is associated with rectification of electrical transmission that may act to promote cooperativity between auditory afferents. Our data suggest that, in similarity to pre- and postsynaptic sites at chemical synapses, one side in electrical synapses should not necessarily be considered the mirror image of the other. While asymmetry based on the presence of two Cx36 homologs is restricted to teleost fish, it might also be based on differences in posttranslational modifications of individual connexins or in the complement of gap junction-associated proteins

Original languageEnglish (US)
Pages (from-to)957-969
Number of pages13
JournalNeuron
Volume79
Issue number5
DOIs
StatePublished - Sep 4 2013

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Electrical Synapses
Vertebrates
Connexins
Goldfish
Post Translational Protein Processing
Synapses
Fishes
Brain
connexin 36

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Rash, J., Curti, S., Vanderpool, K., Kamasawa, N., Nannapaneni, S., Palacios-Prado, N., ... Pereda, A. E. (2013). Molecular and Functional Asymmetry at a Vertebrate Electrical Synapse. Neuron, 79(5), 957-969. https://doi.org/10.1016/j.neuron.2013.06.037

Molecular and Functional Asymmetry at a Vertebrate Electrical Synapse. / Rash, JohnE; Curti, Sebastian; Vanderpool, KimberlyG; Kamasawa, Naomi; Nannapaneni, Srikant; Palacios-Prado, Nicolas; Flores, CarmenE; Yasumura, Thomas; O'Brien, John; Lynn, BruceD; Bukauskas, Feliksas F.; Nagy, JamesI; Pereda, Alberto E.

In: Neuron, Vol. 79, No. 5, 04.09.2013, p. 957-969.

Research output: Contribution to journalArticle

Rash, J, Curti, S, Vanderpool, K, Kamasawa, N, Nannapaneni, S, Palacios-Prado, N, Flores, C, Yasumura, T, O'Brien, J, Lynn, B, Bukauskas, FF, Nagy, J & Pereda, AE 2013, 'Molecular and Functional Asymmetry at a Vertebrate Electrical Synapse', Neuron, vol. 79, no. 5, pp. 957-969. https://doi.org/10.1016/j.neuron.2013.06.037
Rash J, Curti S, Vanderpool K, Kamasawa N, Nannapaneni S, Palacios-Prado N et al. Molecular and Functional Asymmetry at a Vertebrate Electrical Synapse. Neuron. 2013 Sep 4;79(5):957-969. https://doi.org/10.1016/j.neuron.2013.06.037
Rash, JohnE ; Curti, Sebastian ; Vanderpool, KimberlyG ; Kamasawa, Naomi ; Nannapaneni, Srikant ; Palacios-Prado, Nicolas ; Flores, CarmenE ; Yasumura, Thomas ; O'Brien, John ; Lynn, BruceD ; Bukauskas, Feliksas F. ; Nagy, JamesI ; Pereda, Alberto E. / Molecular and Functional Asymmetry at a Vertebrate Electrical Synapse. In: Neuron. 2013 ; Vol. 79, No. 5. pp. 957-969.
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