@article{8525c317e208465abe6ec6ba3d867027,
title = "Mitochondrial-type hsp70 genes of the amitochondriate protists, Giardia intestinalis, Entamoeba histolytica and two microsporidians",
abstract = "Genes encoding putative mitochondrial-type heat shock protein 70 (mit-hsp70) were isolated and sequenced from amitochondriate protists, Giardia intestinalis, Entamoeba histolytica, and two microsporidians, Encephalitozoon hellem and Glugea plecoglossi. The deduced mit-hsp70 sequences were analyzed by sequence alignments and phylogenetic reconstructions. The mit-hsp70 sequence of these four amitochondriate protists were divergent from other mit-hsp70 sequences of mitochondriate eukaryotes. However, all of these sequences were clearly located within a eukaryotic mitochondrial clade in the tree including various type hsp70 sequences, supporting the emerging notion that none of these amitochondriate lineages are primitively amitochodrial, but lost their mitochondria secondarily in their evolutionary past.",
keywords = "Amitochondriate protists, Entamoeba, Giardia, Hsp70, Microsporidia, Phylogeny",
author = "Nobuko Arisue and S{\'a}nchez, {Lidya B.} and Weiss, {Louis M.} and Mikl{\'o}s M{\"u}ller and Tetsuo Hashimoto",
note = "Funding Information: We thank Dr H.S. Zhang for extracting genomic DNA of Encephalitozoon hellem and for preparing its genomic DNA library, Dr G. Wu, Dr M. Hasegawa and Dr T. Yano for helpful discussions, and A. Deguchi and S. Kikuchi for technical assistance. This work was carried out under the ISM Co-operative Research Program (00ISMC·RP-2042, and 01ISM·CRP-2038), and was supported by grants from the Japanese Society for the Promotion of Science (10044219 and 13640709) to T.H. Work carried out at the Rockefeller University in New York was supported by the USPHS National Institutes of Health Grant (AI11942) to M.M. Work carried out at the Albert Einstein College of Medicine in New York was supported by the USPHS National Institutes of Health Grant (AI31788) to L.M.W. Visits of T.H. to the New York laboratories and of L.B.S. to the Hayama laboratory were supported by the US–Japan Co-operative Research Project by National Science Foundation and Japanese Society for the Promotion of Science (INT-9726707). Preliminary sequence data for E. histolytica is deposited regularly into the GSS division of GenBank. The sequencing effort is part of the International Entamoeba Genome Sequencing Project and is supported by an award from the National Institute of Allergy and Infectious Diseases, National Institutes of Health.",
year = "2002",
doi = "10.1016/S1383-5769(01)00093-9",
language = "English (US)",
volume = "51",
pages = "9--16",
journal = "Parasitology International",
issn = "1383-5769",
publisher = "Elsevier Ireland Ltd",
number = "1",
}
