Model Studies of DNA Photorepair: Enthalpy of Cleavage of a Pyrimidine Dimer Measured by Photothermal Beam Deflection Calorimetry

Michael P. Scannell, Syun Ru Yeh, Daniel E. Falvey

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The enzyme DNA photolyase mediates the repair of pyrimidine dimers. This repair step, a net retro [2+2] reaction, proceeds through either the cation or anion radical of the pyrimidine dimer. In order to understand how electron transfer makes the repair process possible, its energetics have been examined by photothermal beam deflection calorimetry, fluorescence quenching and quantum yield studies. The enthalpy for the cleavage reaction of cis-syn 1,3-dimethylthymine dimer itself was found to be -19 kcal/mol. In addition, from the redox potentials, the enthalpies for the cleavage reactions of the dimer cation radical and the anion radical were determined to be -19 kcal/mol and -28 kcal/mol, respectively.

Original languageEnglish (US)
Pages (from-to)764-768
Number of pages5
JournalPhotochemistry and Photobiology
Volume64
Issue number5
DOIs
StatePublished - Nov 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

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