Model Studies of DNA Photorepair

Enthalpy of Cleavage of a Pyrimidine Dimer Measured by Photothermal Beam Deflection Calorimetry

Michael P. Scannell, Syun-Ru Yeh, Daniel E. Falvey

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The enzyme DNA photolyase mediates the repair of pyrimidine dimers. This repair step, a net retro [2+2] reaction, proceeds through either the cation or anion radical of the pyrimidine dimer. In order to understand how electron transfer makes the repair process possible, its energetics have been examined by photothermal beam deflection calorimetry, fluorescence quenching and quantum yield studies. The enthalpy for the cleavage reaction of cis-syn 1,3-dimethylthymine dimer itself was found to be -19 kcal/mol. In addition, from the redox potentials, the enthalpies for the cleavage reactions of the dimer cation radical and the anion radical were determined to be -19 kcal/mol and -28 kcal/mol, respectively.

Original languageEnglish (US)
Pages (from-to)764-768
Number of pages5
JournalPhotochemistry and Photobiology
Volume64
Issue number5
StatePublished - Nov 1996
Externally publishedYes

Fingerprint

Pyrimidine Dimers
Calorimetry
pyrimidines
Anions
Cations
deflection
cleavage
Enthalpy
Repair
heat measurement
deoxyribonucleic acid
Deoxyribodipyrimidine Photo-Lyase
enthalpy
dimers
Dimers
DNA
Oxidation-Reduction
Fluorescence
Quantum yield
Electrons

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Model Studies of DNA Photorepair : Enthalpy of Cleavage of a Pyrimidine Dimer Measured by Photothermal Beam Deflection Calorimetry. / Scannell, Michael P.; Yeh, Syun-Ru; Falvey, Daniel E.

In: Photochemistry and Photobiology, Vol. 64, No. 5, 11.1996, p. 764-768.

Research output: Contribution to journalArticle

@article{0caef1630e8c4605bae3d5f8ae0c8570,
title = "Model Studies of DNA Photorepair: Enthalpy of Cleavage of a Pyrimidine Dimer Measured by Photothermal Beam Deflection Calorimetry",
abstract = "The enzyme DNA photolyase mediates the repair of pyrimidine dimers. This repair step, a net retro [2+2] reaction, proceeds through either the cation or anion radical of the pyrimidine dimer. In order to understand how electron transfer makes the repair process possible, its energetics have been examined by photothermal beam deflection calorimetry, fluorescence quenching and quantum yield studies. The enthalpy for the cleavage reaction of cis-syn 1,3-dimethylthymine dimer itself was found to be -19 kcal/mol. In addition, from the redox potentials, the enthalpies for the cleavage reactions of the dimer cation radical and the anion radical were determined to be -19 kcal/mol and -28 kcal/mol, respectively.",
author = "Scannell, {Michael P.} and Syun-Ru Yeh and Falvey, {Daniel E.}",
year = "1996",
month = "11",
language = "English (US)",
volume = "64",
pages = "764--768",
journal = "Photochemistry and Photobiology",
issn = "0031-8655",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Model Studies of DNA Photorepair

T2 - Enthalpy of Cleavage of a Pyrimidine Dimer Measured by Photothermal Beam Deflection Calorimetry

AU - Scannell, Michael P.

AU - Yeh, Syun-Ru

AU - Falvey, Daniel E.

PY - 1996/11

Y1 - 1996/11

N2 - The enzyme DNA photolyase mediates the repair of pyrimidine dimers. This repair step, a net retro [2+2] reaction, proceeds through either the cation or anion radical of the pyrimidine dimer. In order to understand how electron transfer makes the repair process possible, its energetics have been examined by photothermal beam deflection calorimetry, fluorescence quenching and quantum yield studies. The enthalpy for the cleavage reaction of cis-syn 1,3-dimethylthymine dimer itself was found to be -19 kcal/mol. In addition, from the redox potentials, the enthalpies for the cleavage reactions of the dimer cation radical and the anion radical were determined to be -19 kcal/mol and -28 kcal/mol, respectively.

AB - The enzyme DNA photolyase mediates the repair of pyrimidine dimers. This repair step, a net retro [2+2] reaction, proceeds through either the cation or anion radical of the pyrimidine dimer. In order to understand how electron transfer makes the repair process possible, its energetics have been examined by photothermal beam deflection calorimetry, fluorescence quenching and quantum yield studies. The enthalpy for the cleavage reaction of cis-syn 1,3-dimethylthymine dimer itself was found to be -19 kcal/mol. In addition, from the redox potentials, the enthalpies for the cleavage reactions of the dimer cation radical and the anion radical were determined to be -19 kcal/mol and -28 kcal/mol, respectively.

UR - http://www.scopus.com/inward/record.url?scp=0030294543&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030294543&partnerID=8YFLogxK

M3 - Article

VL - 64

SP - 764

EP - 768

JO - Photochemistry and Photobiology

JF - Photochemistry and Photobiology

SN - 0031-8655

IS - 5

ER -