Molecular analysis of scabrous mutant alleles from Drosophila melanogaster indicates a secreted protein with two functional domains

X. Hu, E. C. Lee, N. E. Baker

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Mutations at the scabrous locus (sca) affect cell-cell signaling during neural development. Twenty-one mutant alleles of scabrous have been analyzed. Many synthesize no sca protein. In others, a defective protein is arrested intracellularly. Two mutants in which protein is not arrested must affect sca protein function outside the cell. Both affect the fibrinogen related domain (FRcD), a 200-amino acid segment conserved in fibrinogen, tenascins, and other proteins. In fibrinogen, this region is involved in protein interactions and is altered in human mutations affecting blood clotting. In sca(UM2), an invariant Asp residue is replaced by Asn. In sca(MSKF), an insertion of the hobo transposable element truncates the sca protein at the start of the FRcD. The sca(MSKF) allele has dominant negative properties, indicating that the truncated amino-terminal portion interferes with the function of some other gene product. These mutations show that the conserved FRcD is essential for wild-type sca function, but suggest that the amino- terminal domain also interacts with other proteins. Genetic interactions identify the neurogenic genes Notch and Delta as potential interacting proteins, but other neural mutations were without effect. Models for the role of a two-domain protein in neural development are discussed.

Original languageEnglish (US)
Pages (from-to)607-617
Number of pages11
JournalGenetics
Volume141
Issue number2
Publication statusPublished - Jan 1 1995

    Fingerprint

ASJC Scopus subject areas

  • Genetics

Cite this